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Bioprocess
About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.
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TL;DR: In this article, an overview of the principles and applications of thermochemical processes (pyrolysis, torrefaction, hydrothermal liquefaction, and gasification) and biochemical processes involved in LC biomass biorefinery for potential biofuel applications is provided.
39 citations
TL;DR: The data show how the combination of metabolic and bioprocess engineering can be used for the production of selenized yeast enriched with beneficial Se-metabolites.
39 citations
TL;DR: The effects of the concentration of carbon sources, i.e., glucose, fructose, sucrose, citric acid, and glycerol, and nitrogen sources, and temperature on β‐lactamase production were investigated with B. licheniformis 749/C in laboratory scale bioreactors to demonstrate the bioprocess was at mass transfer limited condition.
Abstract: After screening potential β-lactamase producers in a medium containing penicillin G, an inducible ( Bacillus subtilis NRS 1125) and a constitutive ( Bacillus licheniformis 749/C ATCC 25972) β-lactamase producer were selected. As the highest enzyme activity was obtained with B. licheniformis 749/C, the effects of the concentration of carbon sources, i.e., glucose, fructose, sucrose, citric acid, and glycerol, and nitrogen sources, i.e., (NH4)2HPO4, NH4Cl, yeast extract, casamino acids and peptone, pH, and temperature on β-lactamase production were investigated with B. licheniformis 749/C in laboratory scale bioreactors. Among the investigated media, the highest volumetric activity was obtained as 270 U cm−3 in the medium containing 10.0 kg m−3 glucose, 1.18 kg m−3 (NH4)2HPO4, 8.0 kg m−3 yeast extract, and the salt solution at 32 °C and pH0 = 6.0. By using the designed medium, fermentation and oxygen transfer characteristics of the bioprocess were investigated at V = 3.0 dm3 bioreactor systems with a VR = 1.65 dm3 working volume at QO/ VR = 0.5 vvm and N = 500 min-1. At the beginning of the process the Damkohler number was <1, indicating that the process was at biochemical reaction limited condition; at t = 2–5 h both mass-transfer and biochemical reaction resistances were effective; and at t = 6–10 h ( Da ≫1) the bioprocess was at mass transfer limited condition. Overall oxygen transfer coefficients ( KLa) varied between 0.01 and 0.03 s−1, enhancement factor ( KLa/ KLaO) varied between 1.2 and 2.3, and volumetric oxygen uptake rate varied between 0.001 and 0.003 mol m−3 s−1 throughout the bioprocess. The specific oxygen uptake and the specific substrate consumption rates were the highest at t = 2 h and then decreased with the cultivation. The maximum yield of cells on substrate and the maximum yield of cells on oxygen values were obtained, respectively, as YX/S = 0.34 and YX/O = 1.40, at t = 5 h, whereas the highest yield of substrate on oxygen was obtained as YS/O = 6.94 at t = 3.5 h. The rate of oxygen consumption for maintenance and the rate of substrate consumption for maintenance values were found, respectively, as mO = 0.13 kg kg−1 h−1 and mS = 3.02 kg kg−1 h−1.
39 citations
TL;DR: This approach increases the information content of cultivations in 96-microwell plates, thus turning them into a suitable tool for high-throughput bioprocess development and enables automated process monitoring at dedicated intervals.
Abstract: The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single steps. We aim to face this challenge by an interdisciplinary approach to significantly shorten development times and costs. As a first step, we scaled down analytical assays to the microliter scale and created automated procedures for starting the cultivation and monitoring the optical density (OD), pH, concentrations of glucose and acetate in the culture medium, and product formation in fed-batch cultures in the 96-well format. Then, the separate measurements of pH, OD, and concentrations of acetate and glucose were combined to one method. This method enables automated process monitoring at dedicated intervals (e.g., also during the night). By this approach, we managed to increase the information content of cultivations in 96-microwell plates, thus turning them into a suitable tool for high-throughput bioprocess development. Here, we present the flowcharts as well as cultivation data of our automation approach.
39 citations
TL;DR: Process engineers and scientists use simulation models to investigate complex and integrated biochemical operations, without the need for extensive experimentation.
Abstract: Maximizing profits by operating the most efficient process is the primary goal of all industrial bioprocessing operations. To help create efficient operations companies use process simulation, which is the application of a range of software tools to analyze complete processes, not just single unit operations. Process engineers and scientists use simulation models to investigate complex and integrated biochemical operations, without the need for extensive experimentation.
39 citations