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Bioprocess
About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.
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TL;DR: A fast and versatile analytical LC‐MS method has been set up, which has proven of much value for the in‐process analysis of macrotetrolides during fermentation and which has allowed rapid large‐scale bioprocess development.
Abstract: The optimization of the biosynthetic pathways is highly attractive for the large-scale preparation of macrotetrolides, because overall yields in the chemical synthesis of compounds like nonactin have been very low. A key success factor determining the outcome of such optimizations is the adequate process analysis for the envisioned product. The analytical methods for process control involved in the past spectrophotometric and chromatographic measurements. LC-MS offers a modern approach to obtain more detailed data than the spectrophotometric and chromatographic measurements used in the past. In this work, a fast and versatile analytical LC-MS method has been set up, which has proven of much value for the in-process analysis of macrotetrolides during fermentation and which has allowed rapid large-scale bioprocess development.
11 citations
TL;DR: In this paper, a multi-objective approach using desirability function generated through predictor importance and weighted coefficient methodology was adopted for optimization of Bioprocess nutritional conditions for production of glutaminase-near free L-asparaginase by Aspergillus candidus UCCM 00117 was conducted under shake flask laboratory conditions.
Abstract: Sequential optimization of bioprocess nutritional conditions for production of glutaminase-near-free L-asparaginase by Aspergillus candidus UCCM 00117 was conducted under shake flask laboratory conditions. Catalytic and anti-cancer activities of the poly-peptide were evaluated using standard in vitro biochemical methods. Medium nutrients were selected by one-factor-at-a-time (OFAT) approach while Plackett–Burman design (PBD) screened potential factors for optimization. Path of steepest ascent (PSA) and response surface methodology (RSM) of a Min-Run-Res V fractional factorial of a central composite rotatable design (CCRD) were employed to optimize factor levels towards improved enzyme activity. A multi-objective approach using desirability function generated through predictor importance and weighted coefficient methodology was adopted for optimization. The approach set optimum bioprocess conditions as 49.55 g/L molasses, 64.98% corn steep liquor, 44.23 g/L asparagine, 1.73 g/L potassium, 0.055 g/L manganese and 0.043 g/L chromium (III) ions, at a composite desirability of 0.943 and an L-asparaginase activity of 5216.95U. The Sephadex-200 partially-purified polypeptide had a specific activity of 476.84 U/mg; 0.087U glutaminase activity, 36.46% yield and 20-fold protein purification. Anti-cancer activity potentials of the catalytic poly-peptide were dose-dependent with IC50 (µg/mL): 4.063 (HL-60), 13.75 (HCT-116), 15.83 (HeLa), 11.68 (MCF-7), 7.61 (HepG-2). The therapeutic enzyme exhibited 15-fold more cytotoxicity to myeloid leukemia cell line than to normal (HEK 238 T) cell. Optimum temperature and pH for activity were within physiological range. However, significant interactions between exposure time and levels of each of temperature and pH made interpretations of residual enzyme activities difficult. The manganese-dependent L-asparaginase from Aspergillu s candidus UCCM 00117 is recommended for further anticancer drug investigations.
11 citations
TL;DR: Pullulan has unique physicochemical properties, due to which it has wide applications in the food, pharmaceutical and biomedical industries, and its applications are reviewed.
Abstract: Pullulan is an important exopolysaccharide commercially obtained from ubiquitous fungi Aureobasidium pullulans. Pullulan has unique physicochemical properties, due to which it has wide applications in the food, pharmaceutical and biomedical industries. Various synthetic media and agro-industrial wastes have been utilized for the production of pullulan. Agro-industrial waste has become a preferred substrate for biotransformation due to its economical aspect and nutritional attributes. Pullulan is one of the valuable products produced by the biotransformation of agro-based wastes. Numerous factors which influence the pullulan production are the type of substrate, the addition of carbon source, nitrogen source, pH of the medium, aeration/ agitation speed and temperature. The cost, productivity of the product and efficiency depends on the selection of raw material, type of fermentation (bioprocess), type of strain and downstream processing (recovery). This review focuses on pullulan production from agro-based wastes, the influence of factors and its applications.
11 citations