Bioprocess

About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.

Nutrition and bioprocess development for efficient biosynthesis of an antitumor compound from marine-derived fungus.

Abstract: An integrated nutrition and bioprocess strategy was developed for improving the biosynthesis of an antitumor compound, 1403C, by a marine-derived fungus, Halorosellinia sp. (no. 1403). First, statistical design strategies were synthetically applied to optimize the nutritional composition. The resulting 1403C production reached 2.07 g/l, which was 143.5 % higher than the original production. However, it only produced 0.44 g/l of 1403C in 5-l bioreactor fermentation. Thus, the operating parameters including culture pH, dissolved oxygen, agitation speed, impeller type and inoculum level were considered to improve the fermentation process, and an effective control strategy for 1403C production by Halorosellinia sp. submerged in a 5-l bioreactor was established. When inoculating 0.22 g/l dry biomass, controlling dissolved oxygen not lower than 30 % during the growth phase but ranging between 30 and 40 % during the stationary phase, using a double-layer six-flat-blade Rushton disc turbine agitated at 400 rpm, keeping short-term low pH and rapid-rising pH with glucose starvation, the highest 1403C production was finally obtained at 1.32 g/l, which was promoted by 200 % compared to before optimization. Fermentation scale-up was finally performed in a 500-l bioreactor, and 1403C production of 1.09 g/l was obtained.

Biomass conversion technology : principles and practice

Abstract: This work on biotechnology examines the enormous potential of novel bioprocess techniques and processes to produce many new products from renewable resources, i.e., biomass. The authors cover conversion strategies (physical, chemical and biological) which are being developed to produce a wide range of products from biomass feedstocks. Improved methods of cultivating biomass materials via biotechnology are discussed and fundamental principles as well as practical applications are addressed. The book includes topics on pretreatment, alcohols, solvents, methane, fatty acids, food products and cellulases.

Development of bioprocess for high density cultivation yield of the probiotic Bacillus coagulans and its spores

Abstract: Bacillus coagulans is a spore forming lactic acid bacterium. Spore forming bacteria, have been extensively studied and commercialized as probiotics. Probiotics are produced by fermentation technology. There is a limitation to biomass produced by conventional modes of fermentation. With the great demand generated by range of probiotic products, biomass is becoming very valuable for several pharmaceutical, dairy and probiotic companies. Thus, there is a need to develop high cell density cultivation processes for enhanced biomass accumulation.The bioprocess development was carried out in 6.6l bench top lab scale fermentor. Four different cultivation strategies were employed to develop a bioprocess for higher growth and sporulation efficiencies of probiotic B. coagulans. Batch fermentation of B. coagulans yielded 18gl-1 biomass (as against 8.0gl-1 productivity in shake flask) with 60% spore efficiency. Fed batch fermentation was carried out for glucose, which yielded 25gl-1 of biomass. C/N ratio was very crucial in achieving higher spore titres. Maximum biomass yield recorded was 30gl-1, corresponding to 3.8x1011 cells ml-1 with 81% of cells in sporulated stage.The yield represents increment of 85 times the productivity and 158 times the spore titres relative to the highest reported values for high density cultivation of B. coagulans.

Developing Cellulolytic Organisms for Consolidated Bioprocessing of Lignocellulosics

Abstract: Lignocellulosic biomass represents an abundant, renewable feedstock for the production of biofuels and chemicals in a potentially sustainable manner. The main reason that it is not widely used at present is the technological barrier that there is no low-cost technology, either biological or thermochemical, to overcome the recalcitrance of lignocellulose. An organism that hydrolyzes the polysaccharides in biomass and simultaneously produces a commodity product such as ethanol at a high rate and titer could significantly reduce the costs of biomass conversion through the biological route. This would allow a combination of steps that are currently accomplished in different reactors into a consolidated bioprocess (CBP). While no ideal wild-type organism has been identified that can be used in CBP, several candidates are in various stages of development. This chapter assesses the status quo for CBP organismal development either by enabling noncellulolytic organisms to grow on cellulosic substrates, by improving product forming abilities of native cellulose utilizing organisms, or by engineering organisms with improved cellulolytic and product forming abilities. Furthermore, the state of the art of feedstock, pretreatment, and process integration options are briefly assessed.