Topic
Bioprocess
About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.
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TL;DR: Glucose concentration control of a bioreactor is not a trivial task due to high process variability, measurement noise and long measurement intervals but NMPC proved successful in achieving closed loop fixed set-point control in the presence of these common bioprocess operation attributes.
132 citations
TL;DR: In this review article, insights are provided into the different applications of infrared spectroscopy for bioprocess monitoring and the complex data interpretation.
Abstract: One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique to analyze a wide variety of organic compounds. Thus, infrared sensors are ideal instruments for bioprocess monitoring. The sensors are non-invasive, have no time delay due to sensor response times, and have no influence on the bioprocess itself. No sampling is necessary, and several components can be analyzed simultaneously. In general, the direct monitoring of substrates, products, metabolites, as well as the biomass itself is possible. In this review article, insights are provided into the different applications of infrared spectroscopy for bioprocess monitoring and the complex data interpretation. Different analytical techniques are presented as well as example applications in different areas.
130 citations
TL;DR: The rapid developments in biotechnology have resulted in the identification and use of a large variety of biologically active substances produced from microbial, plant and animal origin, which range from enzymes and antibiotics to highly complex molecules such as immunoglobulins, growth factors and hormones.
Abstract: The rapid developments in biotechnology have resulted in the identification and use of a large variety of biologically active substances produced from microbial, plant and animal origin. These range from enzymes and antibiotics to highly complex molecules such as immunoglobulins, growth factors and hormones. The advances in bioprocess technology have enabled the cultivation of different micro-organisms, namely bacteria, yeast and fungi, on a large scale. The potential use of plant and animal cells has, however, not yet been completely realized. One of the major limitations in the scale-up of plant and animal cell culture is the shear sensitivity of these cells. This arises owing to the large size of the cells. In addition, animal cells are especially fragile because of the lack of cell walls.
129 citations
TL;DR: This review discusses integrated processing for ethanol, butanol, organic acids, antibiotics, and other products, which has important advantages in improving yield and conversion relative to conventional processes.
128 citations
TL;DR: In conclusion, suspension culture protocols of hPSCs could be used to mass produce homogenous and pluripotent undifferentiated cells by identification and optimization of key bioprocess parameters that are complemented by a simple and rapid scale-up platform.
Abstract: Current protocols for the scalable suspension culture of human pluripotent stem cells (hPSCs) are limited by multiple biological and technical challenges that need to be addressed before their use in clinical trials. To overcome these challenges, we have developed a novel bioprocess platform for large-scale expansion of human embryonic and induced pluripotent stem cell lines as three-dimensional size-controlled aggregates. This novel bioprocess utilizes the stepwise optimization of both static and dynamic suspension culture conditions. After screening eight xeno-free media in static suspension culture and optimizing single-cell passaging in dynamic conditions, the scale-up from a static to a dynamic suspension culture in the stirred bioreactor resulted in a two- to threefold improvement in expansion rates, as measured by cell counts and metabolic activity. We successfully produced size-specific aggregates through optimization of bioreactor hydrodynamic conditions by using combinations of different agitati...
128 citations