Showing papers on "Bioaerosol published in 2021"

The bioaerosols emitted from toilet and wastewater treatment plant: a literature review

Abstract: The aerosols harboring microorganisms and viruses released from the wastewater system into the air have greatly threatened the health and safety of human beings. The wastewater systems, including toilet and wastewater treatment plant (WWTP), are the major locations of epidemic infections due to the extensive sources of aerosols, as well as multifarious germs and microorganisms. Viruses and microorganisms may transport from both toilet and hospital into municipal pipes and subsequently into WWTP, which accounts for the main source of bioaerosols dispersed in the air of the wastewater system. This review aims to elaborate the generation, transmission, and diffusion processes of bioaerosols at toilet and WWTP. Moreover, the main factors affecting bioaerosol transmission and the corresponding prevention strategies for the airborne and inhaled bioaerosols are also discussed. Collectively, this review highlights the importance of managing bioaerosol occurrence in the wastewater system, which has aroused increasing concern from the public.

The removal of airborne SARS-CoV-2 and other microbial bioaerosols by air filtration on COVID-19 surge units

Abstract: Summary Background The COVID-19 pandemic has overwhelmed the respiratory isolation capacity in hospitals; many wards lacking high-frequency air changes have been repurposed for managing patients infected with SARS-CoV-2 requiring either standard or intensive care. Hospital-acquired COVID-19 is a recognised problem amongst both patients and staff, with growing evidence for the relevance of airborne transmission. This study examined the effect of air filtration and ultra-violet (UV) light sterilisation on detectable airborne SARS-CoV-2 and other microbial bioaerosols. Methods We conducted a crossover study of portable air filtration and sterilisation devices in a repurposed ‘surge’ COVID ward and ‘surge’ ICU. National Institute for Occupational Safety and Health (NIOSH) cyclonic aerosol samplers and PCR assays were used to detect the presence of airborne SARS-CoV-2 and other microbial bioaerosol with and without air/UV filtration. Results Airborne SARS-CoV-2 was detected in the ward on all five days before activation of air/UV filtration, but on none of the five days when the air/UV filter was operational; SARS-CoV-2 was again detected on four out of five days when the filter was off. Airborne SARS-CoV-2 was infrequently detected in the ICU. Filtration significantly reduced the burden of other microbial bioaerosols in both the ward (48 pathogens detected before filtration, two after, p=0.05) and the ICU (45 pathogens detected before filtration, five after p=0.05). Conclusions These data demonstrate the feasibility of removing SARS-CoV-2 from the air of repurposed ‘surge’ wards and suggest that air filtration devices may help reduce the risk of hospital-acquired SARS-CoV-2. Funding Wellcome Trust, MRC, NIHR

Short-range bioaerosol deposition and recovery of viable viruses and bacteria on surfaces from a cough and implications for respiratory disease transmission

Abstract: Knowledge of respiratory bacterium/virus distribution on surfaces is critical for studying disease transmission via the contact route. Here, we investigated the bioaerosol deposition and distributi...

Bioaerosols in the landfill environment: an overview of microbial diversity and potential health hazards

Abstract: Landfilling is one of the indispensable parts of solid waste management in various countries. Solid waste disposed of in landfill sites provides nutrients for the proliferation of pathogenic microbes which are aerosolized into the atmosphere due to the local meteorology and various waste disposal activities. Bioaerosols released from landfill sites can create health issues for employees and adjoining public. The present study offers an overview of the microbial diversity reported in the air samples collected from various landfill sites worldwide. This paper also discusses other aspects, including effect of meteorological conditions on the bioaerosol concentrations, sampling techniques, bioaerosol exposure and potential health impacts. Analysis of literature concluded that landfill air is dominated by microbial dust or various pathogenic microbes like Enterobacteriaceae, Staphylococcus aureus, Clostridium perfringens, Acinetobacter calcoaceticus and Aspergillus fumigatus. The bioaerosols present in the landfill environment are of respirable sizes and can penetrate deep into lower respiratory systems and trigger respiratory symptoms and chronic pulmonary diseases. Most studies reported higher bioaerosol concentrations in spring and summer as higher temperature and relative humidity provide a favourable environment for survival and multiplication of microbes. Landfill workers involved in solid waste disposal activities are at the highest risk of exposure to these bioaerosols due to their proximity to solid waste and as they practise minimum personal safety and hygiene measures during working hours. Workers are recommended to use personal protective equipment and practise hygiene to reduce the impact of occupational exposure to bioaerosols.

Aerosol and bioaerosol particle size and dynamics from defective sanitary plumbing systems.

Abstract: Aerosols are readily transported on airstreams through building sanitary plumbing and sewer systems, and those containing microbial pathogens (known as bioaerosols) are recognized as contributors to infection spread within buildings. When a defect occurs in the sanitary plumbing system that affects the system integrity, a cross-transmission route is created that can enable the emission of bioaerosols from the system into the building. These emission occurrences are characterized as short-burst events (typically 99.5%) were 11 μm anywhere in the system. Particle count was influenced by toilet flush volume, but it was not possible to determine if there was any direct influence from airflow rate since both particle and biological data showed no correlation with upward airflow rates and velocities. Typical emissions resulting from a 6 L toilet flush were in the range of 280-400 particles per second at a concentration of typically 9-12 number per cm3 and a total particle count in the region of 3000 to 4000 particles, whereas the peak emissions from a 1.2 L toilet flush were 60-80 particles per second at a concentration of 2.4-3 number per cm3 and a total particle count in the region of 886 to 1045 particles. The reduction in particles is in direct proportion to the reduction in toilet flush volume. The slit-to-agar sampler was able to provide viable time course CFU data and confirmed the origin of the particles to be the tracer microorganism flushed into the system. The time course data also have characteristics consistent with the unsteady nature of a toilet flush.

Quantitative microbial risk assessment of bioaerosols in a wastewater treatment plant by using two aeration modes.

Abstract: Nonnegligible emission of bioaerosols usually occurs during aeration of wastewater in aerator tanks in wastewater treatment plants (WWTPs). Literature had shown that the respiratory and intestinal diseases of workers at WWTPs are related to bioaerosols. Thus, quantitative microbial risk assessment (QMRA) based on Monte Carlo simulation was utilized in this research to assess the health risks of Gram-negative bacteria bioaerosol (GNBB) and Staphylococcus aureus bioaerosol (SAB) among academic visitors and staffs. Results showed that the concentrations of GNBB and SAB in the inverted umbrella aeration mode were consistently higher than those in the microporous aeration mode under all six size distribution ranges of the Anderson six-stage impactor. Thus, GNBB and SAB can be highly threatening to the weasand and first bronchus (or alveoli and third bronchus) for the exposure populations. The health risks (annual probability of infection (Py) and disease burden (DB)) of males were constantly higher than those of females for each certain exposure scenario. The health risks of staffs were higher than those of academic visitors when assessed by Monte Carlo simulation. The wearing of mask is an effective measure to minimize health risks through reducing the bioaerosol concentration intake. Especially, for the academic visitors and staffs exposed to GNBB, all their DB failed to meet the World Health Organization DB benchmark under various credible intervals when they were without a mask on. In a word, the results of health risk assessment in this research can be utilized as an educational tool and policy basis to facilitate the implementation of efficacious prevention measures to protect the public health from bioaerosol health threats in WWTPs.

Bioaerosol Contribution to Atmospheric Particulate Matter in Indoor University Environments

Abstract: Within the framework of the project “Integrated Evaluation of Indoor Particulate Exposure”, we carried out a 4-week field study to determine indoor bioaerosol, and its contribution to particulate matter (PM)10 and organic matter. The study was carried out in university classrooms, where most of the common indoor sources of atmospheric particles are missing. Bioaerosol was determined by a method based on propidium iodide staining, observation by fluorescence microscopy, and image analysis. Indoor bioaerosol concentrations were compared with outdoor values, which were determined simultaneously. The samplings periods were scheduled to divide weekday hours, when the students were inside, from night-time hours and weekends. Very high bioaerosol concentrations were detected inside the classrooms with respect to outdoor values. The mean difference was 49 μg/m3 when the students were inside, 5.4 μg/m3 during the night, and it became negative during the weekends. Indoor-to-outdoor ratios were 6.0, 4.2, and 0.7, respectively. Bioaerosol contributed 26% to organics and 10% to PM10. In indoor samples collected during the day, the microscope images showed numerous skin fragments, which were mostly responsible for the increase in the bioaerosol mass. People’s presence proved to be responsible for a significant increase in bioaerosol concentration in crowded indoor environments.

Assessment of Real-time Bioaerosol Particle Counters using Reference Chamber Experiments

Abstract: . This study presents the first reference calibrations of three commercially available bioaerosol detectors. The Droplet Measurement Technologies WIBS-NEO, Plair Rapid-E, and Swisens Poleno were compared with a primary standard for particle number concentrations at the Federal Institute for Metrology METAS. Polystyrene (PSL) spheres were used to assess absolute particle counts for diameters from 0.5 μm to 10 μm. For the three devices, counting efficiency was found to be strongly dependent on particle size. The results confirm the expected detection range for which the instruments were designed. While the WIBS-NEO achieves its highest efficiency at smaller particles, e.g. 90 % for 0.9 μm diameter, the Plair Rapid-E performs best for larger particles, with an efficiency of 58 % for particles with a diameter of 10 μm. The Swisens Poleno is also designed for larger particles, but operates well from 2 μm. However, the exact counting efficiency of the Poleno could not be evaluated as the cut-off diameter range of the integrated concentrator unit was not completely covered. In further experiments, three different types of fluorescent particles were tested to investigate the fluorescent detection capabilities of the Plair Rapid-E and the Swisens Poleno. Both instruments showed good agreement with the reference data. While the challenge to produce known concentrations of larger particles above 10 μm or even fresh pollen particles remain, the approach presented in this paper provides a potential standardised validation method that can be used to assess counting efficiency and fluorescence measurements of automatic bioaerosol monitoring devices.

Microbial aerosol particles in four seasons of sanitary landfill site: Molecular approaches, traceability and risk assessment.

Abstract: Landfill sites are regarded as prominent sources of bioaerosols for the surrounding atmosphere The present study focused on the emission of airborne bacteria and fungi in four seasons of a sanitary landfill site The main species found in bioaerosols were assayed using high-throughput sequencing The SourceTracker method was utilized to identify the sources of the bioaerosols present at the boundary of the landfill site Furthermore, the health consequences of the exposure to bioaerosols were evaluated based on the average daily dose rates Results showed that the concentrations of airborne bacteria in the operation area (OPA) and the leakage treatment area (LTA) were in the range of (4684 ± 477)-(10883 ± 1395) CFU/m3 and (3179 ± 453)-(9051 ± 738) CFU/m3, respectively The average emission levels of fungal aerosols were 4026 CFU/m3 for OPA and 1295 CFU/m3 for LTA The landfill site received the maximum bioaerosol load during summer and the minimum during winter Approximately 4139%- 8624% of the airborne bacteria had a particle size of 11 to 47 µm, whereas 4827%- 6645% of the airborne fungi had a particle size of more than 47 µm Bacillus sp, Brevibacillus sp, and Paenibacillus sp were abundant in the bacterial population, whereas Penicillium sp and Aspergillus sp dominated the fungal population Bioaerosols released from the working area and treatment of leachate were the two main sources that emerged in the surrounding air of the landfill site boundary The exposure risks during summer and autumn were higher than those in spring and winter

Short-range Bioaerosol Deposition and Inhalation of Cough Droplets and Performance of Personalized Ventilation

Abstract: A short distance between infected persons and exposed persons can probably result in a high risk of respiratory infection. This work experimentally investigated the short-range bioaerosol depositio...

Presence and variability of culturable bioaerosols in three multi-family apartment buildings with different ventilation systems in the Northeastern US.

Abstract: Bioaerosol concentrations in residential buildings located in the Northeastern US have not been widely studied. Here, in 2011-2015, we studied the presence and seasonal variability of culturable fungi and bacteria in three multi-family apartment buildings and correlated the bioaerosol concentrations with building ventilation system types and environmental parameters. A total of 409 indoor and 86 outdoor samples were taken. Eighty-five percent of investigated apartments had indoor-outdoor (I/O) ratios of culturable fungi below 1, suggesting minimal indoor sources of fungi. In contrast, 56% of the apartments had I/O ratios for culturable bacteria above 1, indicating the prominence of indoor sources of bacteria. Culturable fungi I/O ratios in apartments serviced by central heating, ventilation, and air-conditioning (HVAC) system were lower than those in apartments with window AC. The type of ventilation system did not have a significant effect on the presence of indoor culturable bacteria. A significant positive association was determined between indoor dew point (DP) levels and indoor culturable fungi (P < .001) and bacteria (P < .001), regardless of ventilation type. Also, residents in apartments with central HVAC did not experience extreme DP values. We conclude that building ventilation systems, seasonality, and indoor sources are major factors affecting indoor bioaerosol levels in residential buildings.

Enriched Aerosol-to-Hydrosol Transfer for Rapid and Continuous Monitoring of Bioaerosols.

Abstract: Bioaerosols, including infectious diseases such as COVID-19, are a continuous threat to global public safety. Despite their importance, the development of a practical, real-time means of monitoring bioaerosols has remained elusive. Here, we present a novel, simple, and highly efficient means of obtaining enriched bioaerosol samples. Aerosols are collected into a thin and stable liquid film by the unique interaction of a superhydrophilic surface and a continuous two-phase centrifugal flow. We demonstrate that this method can provide a concentration enhancement ratio of ∼2.4 × 106 with a collection efficiency of ∼99.9% and an aerosol-into-liquid transfer rate of ∼95.9% at 500 nm particle size (smaller than a single bacterium). This transfer is effective in both laboratory and external ambient environments. The system has a low limit of detection of <50 CFU/m3air using a straightforward bioluminescence-based technique and shows significant potential for air monitoring in occupational and public-health applications.

Ice Nucleation Activity of Alpine Bioaerosol Emitted in Vicinity of a Birch Forest

Abstract: In alpine environments, many plants, bacteria, and fungi contain ice nuclei (IN) that control freezing events, providing survival benefits. Once airborne, IN could trigger ice nucleation in cloud droplets, influencing the radiation budget and the hydrological cycle. To estimate the atmospheric relevance of alpine IN, investigations near emission sources are inevitable. In this study, we collected 14 aerosol samples over three days in August 2019 at a single site in the Austrian Alps, close to a forest of silver birches, which are known to release IN from their surface. Samples were taken during and after rainfall, as possible trigger of aerosol emission by an impactor and impinger at the ground level. In addition, we collected aerosol samples above the canopy using a rotary wing drone. Samples were analyzed for ice nucleation activity, and bioaerosols were characterized based on morphology and auto-fluorescence using microscopic techniques. We found high concentrations of IN below the canopy, with a freezing behavior similar to birch extracts. Sampled particles showed auto-fluorescent characteristics and the morphology strongly suggested the presence of cellular material. Moreover, some particles appeared to be coated with an organic film. To our knowledge, this is the first investigation of aerosol emission sources in alpine vegetation with a focus on birches.

An opinion review on sampling strategies, enumeration techniques, and critical environmental factors for bioaerosols: An emerging sustainability indicator for society and cities

Abstract: Increasing bio-terror attacks across the world have alarmed the environmentalist and epidemiologist about the impacts of bioaerosols, especially on human health, ecosystems, and live stocks. Further, its fate has also been linked with future sustainability of rural and urban societies. These concerns have led the researchers to have in depth understanding of their sampling approaches, enumeration techniques, and survival factors. In this view, the suitable methods, available for the bioaerosol sampling i.e. filtration, impaction, and impingement are analyzed within the scope of the specific studies. With regard to compositional analysis of bioaerosols, detailed assessment revealed that overall microbial taxonomy cannot be expressed alone by culture-dependent methods, as majority of the microbial species are not culturable. Further, the overall diversity of the bioaerosols may be significantly expressed by means of culture-independent methods, in which various advanced sequencing techniques have resulted into wide range of outcomes in branch of genomics and metagenomics. In particular, the community structure is greatly influenced by the combination of various factors such as the complex interrelations between bacteria and fungi in form of co-occurrence or co-exclusion patterns, air pollution levels, and seasonal variations etc. The important meteorological parameters, responsible for their survival, transport, and contribution to the suspended particulate, include relative humidity, air temperature, wind direction, wind speed, rainfall, solar radiation. The correlation between these factors and bioaerosol concentration was observed to be either positive, negative, or non-significant as it is strongly governed by local weather conditions and seasonal changes of the investigated regions. Moreover, the complex inter-relationships between bioaerosols and environmental pollutants such as sulfur dioxide, carbon monoxide, and ozone also reported to influence the bioaerosol concentration. Overall, this review offers insight into the current state of bioaerosol sampling approaches, devised detection techniques, survival and transport factors, and associated health effects. Authors also encourage that future research should be pursued from a sustainability perspective with a vision of development of reliable sampling methods and detection techniques.

Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones.

Abstract: Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effective ways to limit exposures to them. The objective of this study was to evaluate a variety of impinger and cyclone aerosol or bioaerosol samplers to determine approaches most suitable for detecting and measuring concentrations of virus-containing particles in air. Six impinger/cyclone air samplers, a filter-based sampler, and a cascade impactor were used in separate tests to collect artificially generated aerosols of MS2 bacteriophage and swine and avian influenza viruses. Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. The influenza viruses were titrated in cell cultures to determine quantities of infectious virus. Viral RNA was extracted and used for quantitative real time RT-PCR, to provide total virus concentrations for all three viruses. The amounts of virus recovered and the measured airborne virus concentrations were calculated and compared among the samplers. Not surprisingly, high flow rate samplers generally collected greater quantities of virus than low flow samplers. However, low flow rate samplers generally measured higher, and likely more accurate, airborne concentrations of Infectious virus and viral RNA than high flow samplers. To assess airborne viruses in the field, a two-sampler approach may work well. A suitable high flow sampler may provide low limits of detection to determine if any virus is present in the air. If virus is detected, a suitable lower flow sampler may measure airborne virus concentrations accurately.

Numerical Investigation of Bioaerosol Transport in a Compact Lavatory

Abstract: The lavatory is a fertile area for the transmission of infectious disease through bioaerosols between its users. In this study, we built a generic compact lavatory model with a vacuum toilet, and computational fluid dynamics (CFD) is used to evaluate the effects of ventilation and user behaviors on the airflow patterns, and the resulting fates of bioaerosols. Fecal aerosols are readily released into the lavatory during toilet flush. Their concentration rapidly decays in the first 20 s after flushing by deposition or dilution. It takes about 315 s to 348 s for fine bioaerosols (<10 µm in diameter) to decrease to 5% of the initial concentration, while it takes 50 and 100 µm bioaerosols approximately 11 and <1 s, respectively, to completely deposit. The most contaminated surfaces by aerosol deposition include the toilet seat, the bowl, and the nearby walls. The 10 µm aerosols tend to deposit on horizontal surfaces, while the 50 and 100 µm bioaerosols almost always deposit on the bowl. In the presence of a standing thermal manikin, the rising thermal plume alters the flow field and more bioaerosols are carried out from the toilet; a large fraction of aerosols deposit on the manikin’s legs. The respiratory droplets generated by a seated coughing manikin tend to deposit on the floor, legs, and feet of the manikin. In summary, this study reveals the bioaerosol dilution time and the easily contaminated surfaces in a compact lavatory, which will aid the development of control measures against infectious diseases.

Diurnal Variations of Size-Resolved Bioaerosols During Autumn and Winter Over a Semi-Arid Megacity in Northwest China.

Abstract: Bioaerosols have a major negative effect on air quality and on public health by causing the spread of diseases. This study evaluated the bioaerosol composition and variation in a semi-arid megacity of northwest China from October 2019 to January 2020 using an Andersen six-stage impactor sampler. The size distribution, diurnal variations of the concentrations of airborne bacteria, airborne fungi, and total airborne microbes (TAM) were investigated in autumn and winter. The mean concentrations of airborne bacteria, fungi, and TAM were 523.5 ± 301.1 colony-forming units (CFU)/m3, 1318.9 ± 447.8 CFU/m3, and (7.25 ± 1.90) × 106 cells/m3, respectively, in autumn and 581 ± 305.4 CFU/m3, 1234.4 ± 519.9 CFU/m3, and (5.96 ± 1.65) × 106 cells/m3, respectively, in winter. The mean bioaerosol concentrations were slightly higher on nonhaze days than on haze days, but the difference was not statistically significant. Higher ambient particulate matter levels and atmospheric oxidation capacity inhibited bacteria survival. The diurnal maximum bioaerosol concentration was observed in the morning in autumn, whereas in winter, bioaerosols did not exhibit such a distribution, the impact of human activities on bioaerosols was still uncertain. The size of airborne bacteria exhibited a bimodal distribution, whereas a unimodal pattern was observed for fungi and TAM. Most bacteria, fungi, and TAM were distributed in the respirable ranges from trachea and primary bronchi to alveoli, indicating that bioaerosols have a high risk of being inhaled and causing respiratory diseases in Xi'an.