Topic
Bacteriophage phi 6
About: Bacteriophage phi 6 is a research topic. Over the lifetime, 85 publications have been published within this topic receiving 232968 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Abstract: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.
232,912 citations
TL;DR: The purification and properties of a lipid-containing bacteriophage, phi6, are described, which contains a lipid envelope which is probably essential for infection.
Abstract: The purification and properties of a lipid-containing bacteriophage, phi6, are described. The phage contains a lipid envelope which is probably essential for infection. Infectivity of phi6 was lost in the presence of organic solvents, sodium deoxycholate, and phospholipase A. The fatty acid composition of the phage lipid was similar to that of the Pseudomonas phaseolicola host cells. The phage was composed of about 25% lipid, 13% RNA, and 62% protein. The buoyant density of phi6 was 1.27 g/ml in cesium chloride. The morphology of phi6 was unusual; it had a polyhedral head of about 60 nm surrounded by a membranous, compressible envelope which appeared to assume an elongated configuration upon attachment to pili. The adsorption rate constant was 3.3 x 10(-10) ml/min in a semi-synthetic medium and 3.8 x 10(-10) ml/min in a nutrient broth-yeast extract medium. The latent period was shorter in the former medium (80-115 min compared with 120-160 min), and the average burst size was larger (250-400 compared with 125-150). The eclipse period coincided with the latent period.
364 citations
TL;DR: The nucleic acid component of bacteriophage φ6 is characterized as a double stranded RNA molecule with a buoyant density of 1.605 g/cm 3 and nucleotide composition of C, 27.3%; A, 21.8%; G, 28.9%; and U, 22.0%.
155 citations
TL;DR: The enveloped dsRNA bacteriophage phi 6 uses the pilus of Pseudomonas syringae as its receptor and it was shown that at phage/host cell ratios of 0.1-1 practically every virion produces an infectious center.
125 citations
TL;DR: The organization of the P1 molecules in the unexpanded and expanded polymerase complex suggests that the expansion is accomplished by rigid body movements of theP1 monomers, which leads to exposure of new potential RNA binding surfaces to control the sequential packaging of the genome segments.
121 citations