scispace - formally typeset
Search or ask a question
Topic

Bovine serum albumin

About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.


Papers
More filters
Journal ArticleDOI
TL;DR: A remarkable decrease in the in vitro DL cell proliferation and induction of the apoptosis by 1-4 is revealed, which lies in the order 2 > 1 > 4 > 3.
Abstract: Syntheses and characterizations of the arene ruthenium [(η6-C6H6)RuCl(4-mtdpm)] (1), [(η6-p-MeC6H4Pri)RuCl(4-mtdpm)] (2), and structurally analogous rhodium/iridium complexes [(η5-C5Me5)RhCl(4-mtdpm)] (3) and [(η5-C5Me5)IrCl(4-mtdpm)] (4) [4-mtdpm = 5-(4-methylthiophenyl)dipyrromethene] have been reported. Their identities have been established by satisfactory elemental analyses, electrospray ionization-mass spectrometry (ESI-MS), FT-IR, NMR (1H, 13C), UV/vis, emission spectral, and electrochemical studies. Structure of the representative complex 3 has been authenticated by X-ray single crystal analyses. The complexes 1–4 effectively bind with calf thymus DNA (CT DNA) through intercalative/electrostatic interactions. In addition, these exhibit significant cytotoxicity toward Dalton lymphoma (DL) cell line and cause static quenching of the bovine serum albumin (BSA) fluorophore. The antiproliferative activity, morphological changes, and apoptosis have been evaluated by MTT assay, acridine orange/ethidium b...

140 citations

Journal ArticleDOI
12 Jul 2005-Langmuir
TL;DR: The results imply that the use of charged conjugated polymers as biosensors, while an attractive proposition, has to take into account strong nonspecific interactions between conjugate polymers and the host of proteins that is found in cells and complex biological fluids.
Abstract: Two carboxylate-substituted, fluorescent (Φ = 0.08), water-soluble poly(p-phenyleneethynylene)s (PPE) and a water-soluble model compound were exposed to a series of proteins and bovine serum. While the anionic PPEs do not have any specific binding sites, they form stable complexes with histone, lysozyme, myoglobin, and hemoglobin. The complex formation was evidenced by fluorescence quenching. Bovine serum albumin does not quench the fluorescence of the PPEs but enhances it, probably due to its surfactant character. These results imply that the use of charged conjugated polymers as biosensors, while an attractive proposition, has to take into account strong nonspecific interactions between conjugated polymers and the host of proteins that is found in cells and complex biological fluids.

139 citations

Journal ArticleDOI
TL;DR: The biosensor was evaluated for detection of protein biomarkers relevant to cancer diagnostics--human chorionic gonadotropin (hCG) and activated leukocyte cell adhesion molecule (ALCAM) both in buffer and in 10% blood plasma.

139 citations

Journal ArticleDOI
TL;DR: In this paper, bovine serum albumin (BSA) was probed by tensiometry, ellipsometry, and infrared reflection-absorption spectroscopy (IRRAS).

139 citations

Journal ArticleDOI
TL;DR: Fluorescence data and UV-vis and CD results showed that the addition of CdTe QDs changed the conformation of BSA and hydrogen bonds and van der Waals forces between the protein and the QDs are the main binding forces stabilizing the complex.

139 citations


Network Information
Related Topics (5)
Amino acid
124.9K papers, 4M citations
87% related
Cell culture
133.3K papers, 5.3M citations
84% related
Aqueous solution
189.5K papers, 3.4M citations
83% related
DNA
107.1K papers, 4.7M citations
83% related
Peptide sequence
84.1K papers, 4.3M citations
82% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023475
2022983
2021423
2020460
2019468
2018489