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Bioaerosol

About: Bioaerosol is a research topic. Over the lifetime, 1347 publications have been published within this topic receiving 34791 citations.


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Journal ArticleDOI
TL;DR: Aerobiological studies in commuting trains in northern Taiwan were carried out from August, 2007 until July, 2008 as mentioned in this paper, where two six-stage cascade impactors of 400 orifices were used to collect viable bacteria and fungi.

28 citations

Journal ArticleDOI
TL;DR: In this article, a non-culture-dependent technique, known as epifluorescence microscopy (EM), with several fluorochromes was used to characterize and quantify bioaerosols.

27 citations

Journal ArticleDOI
TL;DR: The results provide new bioaerosol concentration data in a composting facility and suggest that the filtration sampling method might be a useful tool for exposure measurements in that occupational environment.
Abstract: Bioaerosol concentrations were investigated in a totally indoor composting facility processing fermentable household and green wastes to assess their variability. Stationary samples were collected by filtration close to specific composting operations and then were analysed for cultivable mesophilic bacteria, thermophilic bacteria, mesophilic fungi, thermophilic fungi, endotoxins and total airborne bacteria (DAPI-staining). Indoor concentrations exceeded the background levels, between 500 and 5400 EU m−3 for endotoxins, 104 and 106 CFU m−3 for cultivable bacteria and generally below 105 CFU m−3 for airborne cultivable fungi. No significant (p > 0.05) differences were observed between the indoor composting operations. Successive 30 minute bioaerosol samples were collected to investigate the variation of cultivable mesophilic microorganisms over the work shift. Concentrations of mesophilic bacteria and fungi varied up to 1 log unit depending on the time at which they were collected in the day. Total airborne particles, counted using an optical particle counter, were present at up to 108 particles m−3 and several concentration peaks were noted. Values for total airborne bacteria were roughly 70-fold higher than cultivable bacteria. These results raise the question of the sampling strategy (duration of sampling; number of samples to be collected) used in similar studies. They provide new bioaerosol concentration data in a composting facility and suggest that the filtration sampling method might be a useful tool for exposure measurements in that occupational environment.

27 citations

Journal ArticleDOI
TL;DR: The results indicate that bioaerosol emitted by this municipal facility is the source of hemolytic bacteria, as well as of pathogenic bacteria (Pseudomonas aeruginosa and Bacillus subtilis) and the need for regular monitoring of microbiological tests in their vicinity is emphasized.
Abstract: The present research was aimed at assessing the quality of air and soil on the premises and in the vicinity of the municipal landfill sites in Torun with regard to the presence of pathogenic bacteria, potentially dangerous to humans. Air samples (the impaction method using a MAS-100 impactor) and soil samples were collected from seven sampling sites including the operating and closed landfill cells, sampling sites located near leachate ponds, and sampling sites located outside the above premises. The research also involved assessing microbial air contamination in three indoor spaces on the premises of the landfill sites. Microbial tests involved the determination of the number of culturable mesophilic, mannitol-positive, and α- and β-hemolytic bacteria in the air, determination of the number of coliform bacteria, spore-forming Clostridium perfringens in soil, and the presence of Salmonella in soil. The results indicate that bioaerosol emitted by this municipal facility is the source of hemolytic bacteria (≤300 CFU m−3 of air), as well as of pathogenic bacteria (Pseudomonas aeruginosa and Bacillus subtilis). The highest risk of exposure to biological agents was determined in the sorting facility. Over sixty percent of air samples in this sampling site presented high pollution degree with mesophilic bacteria (500–2000 CFU m−3 of air) and over one fourth of air samples presented very high pollution degree (>2000 CFU m−3 of air). Indoor air in other rooms was considered highly/moderately contaminated (100–2000 CFU m−3 of air). The highest risk related to the presence of Salmonella, Clostridium perfringens, and coliform bacteria in soil was determined at the operating landfill cell and near the leachate pond of the closed landfill cell. At the operating landfill cell the total coli ranged from 4–1226 MPN g−1 of dry mass of soil and Clostridium perfringens ranged from

27 citations

Journal ArticleDOI
TL;DR: This work discusses sampling and analysis techniques that can be integrated with molecular detection procedures to properly assess the aerosolized cellular material of interest and investigates the feasibility of identifying humans via bioaerosols.
Abstract: The purpose of this review is to investigate the feasibility of bioaerosol fingerprinting based on current understanding of cellular debris (with emphasis on human-emitted particulates) in aerosols and arguments regarding sampling, sensitivity, separations, and detection schemes. Target aerosol particles include cellular material and proteins emitted by humans, animals, and plants and can be regarded as information-rich packets that carry biochemical information specific to the living organisms present where the sample is collected. In this work we discuss sampling and analysis techniques that can be integrated with molecular (e.g. protein)-detection procedures to properly assess the aerosolized cellular material of interest. Developing a detailed understanding of bioaerosol molecular profiles in different environments suggests exciting possibilities of bioaerosol analysis with applications ranging from military defense to medical diagnosis and wildlife identification

27 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023133
2022235
202195
202094
201989
201871