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Biomolecule

About: Biomolecule is a research topic. Over the lifetime, 1474 publications have been published within this topic receiving 32922 citations. The topic is also known as: biological molecule & natural compound.


Papers
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Journal ArticleDOI
TL;DR: A facile two-step aqueous approach to immobilization of biomolecules onto surfaces is reported, which exploits the latent reactivity of the biomimetic polymer thin film towards nucleophiles, is unaffected by water, and allows for discrimination betweenucleophiles on the basis of pKa.
Abstract: Immobilization of biomolecules onto surfaces is important in many of the biological and physical sciences, including cell and molecular biology, analytical chemistry, and in applied and interdisciplinary fields such as medical diagnostics, tissue engineering, and bioprocess engineering.[1-4] Strategies for biomolecule immobilization onto surfaces generally exploit either noncovalent or covalent reactions. Noncovalent methods allow reversible immobilization of biomolecules under specific conditions, and include physical adsorption and affinity immobilization. Some widely adapted examples are (strep)avidin-biotin, nitriloacetic acid (NTA)-histidine, and DNA-DNA interactions.[5-8] In contrast, covalent immobilization of molecules onto surfaces typically relies on conjugation reactions between ‘active’ functional groups, such as N-hydroxysuccinimide (NHS)[9] or maleimide,[10] and companion target moieties, such as amines and sulfhydryls. For reactions involving biomolecules performed in aqueous solvents, susceptibility of NHS, maleimide, and other activating groups to hydrolysis during storage and reaction can lead to low efficiency of surface bioconjugation.[11,12] In this study, we report a facile two-step aqueous approach to immobilization of biomolecules onto surfaces. The approach involves simple dip-coating of a biomimetic polymer thin film onto a substrate, followed by conjugation of biomolecules to the biomimetic polymer film. The method exploits the latent reactivity of the biomimetic polymer thin film towards nucleophiles, is unaffected by water, and allows for discrimination between nucleophiles on the basis of pKa.

1,324 citations

Journal ArticleDOI
TL;DR: The construction of controlled architectures based on spatially segregated multilayers, exhibiting complementary biological activities is described, and the attachment of biomolecules to biotinylated polymers through affinity interactions based on avidin-biotin bridge is presented.

731 citations

PatentDOI
TL;DR: In this paper, compositions and methods for increasing the intrinsic fluorescence intensity of biomolecules and low quantum yield fluorophores are described. And methods for the identification of nucleic acids are also provided.

706 citations

Journal ArticleDOI
TL;DR: It is demonstrated with suitable examples that monolayer design plays a key role in controlling the performance of these SAM based biosensors, irrespective of the immobilisation strategy and sensing mechanism.

704 citations

Patent
30 Jul 1999
TL;DR: In this paper, a device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a surface density which allows each molecule to be individually resolved, e.g. by optical microscopy.
Abstract: A device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a surface density which allows each molecule to be individually resolved, e.g. by optical microscopy. Therefore, the arrays of the present invention consist of single molecules are more spatially distinct than the arrays of the prior art.

564 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023958
20221,980
202191
202055
201983
201866