Topic
Bovine serum albumin
About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.
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01 Jan 1966
TL;DR: The results are discussed in terms of the gel structure and a method for estimating the average effective pore radius in polyacrylamide gels of different compositions is derived and compared with experimental findings.
Abstract: A method for molecular-sieve chromatography of proteins on columns of granulated polyacrylamide gels is described in detail. Distribution coefficients (K D and K av) are given for the model proteins γ globulin, bovine serum albumin, ovalbumin, ovomucoid, pepsin, myoglobin, and cytochrome c, as well as certain other proteins, between 0.5 M NaCl and polyacrylamide gels of 30 different compositions. The results are interpreted in terms of the Ogston-Laurent-Killander theory of molecular-sieve chromatography, and empirical equations are derived relating the fundamental parameters L and r of this theory to the gel composition. The results are discussed in terms of the gel structure and a method for estimating the average effective pore radius in polyacrylamide gels of different compositions is derived and compared with experimental findings.
118 citations
TL;DR: It is demonstrated that RuII(CO)2–protein complexes, formed by the reaction of the hydrolytic decomposition products of [fac-RuCl(κ2-H2NCH2CO2)(CO)3) with histidine residues exposed on the surface of proteins, spontaneously release CO in aqueous solution, cells, and mice.
Abstract: We demonstrate that RuII(CO)2–protein complexes, formed by the reaction of the hydrolytic decomposition products of [fac-RuCl(κ2-H2NCH2CO2)(CO)3] (CORM-3) with histidine residues exposed on the surface of proteins, spontaneously release CO in aqueous solution, cells, and mice. CO release was detected by mass spectrometry (MS) and confocal microscopy using a CO-responsive turn-on fluorescent probe. These findings support our hypothesis that plasma proteins act as CO carriers after in vivo administration of CORM-3. CO released from a synthetic bovine serum albumin (BSA)–RuII(CO)2 complex leads to downregulation of the cytokines interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α in cancer cells. Finally, administration of BSA–RuII(CO)2 in mice bearing a colon carcinoma tumor results in enhanced CO accumulation at the tumor. Our data suggest the use of RuII(CO)2–protein complexes as viable alternatives for the safe and spatially controlled delivery of therapeutic CO in vivo.
118 citations
TL;DR: The results show that glycation process affects the native structure of BSA, and the partial unfolding of the tertiary structure which accompanies the aggregation process is similar both in native and glycated BSA.
118 citations
TL;DR: It appears that the soluble form of guanylate cyclase from rat lung exists as a dimer, and specific activities in excess of 1 mumol of cyclic GMP formed/min/mg of enzyme protein can be obtained.
118 citations
TL;DR: The effects of albumin on the geometry of human erythrocytes have been studied as discussed by the authors, where individual red cells, hanging on edge from coverslips, were photographed and digitized using a Gradicon digitizer.
118 citations