Topic
Bioaerosol
About: Bioaerosol is a research topic. Over the lifetime, 1347 publications have been published within this topic receiving 34791 citations.
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TL;DR: In this article, a matrix-assisted laser desorption/ionization mass spectrometer (MALDI) was proposed for the analysis of biological aerosols, which is capable of selecting only those aerosol particles that emit fluorescence when excited with 266 nm laser light, which differentiates the biological particles from virtually all other particles likely to be present in an aerosol.
Abstract: The development of an aerosol mass spectrometer for the analysis of biological aerosols is described. The working principles of the aerosol mass spectrometer are particle sizing, particle selection and particle analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI). The instrument has the capability of selecting only those aerosol particles that emit fluorescence when excited with 266 nm laser light, which differentiates the biological particles from virtually all other particles likely to be present in an aerosol. The implementation of a new ion source and delayed extraction has resulted in the capability of obtaining high-quality mass spectra of single bioaerosol particles. Isotopic resolution was obtained for a low-mass peptide. The sensitivity limit of the instrument was determined to be 1 zeptomole. The suitability of the aerosol mass spectrometer for the analysis of bacterial aerosol particles is demonstrated with an aerosol containing vegetative cells of the bacterium Escherichia coli when prepared off-line. The mass spectrum obtained has good resolution and covers a mass range up to 15 kDalton.
16 citations
Journal Article•
TL;DR: Coenen et al. as mentioned in this paper investigated the effect of exposure to low levels of bioaerosols on PEFmonitoring and antibody responses, and concluded that the changes in peak flow variability and the elevated concentrations of immunoglobulins may be used as indicators of sub-clinical effects of relatively low exposure to organic dust.
Abstract: To study the effect of exposure to low levels of bioaerosols on PEFmonitoring and antibody responses, five groups of waste collectors from different areas in Denmark were investigated. The five groups handled different kinds of waste using different kinds of organisation logistics and collection equipment. One group worked with a new, experimental system (“System 2000”), which involved source separation of waste into compostable and residual fractions. Waste collectors exposed to “high” (maximum of 72 EU/m) levels of endotoxins had significantly (p < 0.001) higher serum concentrations of IgG than waste collectors exposed to “low” levels of endotoxins. Working with “System 2000” resulted in significantly higher serum concentrations of IgG (p < 0.001) and IgA (p < 0.01). Peak flow variability appeared to be significantly affected by exposure to Aspergillus fumigatus. It is concluded that the changes in peak flow variability and the elevated concentrations of immunoglobulins may be used as indicators of sub-clinical effects of relatively low exposure to organic dust. Assessment of peak flow variability (or serum IgG concentrations) may be used in surveillance systems to prevent clinical symptoms caused by organic dust exposure. Address for correspondence: Gerard J. Coenen, National Institute of Occupational Health, Lersø Parkallé 105, DK-2100 Copenhagen Ø, Denmark.
16 citations
TL;DR: The study indicates significantly higher bacterial and fungal bioaerosols during the fair event and the proportion of antibiotic-resistant bacteria over the fair ground was significantly increased during-fair and was still higher in the after-fair period.
16 citations
TL;DR: A rapidly scanning lidar system, the Laser Cloud Mapper (LCM), was operated to determine and assess its sensitivity as well as its mapping capability for the remote characterization of airborne biological organisms.
15 citations
TL;DR: Total, viable and culturable bacteria in the air of various indoor and outdoor environments were further quantified and a descending order of total counts>viable counts>culturable counts was observed regardless of sampling location, highlighting the applicability of PMA-qPCR in field aerobiology.
15 citations