Topic
Bovine serum albumin
About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.
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TL;DR: Results indicate the potential of controlled release approach in cytokine-based treatment protocols of tumors, and show significant longer survival rates of mice treated with IL-1α carrying microspheres, on days 7 and 12 after tumor cell inoculation, while mice treatment with empty microsphere died at similar rates to those observed in non-treated tumor-bearing mice.
123 citations
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TL;DR: Viscoelastic behavior indicated a tenuous network, solidlike at low strain but re-forming after breakage by shear, and high MW sensitivity was observed by rheology for the terminal time, which increased as well with the strength of polyelectrolyte-protein interaction.
123 citations
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TL;DR: It was proved that the fluorescence quenching of BSA by flavonoids was a result of the formation of a flavonoid–BSA complex, and the results of thermodynamic parameters ΔG, ΔH, and ΔS at different temperatures indicated that the hydrophobic interaction played a major role in Flavonoid-BSA association.
Abstract: Flavonoids are structurally diverse and the most ubiquitous groups of dietary polyphenols distributed in various fruits and vegetables. In this study, the interaction between five flavonoids, namely formononetin-7-O-β-D-glucoside, calycosin- 7-O-β-D-glucoside, calycosin, rutin, and quercetin, and bovine serum albumin (BSA) was investigated by fluorescence and UV-vis absorbance spectroscopy. In the discussion, it was proved that the fluorescence quenching of BSA by flavonoids was a result of the formation of a flavonoid-BSA complex. Fluorescence quenching constants were determined using the Stern-Volmer and Lineweaver-Burk equations to provide a measure of the binding affinity between the flavonoids and BSA. The binding constants ranked in the order quercetin>rutin>calycosin>calycosin-7-O-β-D-glucoside ≈ formononetin-7-O-β-D-glucoside. The results of thermodynamic parameters ΔG, ΔH, and ΔS at different temperatures indicated that the hydrophobic interaction played a major role in flavonoid-BSA association. The distance r between BSA and acceptor flavonoids was also obtained according to Forster's theory of non-radiative energy transfer.
123 citations
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TL;DR: The idea that serum deprivation and/or removal of the cumulus cells during oocyte maturation in vitro results in an alteration of the zona pellucida that is manifested as an increased resistance to proteolytic digestion and sperm penetration is supported.
Abstract: The effects of serum and cumulus cells during oocyte maturation in vitro on subsequent oocyte fertilizability and zona pellucida digestability have been examined. Cumulus cell-enclosed oocytes were cultured 15–16 hours in medium containing bovine serum albumin plus varying concentrations of fetal bovine serum (FBS). When the serum concentration was decreased incrementally from 5% to 0%, resistance to chymotrypsin digestion increased accordingly in a dose-dependent manner. The zona pellucida digestion time for oocytes matured in serum-free medium was increased nearly 500% above that for control oocytes matured in 5% FBS. Also, fertilization was decreased as serum concentration was lowered; the fertilization percentage for oocytes matured in serum-free serum was reduced by over 90%. This loss of fertilizability was correlated with an absence of sperm within the vitellus following insemination of matured ova. Increasing the serum concentration from 5% to 10% had no effect on fertilization or zona pellucida digestion time. Serum deprivation, even for a short period of time, at the onset of culture significantly reduced the fertilizability of cumulus cell-enclosed oocytes and increased the zona pellucida digestion time. Removal of serum after oocyte maturation in vitro had little effect. The presence of an intact cumulus oophorus during maturation in vitro was important in the maintenance of fertilizability and zona digestability. These data support the idea that serum deprivation and/or removal of the cumulus cells during oocyte maturation in vitro results in an alteration of the zona pellucida that is manifested as an increased resistance to proteolytic digestion and sperm penetration.
123 citations
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TL;DR: Structural analysis showed that both Sudan II and Sudan IV interact mainly with BSA at the hydrophobic pocket and via Van der Waals forces, suggesting a partial protein unfolding.
123 citations