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Bessel beam

About: Bessel beam is a research topic. Over the lifetime, 1946 publications have been published within this topic receiving 42264 citations.


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Journal ArticleDOI
TL;DR: An effective microoperating system for single cell manipulation using microtube arrays is constructed, and its use in the capture, transfer, and release of embryonic fibroblast mouse cells as well as human breast cancer cells is demonstrated.
Abstract: In this paper, we present a focused femtosecond laser Bessel beam scanning technique for the rapid fabrication of large-area 3D complex microtube arrays. The femtosecond laser beam is converted into several Bessel beams by two-dimensional phase modulation using a spatial light modulator. By scanning the focused Bessel beam along a designed route, microtubes with variable size and flexible geometry are rapidly fabricated by two-photon polymerization. The fabrication time is reduced by two orders of magnitude in comparison with conventional point-to-point scanning. Moreover, we construct an effective microoperating system for single cell manipulation using microtube arrays, and demonstrate its use in the capture, transfer, and release of embryonic fibroblast mouse cells as well as human breast cancer cells. The new fabrication strategy provides a novel method for the rapid fabrication of functional devices using a flexibly tailored laser beam.

31 citations

Journal ArticleDOI
TL;DR: Scintillation aspects of truncated Bessel beams propagated through atmospheric turbulence are investigated using a numerical wave optics random phase screen simulation method and it is found that in almost all circumstances studied, the zeroth-order Bessel beam will deliver the lowest scintillation.
Abstract: Scintillation aspects of truncated Bessel beams propagated through atmospheric turbulence are investigated using a numerical wave optics random phase screen simulation method. On-axis, aperture averaged scintillation and scintillation relative to a classical Gaussian beam of equal source power and scintillation per unit received power are evaluated. It is found that in almost all circumstances studied, the zeroth-order Bessel beam will deliver the lowest scintillation. Low aperture averaged scintillation levels are also observed for the fourth-order Bessel beam truncated by a narrower source window. When assessed relative to the scintillation of a Gaussian beam of equal source power, Bessel beams generally have less scintillation, particularly at small receiver aperture sizes and small beam orders. Upon including in this relative performance measure the criteria of per unit received power, this advantageous position of Bessel beams mostly disappears, but zeroth- and first-order Bessel beams continue to offer some advantage for relatively smaller aperture sizes, larger source powers, larger source plane dimensions, and intermediate propagation lengths.

31 citations

Journal ArticleDOI
TL;DR: This work reports how Bessel beams reduce streaking artifacts and produce high-fidelity quantitative data demonstrating a fivefold increase in sensitivity to calcium transients and a 20- fold increase in accuracy in the detection of activity correlations in functional imaging.
Abstract: Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a choice method to observe brain function with high frame rates at cellular resolution. Inherently to LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest and, during functional imaging, modulate fluorescence variations related to neuronal activity. Here, we report how Bessel beams reduce streaking artifacts and produce high-fidelity quantitative data demonstrating a fivefold increase in sensitivity to calcium transients and a 20 fold increase in accuracy in the detection of activity correlations in functional imaging. Furthermore, using principal component analysis, we show that measurements obtained with Bessel beams are clean enough to reveal in one-shot experiments correlations that can not be averaged over trials after stimuli as is the case when studying spontaneous activity. Our results not only demonstrate the contamination of data by systematic and random errors through conventional Gaussian illumination and but,furthermore, quantify the increase in fidelity of such data when using Bessel beams.

31 citations

Journal ArticleDOI
TL;DR: An efficient numerical method based on surface integral equations to characterize the scattering of a zero-order Bessel beam by arbitrarily shaped homogeneous dielectric particles is introduced.
Abstract: In this paper, we introduce an efficient numerical method based on surface integral equations to characterize the scattering of a zero-order Bessel beam by arbitrarily shaped homogeneous dielectric particles. The incident beam is described by vector expressions in terms of the electric and magnetic fields that perfectly satisfy Maxwell’s equations. The scattering problems involving homogeneous dielectric particles with arbitrary shapes are formulated with the electric and magnetic current combined-field integral equation and modeled by using surface triangular patches. Solutions are performed iteratively by using the multilevel fast multipole algorithm. Some numerical results are included to illustrate the validity and capability of the proposed method. These results are also expected to provide useful insights into the scattering of a Bessel beam by complex-shaped particles.

31 citations

Journal ArticleDOI
TL;DR: The use of a light droplet illumination whose side lobes are suppressed by interfering Bessel beams of specific k-vectors is demonstrated, providing a more efficient energy localization without loss in transverse resolution.
Abstract: An ideal illumination for light sheet fluorescence microscopy entails both a localized and a propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions, but their non-diffracting feature comes at the cost of the presence of high-energy side lobes that notably degrade the imaging contrast and induce photobleaching. Here, we demonstrate the use of a light droplet illumination whose side lobes are suppressed by interfering Bessel beams of specific k-vectors. Our droplet illumination readily achieves more than 50% extinction of the light distributed across the Bessel side lobes, providing a more efficient energy localization without loss in transverse resolution. In a standard light sheet fluorescence microscope, we demonstrate a two-fold contrast enhancement imaging micron-scale fluorescent beads. Results pave the way to new opportunities for rapid and deep in vivo observations of large-scale biological systems.

31 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202373
2022149
2021113
2020126
2019134
2018140