Bioprocess

About: Bioprocess is a research topic. Over the lifetime, 2219 publications have been published within this topic receiving 50972 citations.

Removal of agent from cell suspension

Abstract: The invention relates to a method of removing an agent from a suspension of cells using a semi-permeable membrane. In one aspect of the invention, the cells are used to bioprocess a biological fluid after removal of the agent.

High-throughput screening and process optimisation

Abstract: Introduction The biological production of active compounds, ranging from small molecules, such as organic acids, vitamins or antibiotics, through to macromolecules, such as therapeutic proteins or plasmid gene therapy vectors, is of great commercial and social value. The cornerstone of any such bioprocess is the cell cultivation step where a highly selected, and usually engineered, cell-line is grown under carefully controlled conditions. The term cell-line is used here to represent both microbial and mammalian cells. The aim of the cultivation step is to yield the product in as an efficient and cost-effective manner as possible. However, the design and implementation of a cell cultivation process is often a complex, lengthy and costly task. The development of a cell cultivation process typically involves four stages, as shown in Fig. 12.1. Stage 1 involves the initial identification of a native, or wild-type, cell-line that produces the compound of interest, though usually slowly and at low levels. This is followed by Stage 2 in which the productivity of the chosen cell-line [g product (g cells) −1 h −1 ] is enhanced using a variety of microbiological and molecular biology techniques. Stage 3 involves optimisation of the growth media composition and culture conditions; while in Stage 4 , scale-up of the process from laboratory, through pilot plant to manufacturing scale occurs. High-throughput experimentation Traditionally cell culture development has been carried out following a sequential series of experiments, using conventional apparatus with a high labour requirement. Conventional experimentation usually involves carrying out one or only a few experiments at a time.

Hybrid Approach to State Estimation for Bioprocess Control.

Abstract: An improved state estimation technique for bioprocess control applications is proposed where a hybrid version of the Unscented Kalman Filter (UKF) is employed. The underlying dynamic system model is formulated as a conventional system of ordinary differential equations based on the mass balances of the state variables biomass, substrate, and product, while the observation model, describing the less established relationship between the state variables and the measurement quantities, is formulated in a data driven way. The latter is formulated by means of a support vector regression (SVR) model. The UKF is applied to a recombinant therapeutic protein production process using Escherichia coli bacteria. Additionally, the state vector was extended by the specific biomass growth rate µ in order to allow for the estimation of this key variable which is crucial for the implementation of innovative control algorithms in recombinant therapeutic protein production processes. The state estimates depict a sufficiently low noise level which goes perfectly with different advanced bioprocess control applications.

Apoptosis and Bioprocess Technology

Abstract: Optimisation of the production of biopharmaceuticals in animal cell lines has become a key area of research. The identification of apoptosis as the major mechanism of cell death during such processes has raised the importance of studies of cell death when implementing culture optimisation strategies. In this article we present an overview of the studies which have demonstrated the induction of apoptosis during the cultivation of industrially important animal cell lines. We also discuss studies which have shown that deprivation of factors such as amino acids, glucose, serum and oxygen are potent inducers of apoptosis in industrial cultures. The suppression of apoptosis under these conditions has been demonstrated by a number of recent reports, and we describe ways in which this knowledge may be applied in the development of novel solutions to some of the technical problems associated with the development of successful large scale culture process. The article concludes with a discussion of future directions for apoptosis research in bioprocess technology.