Topic
Bioreactor
About: Bioreactor is a research topic. Over the lifetime, 9980 publications have been published within this topic receiving 192690 citations. The topic is also known as: bioreactors.
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TL;DR: Statistical medium optimization improved volumetric pectinase productivity by about 2.8 folds and feeding sucrose as a feeding substrate with a rate of 2 g/L/h increased the enzyme production up to 450 U/mL after 126 h.
Abstract: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020. The production medium was first optimized using a statistical optimization approach to increase pectinase production. A maximal enzyme concentration of 76.35 U/mL (a 2.8-fold increase compared with the initial medium) was produced in a medium composed of (g/L): pectin, 32.22; (NH4)2SO4, 4.33; K2HPO4, 1.36; MgSO4.5H2O, 0.05; KCl, 0.05; and FeSO4.5H2O, 0.10. The cultivations were then carried out in a 16-L stirred tank bioreactor in both batch and fed-batch modes to improve enzyme production, which is an important step for bioprocess industrialization. Controlling the pH at 5.5 during cultivation yielded a pectinase production of 109.63 U/mL, which was about 10% higher than the uncontrolled pH culture. Furthermore, fed-batch cultivation using sucrose as a feeding substrate with a rate of 2 g/L/h increased the enzyme production up to 450 U/mL after 126 h. Statistical medium optimization improved volumetric pectinase productivity by about 2.8 folds. Scaling-up the production process in 16-L semi-industrial stirred tank bioreactor under controlled pH further enhanced pectinase production by about 4-folds. Finally, bioreactor fed-batch cultivation using constant carbon source feeding increased maximal volumetric enzyme production by about 16.5-folds from the initial starting conditions.
56 citations
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TL;DR: In this paper, a microaerobic membrane bioreactor seeded with anaerobic granular sludge was investigated for simultaneous removal of organic substances and nitrogen from domestic synthetic wastewaters.
56 citations
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TL;DR: The results confirmed the viability of rapid aerobic bio-stabilization in an aerated landfill bioreactor operated at various ORP levels (−400 to 150 mV) and increased the possibility of nitrification by promoting nitrifying bacteria having high oxygen affinities.
56 citations
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TL;DR: Investigation of the continuous production of 1,3-PDO with immobilized cells using raw glycerol without purification obtained from a biodiesel production process revealed that HRT is an important factor for both immobilized and suspended systems and a HRT of 2 h is the best one in terms of volumetric production rate.
Abstract: The continuous production of 1,3-propanediol (1,3-PDO) was investigated with Clostridium beijerinckii NRRL B-593 using raw glycerol without purification obtained from a biodiesel production process. Ceramic rings and pumice stones were used for cell immobilization in a packed-bed bioreactor. For comparison purpose, a control bioreactor with suspended culture was also run. The effect of hydraulic retention time (HRT) on the production of 1,3-PDO in both immobilized and suspended bioreactors were also investigated. The study revealed that HRT is an important factor for both immobilized and suspended systems and a HRT of 2 h is the best one in terms of volumetric production rate (g 1,3-PDO/L/h). Furthermore, cell immobilization had also obvious benefits especially for the robustness and the reliability of the production. The results indicated that cell immobilization achieved a 2.5-fold higher productivity in comparison to suspended cell system. Based on our results, continuous production of 1,3-PDO with immobilized cells is an efficient method, and raw glycerol can be utilized without any pretreatment.
56 citations
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TL;DR: Analysis of sludge MBR technology with algae to establish an effective wastewater treatment and low membrane fouling system revealed that the symbiosis of algae and sludge displayed more remarkable impacts on nutrients removal than either of them.
56 citations