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Bioaerosol

About: Bioaerosol is a research topic. Over the lifetime, 1347 publications have been published within this topic receiving 34791 citations.


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Journal ArticleDOI
TL;DR: Experiments to investigate clothing release fraction (CRF), determined as the size-dependent ratio of released to deposited particulate matter in the diameter range 0.5-10 μm, confirmed that clothing can act as a vehicle for transporting airborne particles.
Abstract: Strong evidence suggests that clothing serves as a reservoir of chemical pollutants and particles, including bioaerosols, which may have health significance. However, little is known about the role that clothing may play as a transport vector for inhaled airborne particles. Here, we contribute toward bridging the knowledge gap by conducting experiments to investigate clothing release fraction (CRF), determined as the size-dependent ratio of released to deposited particulate matter in the diameter range 0.5-10 μm. In a fully controlled chamber with low background particle levels, we deployed a programmable robot to reproducibly quantify the size-dependent CRF as a function of motion type and intensity, dust loadings, and activity duration. On average, 0.3%-3% of deposited particles were subsequently released with fabric motion, confirming that clothing can act as a vehicle for transporting airborne particles. The CRF increased with the vigor of movement and with dust loading. Rubbing and shaking the fabric were more effective than fabric stretching in resuspending particles. We also found that most of the release happened quickly after the onset of the resuspension activity. Particle size substantially influenced the CRF, with larger particles exhibiting higher values.

40 citations

Journal ArticleDOI
TL;DR: A high-flow-rate impinger described here has many advantages compared with other bioaerosol samplers currently on the market: a potential for long sampling time, ahigh flow rate, a high sampling and retention efficiency, low costs, and applicability for diverse downstream microbiological and molecular analyses.
Abstract: The study of airborne bacteria relies on a sampling strategy that preserves their integrity and in situ physiological state, e.g. viability, cultivability, metabolic activity, and ice-nucleation activity. Because ambient air harbors low concentrations of bacteria, an effective bioaerosol sampler should have a high sampling efficiency and a high airflow. We characterize a high-flow-rate impinger with respect to particle collection and retention efficiencies in the range 0.5-3.0 μm, and we investigated its ability to preserve the physiological state of selected bacterial species and seawater bacterial community in comparison with four commercial bioaerosol samplers. The collection efficiency increased with particle size and the cutoff diameter was between 0.5 and 1 μm. During sampling periods of 120-300 min, the impinger retained the cultivability, metabolic activity, viability, and ice-nucleation activity of investigated bacteria. Field studies in semiurban, high-altitude, and polar environments included periods of low bacterial air concentrations, thus demonstrating the benefits of the impinger's high flow rate. In conclusion, the impinger described here has many advantages compared with other bioaerosol samplers currently on the market: a potential for long sampling time, a high flow rate, a high sampling and retention efficiency, low costs, and applicability for diverse downstream microbiological and molecular analyses.

40 citations

Journal ArticleDOI
TL;DR: Two prototype personal bioaerosol samplers in which size-selective substrates are adapted for use in existing designs of personal inhalable sampler offer an effective and low-cost solution to personal monitoring of bioaerOSol exposures in the workplace.
Abstract: Health-related monitoring of bioaerosol exposures in the workplace should ideally be carried out using size-selective personal samplers that separate the aerosol into biologically relevant size fractions and allow both quantification and identification of the microorganisms present in each fraction. As a first stage in the development of personal bioaerosol samplers a number of collection substrates were assessed for their ability to maintain the viability of the collected microorganisms, so that subsequent culturing and species identification may be carried out. The substrates were tested with bioaerosols of varying robustness, consisting of Saccharomyces cerevisiae cells, Escherichia coli cells, and Penicillium expansum spores, aerosolized under controlled environmental conditions. The survival of microorganisms on each test substrate, assessed on the basis of the culturable fractions of cells recovered, was compared with that of microorganisms collected in a reference glass cyclone sampler. These bioef...

40 citations

Journal ArticleDOI
TL;DR: Air filtration and liquid impingement for bioaerosol sampling across a range of environmental conditions are evaluated and a resource is provided that enables molecular ecologists to select the most appropriate sampling strategy for their specific research question.
Abstract: Bioaerosols (or biogenic aerosols) have largely been overlooked by molecular ecologists. However, this is rapidly changing as bioaerosols play key roles in public health, environmental chemistry and the dispersal ecology of microbes. Due to the low environmental concentrations of bioaerosols, collecting sufficient biomass for molecular methods is challenging. Currently, no standardized methods for bioaerosol collection for molecular ecology research exist. Each study requires a process of optimization, which greatly slows the advance of bioaerosol science. Here, we evaluated air filtration and liquid impingement for bioaerosol sampling across a range of environmental conditions. We also investigated the effect of sampling matrices, sample concentration strategies and sampling duration on DNA yield. Air filtration using polycarbonate filters gave the highest recovery, but due to the faster sampling rates possible with impingement, we recommend this method for fine -scale temporal/spatial ecological studies. To prevent bias for the recovery of Gram-positive bacteria, we found that the matrix for impingement should be phosphate-buffered saline. The optimal method for bioaerosol concentration from the liquid matrix was centrifugation. However, we also present a method using syringe filters for rapid in-field recovery of bioaerosols from impingement samples, without compromising microbial diversity for high -throughput sequencing approaches. Finally, we provide a resource that enables molecular ecologists to select the most appropriate sampling strategy for their specific research question.

40 citations

Journal ArticleDOI
TL;DR: Preliminary studies using this platform have revealed interactions between PM2.5 and the enveloped bacteriophage Φ6 that reduce infectivity of the bacteriophile by 44% compared to a control exposed only to HEPA-filtered air.

40 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023133
2022235
202195
202094
201989
201871