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Bioprocess
About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.
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TL;DR: In this article, the authors reported significant improvements in the productivity and quality of therapeutic proteins produced in Chinese hamster ovary (CHO) cells since their establishment as host cells for biopharmaceutical production.
Abstract: Significant improvements in the productivity and quality of therapeutic proteins produced in Chinese hamster ovary (CHO) cells have been reported since their establishment as host cells for biopharmaceutical production. Initial advances in the field focused on engineering strategies to manipulate genes associated with proliferation, apoptosis, and various metabolic pathways. Process engineering efforts to optimize culture media, batch-feeding strategies and culture conditions, including temperature and osmolarity, were also reported. More recently, focus has shifted toward enhancing process consistency and product quality using systems biology quality by design-based approaches during process development. Integration of different data generated using omics technologies, such as genomics, transcriptomics, proteomics and metabolomics, has facilitated a greater understanding of CHO cell biology. These techniques have enabled the provision of global information on dynamic changes in cellular components associ...
57 citations
TL;DR: Novel and selective microbial amperometric biosensors that use Gluconobacter oxydans cells to monitor the bacterial bioconversion of glycerol to 1,3-propanediol (1, 3-PD) are described.
Abstract: Novel and selective microbial amperometric biosensors that use Gluconobacter oxydans cells to monitor the bacterial bioconversion of glycerol (Gly) to 1,3-propanediol (1,3-PD) are described. Two different mediators, ferricyanide and flexible polyvinylimidazole osmium functionalized polymer (Os-polymer), were employed to prepare two different microbial biosensors, both of which gave high detection performance. The good operational stabilities of both types of biosensor were underlined by the ability to detect 1,3-PD throughout 140 h of continuous operation. Both microbial biosensor systems showed excellent selectivity for 1,3-PD in the presence of a high excess of glycerol [selectivity ratios (1,3-PD/Gly) of 118 or 245 for the ferricyanide and Os-polymer systems, respectively]. Further, the robustness of each microbial biosensor was highlighted by the high reliability of 1,3-PD detection achieved (average RSD of standards <2%, and well below 4% for samples). The biosensor implementing the Os-polymer mediator exhibited high selectivity towards 1,3-PD detection and allowed moderate sample throughput (up to 12 h−1) when integrated into a flow system. This system was used to monitor the concentration of 1,3-PD during a real bioprocess. Results from biosensor assays of 1,3-PD in bioprocess samples taken throughout the fermentation were in a very good agreement with results obtained from reference HPLC assays (R2 = 0.999).
57 citations
TL;DR: The fundamental principle behind consolidated bioprocessing as a microbial phenomenon has been established through the successful expression of the major (hemi)cellulolytic and amylolytic activities in S. cerevisiae, and strains with higher levels of secreted enzymes and greater resistance to fermentation inhibitors are yielded.
Abstract: Conversion of cellulose, hemicellulose or starch to ethanol via a biological route requires enzymatic conversion of these substrates to monosaccharides that can be assimilated by a fermenting organism. Consolidation of these events in a single processing step via a cellulolytic or amylolytic microorganism(s) is a promising approach to low-cost production of fuels and chemicals. One strategy for developing a microorganism capable of such consolidated bioprocessing (CBP) involves engineering Saccharomyces cerevisiae to expresses a heterologous enzyme system enabling (hemi)cellulose or starch utilization. The fundamental principle behind consolidated bioprocessing as a microbial phenomenon has been established through the successful expression of the major (hemi)cellulolytic and amylolytic activities in S. cerevisiae. Various strains of S. cerevisiae were subsequently enabled to grow on cellobiose, amorphous and crystalline cellulose, xylan and various forms of starch through the combined expression of these activities. Furthermore, host cell engineering and adaptive evolution have yielded strains with higher levels of secreted enzymes and greater resistance to fermentation inhibitors. These breakthroughs bring the application of CBP at commercial scale ever closer. This mini-review discusses the current status of different aspects related to the engineering of S. cerevisiae for next generation ethanol production. © 2013 Society of Chemical Industry
57 citations
TL;DR: In this paper, the importance of integration of lignocellulosic biorefineries with existing petrochemical refineries, the technical challenges of industrialization, SWOT analysis, and future directions have been reviewed.
57 citations
TL;DR: In this paper , the importance of integration of lignocellulosic biorefineries with existing petrochemical refineries, the technical challenges of industrialization, SWOT analysis, and future directions have been reviewed.
57 citations