Topic
Bioaerosol
About: Bioaerosol is a research topic. Over the lifetime, 1347 publications have been published within this topic receiving 34791 citations.
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TL;DR: The data indicate that the amount of cell damage during bioaerosol sampling and the resulting release of DNA can be substantial and that this should be taken into account when analyzing bioaerOSol samples.
Abstract: We report here that stress experienced by bacteria due to aerosolization and air sampling can result in severe membrane impairment, leading to the release of DNA as free molecules. Escherichia coli and Bacillus atrophaeus bacteria were aerosolized and then either collected directly into liquid or collected using other collection media and then transferred into liquid. The amount of DNA released was quantified as the cell membrane damage index (ID), i.e., the number of 16S rRNA gene copies in the supernatant liquid relative to the total number in the bioaerosol sample. During aerosolization by a Collison nebulizer, the ID of E. coli and B. atrophaeus in the nebulizer suspension gradually increased during 60 min of continuous aerosolization. We found that the ID of bacteria during aerosolization was statistically significantly affected by the material of the Collison jar (glass > polycarbonate; P B. atrophaeus; P < 0.001). When E. coli was collected for 5 min by filtration, impaction, and impingement, its ID values were within the following ranges: 0.051 to 0.085, 0.16 to 0.37, and 0.068 to 0.23, respectively; when it was collected by electrostatic precipitation, the ID values (0.011 to 0.034) were significantly lower (P < 0.05) than those with other sampling methods. Air samples collected inside an equine facility for 2 h by filtration and impingement exhibited ID values in the range of 0.30 to 0.54. The data indicate that the amount of cell damage during bioaerosol sampling and the resulting release of DNA can be substantial and that this should be taken into account when analyzing bioaerosol samples.
69 citations
TL;DR: In this paper, the authors investigated the survivals of both lab-generated and environmental bioaerosols when exposed to the microwave irradiation (2450MHz) for ∼2min at different output powers (700, 385 and 119 W).
69 citations
TL;DR: In this paper, the authors examined the effect of dust storms on indoor and outdoor microbial air quality at a hospital on the western side of Iran (city of Ahvaz), which is notorious for being highly vulnerable to dust emissions.
69 citations
TL;DR: In this paper, the authors proposed an electrostatic precipitator with super-hydrophobic surface for bioaerosol sampling, which achieved very high sample concentration rates (up to 1 million and higher) and could be applied to detect low concentrations of bio-aerosols in various environments.
68 citations
TL;DR: Exposure was also affected by season of the year—the concentration of total microorganisms, culturable fungi, Aspergillus fumigatus and endotoxin was low in winter.
Abstract: Recent Danish studies on waste collectors' bioaerosol exposure are summarized. Generally the median exposure levels ranged from 105 to 106 cells m−3 (total microorganisms), 104 to 105 cfu m−3 (culturable fungi) and 103 to 104 cfu m−3 (culturable bacteria). The type of waste was a governing factor for exposure. Garden waste collectors frequently experienced concentrations exceeding 105 cfu m−3 for mesophilic fungi and 104 cfu m−3 for the thermophilic fungus Aspergillus fumigatus. Workers collecting compostable, mixed and sorted waste occasionally experienced similar concentrations of the fungal groups while workers collecting ‘bulky waste’ and paper had low exposure. Type of collection vehicle was identified as another governing factor for exposure. Vehicles loaded from the top (approximately 3 m above the ground) caused lower exposure (by a factor of 25) to fungi than vehicles loaded at the level or the breathing zone of the workers. Exposure was also affected by season of the year—the concentration of total microorganisms, culturable fungi, Aspergillus fumigatus and endotoxin was low in winter. Exposure to total microorganisms counted by microscopy was found to have a fairly high validity (Va) as an indicator of exposure to culturable fungi (Va = 1.45) or culturable bacteria (Va = 1.25). Likewise, dust may also be used as an indicator of exposure to total microorganisms (Va= 1.36), culturable fungi (Va= 1.31) and culturable bacteria (Va = 1.35).
68 citations