Topic
Bioprocess
About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: In this paper , an overview of various cost-effective and greener enzymatic preparation strategies, mechanism of cellulose deconstruction and reconstruction, as well as the factors affecting bioprocesses is presented.
15 citations
TL;DR: Deciphering technical microbial ecology reveals differences and similarities of natural and technical systems objectives, which are discussed in this review in view of biocatalyst and process design and engineering strategies.
15 citations
TL;DR: Diagnostic analysis is the most important application for biosensing flow injection analysis, but other applications include bioprocess monitoring, analysis of food and agricultural products, as well as environmental analysis.
15 citations
TL;DR: The design criteria for the development of this web-based DownStream Process Design environment is described and it gives an impression of the use of the design environment in a course for first-year students.
Abstract: A bioprocess engineer should have at least a set of basic design skills. Bioprocess design is a complex cognitive skill, which should be trained in every year of an academic Bioprocess-Engineering curriculum. However, there is little existing learning material to support the initial training of design skills early in the curriculum. For this reason a web-based DownStream Process Design environment has been developed, called DSPD. This article describes the design criteria for the development of this design environment. It describes the design environment itself and it gives an impression of the use of the design environment in a course for first-year students.
15 citations
TL;DR: These findings clearly illustrate the underrated impact of room light after only short periods of exposure, occurring accidentally or knowingly during bioprocess development and scale‐ up.
Abstract: Background Many vital components in bioprocess media are prone to photo-conversion or photo-degradation upon exposure to ambient light, with severe negative consequences for biomass yield and overall productivity. However, there is only limited awareness of light irradiation as a potential risk factor when working in transparent glass bioreactors, storage vessels or disposable bag systems. The chemical complexity of most media renders a root-cause analysis difficult. This study investigated in a novel, holistic approach how light-induced changes in media composition relate to alterations in radical burden, cell physiology, morphology, and product formation in industrial Chinese hamster ovary (CHO) bioprocesses. Results Two media formulations from proprietary and commercial sources were tested in a pre-hoc light exposure scenario prior to cultivation. Using fluorescence excitation/emission (EEM) matrix spectroscopy, a photo-sensitization of riboflavin was identified as a likely cause for drastically decreased IgG titers (up to -80%) and specific growth rates (-50% to -90%). Up to three-fold higher radical levels were observed in photo-degraded medium. On the biological side, this resulted in significant changes in cell morphology and aberrations in the normal IgG biosynthesis/secretion pathway. Conclusion These findings clearly illustrate the underrated impact of room light after only short periods of exposure, occurring accidentally or knowingly during bioprocess development and scale- up. The detrimental effects, which may share a common mechanistic cause at the molecular level, correlate well with changes in spectroscopic properties. This offers new perspectives for online monitoring concepts, and improved detectability of such effects in future. © 2018 The Authors. Journal of Chemical Technology & Biotechnology published by JohnWiley & Sons Ltd on behalf of Society of Chemical Industry.
15 citations