Bioprocess

About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.

Universal label-free in-process quantification of influenza virus-like particles.

Abstract: Virus-like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion These analytical technologies are time-consuming, cumbersome, and not supportive of efficient downstream process development and monitoring Hereby we report a label-free tool that uses Biolayer interferometry (BLI) technology applied on an Octet platform to quantify Influenza VLPs at all stages of bioprocess Human (α2,6-linked sialic acid) and avian (α2,3-linked sialic acid) biotinylated receptors associated with streptavidin biosensors were used, to quantify hemagglutinin content in several mono- and multivalent Influenza VLPs The applied method was able to quantify hemagglutinin from crude samples up to final bioprocessing VLP product BLI technology confirmed its value as a high throughput analytical tool with high sensitivity and improved detection limits compared to traditional methods This simple and fast method allowed for real-time results, which are crucial for in-line monitoring of downstream processing, improving process development, control and optimization

The microfluidic bioreactor for a new era of bioprocess development

Abstract: The BioLector® Pro system from m2p‐labs GmbH uses microtiter plates (MTPs) with an integrated microfluidic chip. By using microfluidic technology, the system can successfully carry out small‐scale fed‐batch cultivations. Working volumes of 0.8–1.5 mL are used to conduct cultivations as they were only as yet possible in lab fermenters. The measurements of biomass, fluorescence, pH and dissolved oxygen are performed by non‐invasive optical methods. The control of pH and feeding rates are realized by micro‐valves and micro‐channels. For the first time, these unique microfluidic components achieve continuous feeding and pH control on an MTP format. Altogether, 32 bioreactor wells and 16 reservoir wells are placed on one plate. That means 32 fed‐batch cultivations can be run in parallel, completely automated, with extensive data output.

Recycling of lignocellulosic waste materials to produce high-value products: single cell oil and xylitol.

Abstract: Application of microorganisms with ability of using environmental wastes such as lignocellulosic materials for converting them to high-value products is important from economical point of view. Valuable products such as single cell oil (SCO) have a high potential to be used in various industrial fields including biodiesel production. Other important products are polyalcohols like xylitol, which are applicable in the food and pharmaceutical industries. In this study, Rhodotorula yeast was isolated from the leaves of Benjamin. Afterward, SCO production was evaluated in a nitrogen-limited medium, and the obtained oil was analyzed by gas chromatography–mass spectrometery. Moreover, xylitol was produced in a media containing xylose and analyzed by high-performance liquid chromatography technique. The yeast strain was identified using polymerase chain reaction method. SCO and xylitol production was also evaluated in a medium containing lignocellulosic materials and other forestry residues. This strain produced SCO and dry biomass of 9.7 and 16.14 g/L, respectively. In addition, in a medium with 140 g/L of xylose, xylitol production was found to be 49.28 g/L. Among waste materials, lipid content of 49 % on grass hydrolysate was found to be incredible. The obtained strain was identified as Rhodotorula mucilaginosa. The results of this study show that by the isolation of yeast with the potential of making use of waste materials, high-value products can be obtained. Therefore, it can be concluded that the mentioned bioprocess has not only environmental benefits but also is very important from economical view.

Propionic Acid: Method of Production, Current State and Perspectives.

Abstract: During the past years, there has been a growing interest in the bioproduction of propionic acid by Propionibacterium. One of the major limitations of the existing models lies in their low productivity yield. Hence, many strategies have been proposed in order to circumvent this obstacle. This article provides a comprehensive synthesis and review of important biotechnological aspects of propionic acid production as a common ingredient in food and biotechnology industries. We first discuss some of the most important production processes, mainly focusing on biological production. Then, we provide a summary of important propionic acid producers, including Propionibacterium freudenreichii and Propionibacterium acidipropionici, as well as a wide range of reported growth/production media. Furthermore, we describe bioprocess variables that can have impact on the production yield. Finally, we propose methods for the extraction and analysis of propionic acid and put forward strategies for overcoming the limitations of competitive microbial production from the economical point of view. Several factors influence the propionic acid concentration and productivity such as culture conditions, type and bioreactor scale; however, the pH value and temperature are the most important ones. Given that there are many reports about propionic acid production from glucose, whey permeate, glycerol, lactic acid, hemicelluloses, hydrolyzed corn meal, lactose, sugarcane molasses and enzymatically hydrolyzed whole wheat flour, only few review articles evaluate biotechnological aspects, i.e. bioprocess variables.