Topic
Bioaerosol
About: Bioaerosol is a research topic. Over the lifetime, 1347 publications have been published within this topic receiving 34791 citations.
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TL;DR: The overall goal of the study was to develop methods for obtaining mass spectra with minimal reagents and treatment steps for potential use in remote collection and analysis systems.
Abstract: A method was developed for collection and analysis of bioaerosols by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry using a modified Andersen N6 bioaerosol collector. The overall goal of the study was to develop methods for obtaining mass spectra with minimal reagents and treatment steps for potential use in remote collection and analysis systems. Test bioaerosol particles were generated from a nebulized E. coli bacterial suspension and collected on MALDI targets placed in an Andersen N6 single-stage aerosol impactor. The bioaerosols were mixed with matrix either by deposition on a bare target with the matrix solution added later, or by deposition on a target pre-coated with matrix. The matrix compounds α-cyano-4-hydroxycinnamic acid (CHCA) and sinapic acid (SA) were tested and the SA matrix was found to give the best results in number of peaks, resolution, and signal-to-noise ratio. Deposition of bioaerosol particles onto the matrix pre-coated target did not produce signal in the m/z region above 1000, but the signal could be recovered with the addition of a 1:1 (v/v) acetonitrile/water solvent. Addition of solvent by pipette to the pre-coated targets after particle deposition recovered signal comparable to the dried-droplet sample preparations, whereas solvent sprayed into the impactor recovered fewer peaks. Deposition on pre-coated targets with post-collection solvent addition was superior to deposition on bare target followed by post-collection addition of matrix solution. Copyright © 2005 John Wiley & Sons, Ltd.
37 citations
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TL;DR: Under the given conditions the results of both samplers are approximately comparable, and the spectrum of thermotolerat fungal genera and Aspergillus species showed only minor differences.
37 citations
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TL;DR: In this article, the concentration of culturable bacteria and fungi aerosols in indoor and outdoor air of twelve kindergartens in Rasht, Iran was determined, and the mean concentrations of fungi versus bacteria in indoor air were 7.6 and 7.5 CFU/plate/hr, respectively.
37 citations
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TL;DR: A method for evaluating the contribution of overall PBAPs to the mass concentration of atmospheric particulate matter (PM), based on PM sampling on polycarbonate filters, staining of the collected particles with propidium iodide, observation at epifluorescence microscope and calculation of the bioaerosol mass using a digital image analysis software is developed.
37 citations
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TL;DR: Most workstations respected the suggested minimum values for fluid mist and showed low concentrations of airborne endotoxin, culturable microorganisms and inhalable dust despite fluid contamination, even when air exchange rates were below the recommendations.
Abstract: Respiratory problems are observed in machinists using soluble metalworking fluid (MWF). Evidences suggest that these problems could be related to the aerosolized microorganisms and their byproducts from MWF. To establish MWF aerosol exposure thresholds and to better understand their effect on human health, these aerosols must be fully characterized. This article evaluates airborne microorganisms and aerosols from soluble MWF in the working environment. Air quality parameters (endotoxin levels, culturable airborne microorganisms, fluid mist, inhalable dust and air exchange rates) were evaluated at 44 sites, in 25 shops in Quebec, Canada. Microorganism concentrations were also measured in MWF. Culturable airborne bacteria concentrations were low, ranging from 1.2 × 101 to 1.5 × 103 CFU (colony forming units) m− 3, even for metalworking fluid highly contaminated by bacteria (up to 2.4 × 109 CFU mL− 1). Inhalable dust varied between < 0.1 to 2.6 mg m− 3, while air exchange rates were mostly below the standard...
37 citations