Topic
Bioprocess
About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.
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TL;DR: An overview of the advantages and disadvantages of the most commonly used technologies for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique.
106 citations
TL;DR: Perspectives technologiques de la lutte contre les insectes, aspects economiques, applications sur le terrain, and other aspects economiques.
Abstract: (1987). Bioprocess Developments in the Production of Bioinsecticides by Bacillus Thuringiensis. Critical Reviews in Biotechnology: Vol. 6, No. 1, pp. 87-127.
105 citations
TL;DR: By use of near-infrared (NIR) spectroscopy, simultaneous, multiple-constituent estimation of important bioprocess parameters can be obtained in a time frame (<1 min assay) that was previously unattainable.
Abstract: By use of near-infrared (NIR) spectroscopy, simultaneous, multiple-con-stituent estimation of important bioprocess parameters can be obtained in a time frame (< 1 min assay) that was previously unattainable. Therefore, with NIR spectroscopy the opportunity exists to incorporate real-time chemical information into bioprocess monitoring or control strategies which will lead to significant bioproeess improvements. The NIR spectroscopic analysis of unmodified whole broth samples for acetate, ammonium, biomass, and glycerol is described for an industrial Escherichia coil fed-batch fermentation bioprocess. For acetate and glycerol, suitable results were obtained from multiple linear least-squares regression (MLR) analysis. A more sophisticated partial least-squares (PLS) regression analysis was necessary to adequately model ammonium and biomass. The respective prediction errors (1σ) of 0.7 g/L, 1.4 g/L, 0.7 g/L, and 7 mmol/L for acetate, biomass, glycerol, and ammonium compare well with the error of the wet chemical reference methods used to derive the calibration algorithms.
105 citations
TL;DR: In this article, the authors proposed a method for the determination of metabolic variability in bioprocessing conditions and for the efficient design of metabolic engineering strategies in large-scale bioreactors.
102 citations
TL;DR: The advantages and limitations of MIR are discussed in the context of "rival" technologies, such as near infrared, focusing especially on employing such techniques in bioprocesses for real time measurements.
101 citations