Bioprocess

About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.

Dynamics in bioprocess development for Pichia pastoris.

Abstract: Pichia pastoris is a widely used host organism for the recombinant production of proteins. It is attracting increasing interest for the production of biopharmaceuticals, due to its capability of performing posttranslational modifications. Traditionally, production processes with P. pastoris describe fed-batch processes based on feed forward regimes with a constant specific growth rate. However, this strategy does not consider physiological changes of the organism, bearing the risk of overfeeding and thus harming the cells. Recently, we introduced the specific substrate uptake rate as a novel physiological parameter to design fed-batch strategies for P. pastoris. We showed that by doing dynamic batch experiments strain specific parameters, which are needed to set up respective feeding profiles, can be easily determined. Furthermore we proved that dynamics during feeding directly affects productivity and product purity. Here, we sum up our findings regarding dynamics in bioprocess development for P. pastoris.

Analysis of a diverse bacterial community and degradation of organic compounds in a bioprocess for coking wastewater treatment

Abstract: Coking wastewater is one of the most toxic industrial effluents since it contains high concentration of toxic organic compounds. Biological treatments are widely applied in coking wastewater treatment, pollutants can be degraded completely due to the synergistic effect of the community composition. In this study, the community structure and degradation of organic compounds of a full-scale coking wastewater treatment plant with anaerobic, anoxic, and oxic process (A1/A2/O) were studied. GC-MS results showed that phenols, indole, quinoline and pyridine, accounting for 61.70, 13.63, 7.71 and, 2.30%, respectively, were the main organic pollutants in the raw coking wastewater. Those pollutants were degraded gradually during the A1/A2/O bioprocess, respectively. High throughput pyrosequencing was applied to investigate the bacterial community, the sequences could be affiliated to 21 phylogenetic groups, including Proteobacteria, Chloroflexi, Bacteroidetes, Planctomycetes, Synergistetes, Chlorobi, Acidob...

Chemical, physical, and biochemical concepts in isolation and purification of proteins*

Abstract: * Reprinted from Separation Processes in Biotechnology; Asenjo, J.A., Ed.; Bioprocess Technology, Vol. 9; Marcel Dekker, Inc.; New York, 1990; 17–64.

Editorial: Bioprocess Development in the era of digitalization.

Abstract: In the last few decades, a vast number of research findings in biotechnological processes were reported with high commercial relevance. Still only a small fraction of these molecules was successfully brought to industrial production. The development of production processes in biotechnology still requires a lot of time and money compared to other industries. Due to the complexity of biological systems, the commercialization of cell-derived products needs a combination of deep scientific knowledge and a thorough understanding of process engineering. In addition, regulatory requirements must be met, which may increase the overall risk of success. Process development in biotechnology is still mainly driven by experienced personnel creating and evaluating experimental data. However, in recent times, automation, miniaturization and data science are setting new paths for drastic changes in bioprocess development. Today, hundreds of automated experiments can be performed in parallel miniaturized cultivation and purification systems per day. Additionally, statistical experimental planning and evaluation is applied to utilize the experimental capacity of these facilities efficiently. Still, to exploit the full potential of automated laboratories, innovative software concepts and workflows are needed. Moreover, the conditions in which small-scale experiments are performed need to resemble the production scale as closely as possible [1]. Today, there are numerous possibilities to create, collect, store, and share data. This has a significant potential in

Fermentative molecular biohydrogen production from cheese whey: present prospects and future strategy

Abstract: Waste-dependent fermentative routes for biohydrogen production present a possible scenario to produce hydrogen gas on a large scale in a sustainable way. Cheese whey contains a high portion of organic carbohydrate and other organic acids, which makes it a feasible substrate for biohydrogen production. In the present review, recent research progress related to fermentative technologies, which explore the potentiality of cheese whey for biohydrogen production as an effective tool on a large scale, has been analyzed systematically. In addition, application of multiple response surface methodology tools such as full factorial design, Box-Behnken model, and central composite design during fermentative biohydrogen production to study the interactive effects of different bioprocess variables for higher biohydrogen yield in batch, fed-batch, and continuous mode is also discussed. The current paper also emphasizes computational fluid dynamics-based simulation designs, by which the substrate conversion efficiency of the cheese whey-based bioprocess and temperature distribution toward the turbulent flow of reaction liquid can be enhanced. The possible future developments toward higher process efficiency are outlined.