Topic
Bioprocess
About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.
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TL;DR: The utilisation of tryptophan fluorescence as an indirect biomass measurement for the yeast Pichia pastoris, an excellent host system for the production of heterologous proteins, is presented.
Abstract: The utilisation of tryptophan fluorescence as an indirect biomass measurement for the yeast Pichia pastoris, an excellent host system for the production of heterologous proteins, is presented. Direct fluorescence measurements for cell densities above 3 g dm−3 presented important interferences due to inner filter effects. To overcome this drawback, a dilution protocol is provided which allows the quenching of the emission signal caused by solid particles to be controlled. The measured tryptophan fluorescence intensities were used to estimate biomass concentration during a P pastoris batch bioprocess growing either on glycerol or methanol. The best measurement model tested was based on the application of a Luedeking–Piret-based equation to fluorometric measurements. Thus, a linear relationship between the specific fluorescence evolution rate and specific growth rate was applied. The mean absolute relative prediction error (MARE) for biomass concentration was about 6%. Copyright © 2005 Society of Chemical Industry
24 citations
TL;DR: Current techno-economic studies at process scale have demonstrated the absolute need for significant improvements in both microorganism and process technology, for these processes to become competitive.
24 citations
TL;DR: In this article, the feasibility of applying emerging processing technologies such as moderate electric field (MEF) and ohmic heating to improve this bioprocess is explored, and the current status, potential benefits, mechanisms, and limitations of innovative MEF-and ohmic-assisted fermentation are demonstrated.
Abstract: Fermentation is an important bioprocess in food production and its improvements can bring profits to the food industry. Therefore, researchers are exploring the feasibility of applying emerging processing technologies such as moderate electric field (MEF) and ohmic heating to improve this bioprocess. This study demonstrated the current status, potential benefits, mechanisms, and limitations of innovative MEF- and ohmic-assisted fermentation. Research showed that these techniques can positively affect Lactobacillus, Streptococcus, and Saccharomyces fermentations that are involved in the production of bakery (e.g., leavened breads), dairy (e.g., yogurt), and alcoholic products. Also, volumetric ohmic heating can accelerate fermentation by providing optimum fermentation temperatures quickly. MEF-induced stress-response conditions can affect microbial metabolism and fermentation products. Besides, electrical fields may affect the fermentation process by altering the substrate such as releasing its micronutrients. These approaches can be considered prospective industrial fermentation tools. Further economic studies and in-depth research on their effects on fermentation by-products are expected in the near future.
24 citations
TL;DR: It is demonstrated that the two-stage bioprocess using the strain Enop56 was a promising strategy to produce FOSs on an industrial scale.
24 citations
Book•
01 Nov 1992
TL;DR: In this article, the authors present principles of bioreactor modelling and the ISM simulation language for using the ISIM language for modeling bioreactors in the context of biological kinetics and mass transfer diffusion.
Abstract: Part 1 Principles of bioreactor modelling: modelling principles basic bioreactor design biological kinetics bioreactor modelling mass transfer diffusion and biological reaction in immobilized biocatalyst systems automatic bioprocess control fundamentals. Part 2 Dynamics bioprocess simulation examples and the ISM simulation language: introductory examples batch reactors fed batch reactors continuous reactors oxygen uptake systems controlled reactors diffusion systems multi-organism and structured models instructions for using the ISIM language.
24 citations