Topic
Bioaerosol
About: Bioaerosol is a research topic. Over the lifetime, 1347 publications have been published within this topic receiving 34791 citations.
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TL;DR: In this paper, the integration of an aerosol inertial impactor with in situ fluorescence imaging for the real-time detection and monitoring of airborne biological particles was described, where airborne particles were continuously collected on a fluorescent dye-containing agarose gel, and a mini-fluorescence microscope was used to produce in situ fluorescent particle images.
Abstract: We describe the integration of an aerosol inertial impactor with in situ fluorescence imaging for the real-time detection and monitoring of airborne biological particles. Airborne particles were continuously collected on a fluorescent dye-containing agarose gel, and a mini-fluorescence microscope was used to produce in situ fluorescence particle images. Concentrations of size-segregated and dye-stained particles were then measured in real time upon deposition of airborne biological particles on the agarose gel substrate. To evaluate system performance, airborne bacterial particles from Escherichia coli were introduced onto the impactor (termed Bio-impactor) at various air flow rates and collection times. The particle cut-off diameter (d50) was controlled by adjusting sample flow rate so that a sample flow rate of 10 L/min produced a d50 of ∼0.84 μm, which efficiently collected airborne E. coli particles. Our Bio-impactor system allows for the automated fluorescence imaging of collected particles, providing in situ information about fluorescence intensity at regular time intervals. We compared results from our system with those obtained using a colony counting method; our results demonstrated a clear correlation (R2 = 0.9536) between system response and expected particle counts. Our novel impactor system could provide an inexpensive, simple, and portable method of real-time bioaerosol quantification.
16 citations
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01 Jan 2011TL;DR: Cultivation and microscopic counting are traditional analytical methods, but recently several new methods are emerging that are based on chemical, biological, or immunochemical analysis of bioaerosol components.
Abstract: Bioaerosols include viruses, bacteria, fungi, pollen, and their fragments as well as animal allergens. Bioaerosol exposure is common in indoor and outdoor environments and may cause infections, allergies, irritation, and toxic effects. The size of biological particles varies widely, from nanoscale (virions and microbial fragments) to approximately 100 μm (pollen grains). The same physical principles that are applied to nonbiological particles can be applied to bioaerosol sampling in terms of sampling efficiency of a given particle size range. When sampling bioaerosols for exposure assessment purposes, one has to consider what biological property would be the most relevant measure for the health effect in question. Cultivation and microscopic counting are traditional analytical methods, but recently several new methods are emerging that are based on chemical, biological, or immunochemical analysis of bioaerosol components. Data interpretation is based on comparisons of results in target and reference areas or populations. When comparing data with previously published values, only results that are obtained using the same methodology should be directly compared.
16 citations
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01 Jan 2011TL;DR: Three bioaerosol samplers used in this study are not able to alarm Campylobacter outbreaks through an airborne route when low bacterial concentrations are present and developments of new sampling techniques with low detection limits are required for biosecurity assessment.
Abstract: In an airborne transmission experiment, Campylobacter in the air was sampled by three types of bioaerosol samplers (all-glass impinger AGI-30, Andersen six-stage impactor, and OMNI-3000) in four broiler rooms. In each room, five 14-day- old broilers inoculated with Campylobacter jejuni were kept in a central cage located in the middle of the room. Another ten broilers, as susceptible animals, were kept individually in ten cages surrounding the central cage at a distance of approximately 75 cm. Air samples were taken on eight days: the day before inoculation (BI) as a negative control, and 1, 3, 6, 9, 14, 21, and 29 days post-inoculation (PI). Presence of C. jejuni was investigated with the culture method for culturable bacteria and with the PCR test for bacterial DNA. Results showed that Campylobacter infection of susceptible broilers occurred in all four rooms; however, no culturable C. jejuni could be detected in any of the air samples. This might have been the result of the low number of broilers in the room and the unfavorable conditions for Campylobacter survival, leading to Campylobacter concentrations below the detection limits of the bioaerosol samplers. The PCR test showed that DNA of C. jejuni was detected in the air samples on the first day PI, but no bacterial DNA was detected on the following days. It is concluded that the three samplers used in this study are not able to alarm Campylobacter outbreaks through an airborne route when low bacterial concentrations are present. Developments of new sampling techniques with low detection limits are required for biosecurity assessment.
16 citations
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TL;DR: This field study investigated biological characteristics of aerosols emitted from a commercial egg production farm (layer operation) and found that there were significantly lower total bacterial concentrations in the samples collected from ambient stations as compared with the samples Collected in the layer house.
Abstract: This field study investigated biological characteristics of aerosols emitted from a commercial egg production farm (layer operation). Bioaerosol samples were taken on this farm at five sampling locations covering emission source (inside a layer barn) and four ambient surrounding stations at four wind directions. All-glass impingers (AGI) were used for the field sampling. AGI fluid samples were plated in duplicate on Trypticase Soy Agar for growth of bacteria and Sabouraud Dextrose Agar for growth of fungi. The most prominent bacterial colony types were identified using a combination of methods that include recording characteristics of colony morphology; performing a Gram staining method and metabolic analyses using the Biolog system. Results from thirty-five AGI samples taken at the five stations through seven sampling events over four seasons indicate that there were significantly lower total bacterial concentrations in the samples collected from ambient stations as compared with the samples col...
16 citations
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TL;DR: Overall this study demonstrated that SIBS provides additional spectral information compared to existing instruments and capability to resolve spectrally integrated signals from relevant biological fluorophores could improve selectivity and thus enhance discrimination and classification strategies for real-time characterisation of bioaerosols from environmental sources.
16 citations