Topic
Bioreactor
About: Bioreactor is a research topic. Over the lifetime, 9980 publications have been published within this topic receiving 192690 citations. The topic is also known as: bioreactors.
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TL;DR: The performance of a laboratory-scale anaerobic bioreactor was investigated to determine methane (CH4) content in biogas yield from digestion of organic fraction of municipal solid waste (OFMSW), aimed to focus on the effects of various factors, such as pH, moisture content (MC), total volatile solids (TVS), volatile fatty acids (VFAs), and CH4 fraction onBiogas production.
94 citations
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TL;DR: The model predicted the NH 4 +, COD, NO 3 - and TN profiles along the height of the bioreactor reasonably well and is a useful tool in design and analysis of SND in a fluidized bed biofilm reactor.
94 citations
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TL;DR: In this article, an up-flow packed bed bioreactor (UFPBBR) with immobilized Rhodococcus opacus onto biochar loaded polyurethane foam (PUF) as the packing material for treating biomass gasification wastewater (BGWW).
94 citations
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TL;DR: The photobioreactor is a cost‐efficient process which reduces the cost of aeration and prevents volatilization problems associated with the degradation of toxic volatile organic compounds under aerobic conditions.
Abstract: A consortium consisting of a Chlorella sorokiniana strain and a Ralstonia basilensis strain was able to carry out sodium salicylate biodegradation in a continuous stirred tank reactor (CSTR) using exclusively photosynthetic oxygenation. Salicylate biodegradation depended on algal activity, which itself was a function of microalgal concentration, light intensity, and temperature. Biomass recirculation improved the photobioreactor performance by up to 44% but the results showed the existence of an optimal biomass concentration above which dark respiration started to occur and the process efficiency started to decline. The salicylate removal efficiency increased by a factor of 3 when illumination was increased from 50 - 300 muE/M-2 (.)s. In addition, the removal rate of sodium salicylate was shown to be temperature-dependent, increasing from 14 to 27 mg/l(.)h when the temperature was raised from 26.5 to 31.5degreesC. Under optimized conditions (300 muE/m(2) (.)s, 30degreesC, 1 g sodium salicylate/l in the feed and biomass recirculation) sodium salicylate was removed at a maximum constant rate of 87 mg/l.h, corresponding to an estimated oxygenation capacity of 77 mg O-2/l(.)h (based on a BOD value of 0.88 g O-2/g sodium salicylate for the tested bacterium), which is in the range of the oxygen transfer capacity of large-scale mechanical surface aerators. Thus, although higher degradation rates were attained in the control reactor, the photobioreactor is a cost-efficient process which reduces the cost of aeration and prevents volatilization problems associated with the degradation of toxic volatile organic compounds under aerobic conditions. (C) 2004 Wiley Periodicals, Inc. (Less)
94 citations
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TL;DR: Aim: Statistical medium optimization for maximum production of a hyperthermostable lipase from Burkholderia cepacia and its validation in a bioreactor.
Abstract: Aim: Statistical medium optimization for maximum production of a hyperthermostable lipase from Burkholderia cepacia and its validation in a bioreactor.
Methods and Results:Burkholderia cepacia was grown in shake flasks containing 1% glucose, 0·1% KH2PO4, 0·5% NH4Cl, 0·24% (NH4)2HPO4, 0·01% MgSO4.7H2O and 1% emulsified palm oil, at 45 °C and pH 7·0, agitated at 250 rev min−1 with 6-h-old inoculum (2% v/v) for 20 h. A fourfold enhancement in lipase production (50 U ml−1) and an approximately three fold increase in specific activity (160 U mg−1) by B. cepacia was obtained in a 14 litre bioreactor within 15 h after statistical optimization following shake flask culture. The statistical model was obtained using face centred central composite design (FCCCD) with five variables: glucose, palm oil, incubation time, inoculum density and agitation. The model suggested no interactive effect of the five factors, although incubation period, inoculum and carbon concentration were the important variables.
Conclusions: The maximum lipase production was 50 U ml−1, with specific activity 160 U mg−1 protein, in a 14 litre bioreactor after 15 h in a medium obtained after statistical optimization in shake flasks. Further, the model predicted reduction in time for lipase production with reduction in total carbon supply.
Significance and Impact of the Study: Statistical optimization allows quick optimization of a large number of variables. It also provides a deep insight into the regulatory role of various parameters involved in enzyme production.
94 citations