Bioprocess

About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.

Electrochemical biosensors for food bioprocess monitoring

Abstract: During the last years, the use of biosensors in food analysis has gained considerable interest. These analytical devices provide highly selective, sensitive and cost-effective detection of cells, chemical or biochemical compounds that are relevant for food industry. In special, such sensors can be employed to control and monitoring biotechnological processes for food production and transformation. This review provides an overview on the state-of-the-art of electrochemical biosensors with potential use in food bioprocess technology, focusing on those with potential application in fermentation productions.

Nanotechnology and chemical engineering as a tool to bioprocess microalgae for its applications in therapeutics and bioresource management

Abstract: Microalgae are a dynamic biological resource with various biotechnological applications. During recent times, the scope of this application has expanded to include: nutritional health foods, pharmaceuticals, agricultural and industrial products, environmental remediation and bioenergy production. At the same time, the methods and technologies to bioprocess microalgae for the intended applications have also evolved. However, there are still significant developments needed to reach the full potential of microalgae. The presented review discusses current methodologies to improve the effectiveness of algal feedstocks by bioprocessing them innovatively with cost-effective and environmentally sustainable techniques for their applications in therapeutics and bioresource management. The first section discusses the diversity of microalgae and its applications. In following sections, bioprocessing microalgae for their applications in therapeutics focusing on the efficacy of algae-mediated metallic nanoparticles against microbial infections and cancer is discussed. In addition, a discussion on bioresource management to produce value-added products for bioenergy and bioresource conservation elaborated the potential of microalgae as a biological reservoir to resolve the energy crisis for the modern world.

Power and limitations of model based bioprocess optimization

Abstract: Since the last few decades the application of system theory concepts and modern model based control techniques to the optimization of biotechnological processes has received a lot of attention. This represents a challenging field of research for the following reasons. First, the dynamics of bioprocesses are determined by the non-linear behavior of living micro-organisms. Therefore, knowledge of reaction kinetics is most often only partial, which represents a serious limitation for mathematical modeling. In addition, bioprocess characteristics are time-varying, which requires on-line adaptation of the model parameters or even the model structure. Second, until now the number of on-line measurement systems is very small, while the measured quantities are most often only indirectly related to the important biological state variables. This inhibits the on-line validation of complex model structures. Therefore, in the past bioprocess optimization used no model at all. However, an adequate mathematical bioprocess description is the basic ingredient for the development of model based control strategies. As a case study, we consider in this paper the optimization of fed-batch fermentation processes involving one limiting substrate for biomass growth and product synthesis, with respect to the volumetric feed rate of this substrate. Three examples illustrate that feed rate profiles which are optimal in the sense of process performance, can contribute at the same time to solving model structure selection and parameter estimation problems as well.

Production of nisin and lactic acid from corn stover through simultaneous saccharification and fermentation

Abstract: The aim of this work was to demonstrate the feasibility of nisin and lactic acid production from corn stover through simultaneous saccharification and fermentation using Lactococcus lactis subsp. lactis ATCC 11454. The optimum conditions for nisin and lactic acid production were cellulose concentration of 30 g/L, a cellulase loading of 25 FPU/g cellulose and an initial pH of 6.0 in a 10 g/L yeast extract and 10 g/L peptone broth. The optimized bioprocess achieved nisin titer of 1635 IU/mL and lactic acid concentration of 15.2 g/L. The results indicated that simultaneous saccharification and fermentation with corn stover is a promising bioprocess for the economic production of nisin and lactic acid simultaneously.