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Bioprocess

About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.


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Journal ArticleDOI
TL;DR: Embedded within the presented cultivation workflow, the 1 mL MTP‐based parallelized cultivation system seems to be a suitable screening tool for filamentous and industrial relevant organisms like Streptomyces and can contribute to widen the field of application for these organisms and facilitate screening and early‐stage bioprocess development.
Abstract: Filamentous organisms of the genus Streptomyces play an important role in industrial production processes, due to their extensive secondary metabolism variability, as well as their ability to secrete efficiently large amounts of (heterologous) proteins. While genetic engineering tools are available to rapidly build up large strain libraries, the subsequent strain screening and bioprocess development still constitutes a bottleneck. This is due to the lack of reliable parallelized and accelerated cultivation techniques for morphologically challenging organisms. To address this challenge, we developed an integrated cultivation workflow for Streptomyces lividans based on a parallelized shaken 48-well microtiter-plate (MTP) cultivation device. In a first step, a feasible pre-culture method was identified and validated, revealing high comparability in subsequent main cultivations (coefficient of variation of 1.1% for in-plate replicates and 3.2% between different pre-cultures). When validating the growth performance in 1 mL MTP cultivation against an established 1,000 mL lab-scale cultivation system, highly comparable cultivation patterns were found for online (pH, dissolved oxygen), as well as for offline derived parameters (glucose uptake, cell-dry-weight, and pellet size). Additionally, the two cultivation regimes were compared with respect to transcriptional and protein secretion activity of Streptomyces, showing overall good comparability with minor, but well explainable discrepancies, most probably caused by different energy dissipation (shaking vs. stirring) and adaption effects due to different illumination conditions. Embedded within the presented cultivation workflow, the 1 mL MTP-based parallelized cultivation system seems to be a suitable screening tool for filamentous and industrial relevant organisms like Streptomyces. This can contribute to widen the field of application for these organisms and facilitate screening and early-stage bioprocess development. Biotechnol. Bioeng. 2017;114: 2011-2022. © 2017 Wiley Periodicals, Inc.

27 citations

Book ChapterDOI
TL;DR: The monitoring of apoptosis induction is also becoming more and more important in bioprocess control to increase volumetric productivity by extending biop rocess duration.
Abstract: The accurate determination of cell growth and viability is pivotal to monitoring a bioprocess Direct methods to determine the cell growth and/or viability in a bioprocess include microscopic counting, electronic particle counting, image analysis, in situ biomass monitoring, and dieletrophoretic cytometry These methods work most simply when a fixed volume sample can be taken from a suspension culture Manual microscopic counting is laborious but affords the advantage of allowing cell viability to be determined if a suitable dye is included Electronic particle counting is a rapid total cell count method for replicate samples, but some data distortion may occur if the sample has significant cell debris or cell aggregates Image analysis based on the use of digital camera images acquired through a microscope has advanced rapidly with the availability of several commercially available software packages replacing manual microscopic counting and viability determination Biomass probes detect cells by their dielectric properties or their internal concentration of NADH and can be used as a continuous monitor of the progress of a culture While the monitoring of cell growth and viability is an integral part of a bioprocess, the monitoring of apoptosis induction is also becoming more and more important in bioprocess control to increase volumetric productivity by extending bioprocess duration Different fluorescent assays allow for the detection of apoptotic characteristics in a cell sampleIndirect methods of cell determination involve the chemical analysis of a culture component or a measure of metabolic activity These methods are most useful when it is difficult to obtain intact cell samples However, the relationship between these parameters and the cell number may not be linear through the phases of a cell culture The determination of nucleic acid (DNA) or total protein can be used as an estimate of biomass, while the depletion of glucose from the media can be used as an estimate of cellular activity The state of cellular viability may be measured by the release of an enzyme such as lactate dehydrogenase or more directly from the intracellular adenylate energy charge from cell lysates Alternatively, radioactive techniques may be used for an accurate determination of cellular protein synthesis

27 citations

Book ChapterDOI
TL;DR: This chapter addresses the update progress in bioprocess engineering with an overview of the theory of multi-scale analysis for fermentation process and examples of scale-up practice combining microbial physiological parameters with bioreactor fluid dynamics.
Abstract: This chapter addresses the update progress in bioprocess engineering. In addition to an overview of the theory of multi-scale analysis for fermentation process, examples of scale-up practice combining microbial physiological parameters with bioreactor fluid dynamics are also described. Furthermore, the methodology for process optimization and bioreactor scale-up by integrating fluid dynamics with biokinetics is highlighted. In addition to a short review of the heterogeneous environment in large-scale bioreactor and its effect, a scale-down strategy for investigating this issue is addressed. Mathematical models and simulation methodology for integrating flow field in the reactor and microbial kinetics response are described. Finally, a comprehensive discussion on the advantages and challenges of the model-driven scale-up method is given at the end of this chapter.

27 citations

Journal ArticleDOI
TL;DR: In this paper, a consolidated bioprocess integrating enzyme production, saccharification and fermentation was applied for bioethanol production, which avoided enzyme preparation, saved much water/energy consumption and reduced equipment investment.

27 citations

Journal ArticleDOI
TL;DR: The optimisation of a mediated amperometric glucose biosensor designed for in situ bioprocess monitoring leading to improved stability (4 days of continuous use) and extended working range (up to 20 g 1−1) is described.

27 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023331
2022785
2021165
2020153
2019159
2018127