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Bioprocess

About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.


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Journal ArticleDOI
TL;DR: This case study demonstrates that it is possible to produce acceptably pure peptide for potential commodity applications using common scaleable bioprocess unit operations and is shown that BMD is a powerful strategy that can be deployed to reduce bioseparation complexity.
Abstract: The efficient expression and purification of an interfacially active peptide (mLac21) was achieved by using bioprocess-centered molecular design (BMD), wherein key bioprocess considerations are addressed during the initial molecular biology work. The 21 amino acid mLac21 peptide sequence is derived from the lac repressor protein and is shown to have high affinity for the oil-water interface, causing a substantial reduction in interfacial tension following adsorption. The DNA coding for the peptide sequence was cloned into a modified pET-31(b) vector to permit the expression of mLac21 as a fusion to ketosteroid isomerase (KSI). Rational iterative molecular design, taking into account the need for a scaleable bioprocess flowsheet, led to a simple and efficient bioprocess yielding mLac21 at 86% purity following ion exchange chromatography (and >98% following chromatographic polishing). This case study demonstrates that it is possible to produce acceptably pure peptide for potential commodity applications using common scaleable bioprocess unit operations. Moreover, it is shown that BMD is a powerful strategy that can be deployed to reduce bioseparation complexity. (C) 2004 Wiley Periodicals, Inc.

18 citations

Journal ArticleDOI
Daniel Koß1
TL;DR: In a recent review as discussed by the authors , a wide range of activities currently on-going in bioprocess intensification, focusing on upstream, bioreactor/fermentation and downstream separation steps.
Abstract: With the current pressing need to rise to the ambition of net zero targets to mitigate carbon emissions and climate change impacts, sustainable processing has never been more critical. Bioprocessing has all the desirable attributes to respond to the sustainable processing challenge: use of cheap, renewable resources, nature-inspired, highly selective biocatalysts operating optimally under mild conditions and reduced energy consumption/carbon footprint. With bioprocessing productivity being far from ideal to meet the large-scale need for food, drugs, biofuels and bio-based chemicals, there has been tremendous interest of late in developing intensified bioprocesses, with significant advancement achieved in tailoring and utilising the technologies in the toolbox traditionally applied in chemical process intensification. This review highlights the wide range of activities currently on-going in bioprocess intensification, focusing on upstream, bioreactor/fermentation and downstream separation steps. Great strides have been made in biocatalyst engineering and high density cell immobilisation for significant productivity enhancement, which, in conjunction with elegant process innovations such as novel bioreactor technologies and in-situ product separations, are enabling bioprocesses to become more competitive than ever before. The future prospects of bioprocess intensification are promising but there are still challenges that need to be overcome to fully exploit this technology.

18 citations

01 Jan 2008
TL;DR: An alternative strategy for lowering production costs that has been proposed is to develop transgenic plants that produce PHAs, which is considerably cheaper because the PHAs production from plants does not require expensive fermentation equip- ment and processing facilities.
Abstract: Currently, the polyhydroxybutyrate (PHB) copolymer, polyhydroxy-co-valerate (PHBV) is being produced commer- cially using a two-stage glucose / propionate fed batch fermentation process using Ralstonia eutropha. The economics of the manufacturing process are still a major barrier to the widespread use of polyhydroxyalkanoates (PHAs) and intensive efforts are being made to reduce the cost of production by means of bioprocess design and metabolic engineering of production strains. However, despite these improvements, the production costs are still high compared to petroleum-derived commodity plastics. An alternative strategy for lowering production costs that has been proposed is to develop transgenic plants that produce PHAs. This strategy is considerably cheaper because the PHAs production from plants does not require expensive fermentation equip- ment and processing facilities.

18 citations

Journal ArticleDOI
TL;DR: A rational approach for characterising the BC production process using Design of Experiment (DoE) methodology finds that the carbon source used for bacterial growth significantly impacts the interplay between the process variables and affects the desired outcomes.

18 citations

Journal ArticleDOI
TL;DR: In this article, the authors developed the fermentation conditions for enhanced Bacillus subtilis EPAH18 PHB biosynthesis and evaluated the economics of the bioprocess by process simulation.

18 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023331
2022785
2021165
2020153
2019159
2018127