Topic
Bovine serum albumin
About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.
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TL;DR: It was proved that the probable quenching mechanism of BSA by quinclorac was dynamic quenched and the alterations of protein secondary structure in the presence of QUC were confirmed by the evidences from three-dimensional fluorescence, synchronous fluorescence and CD spectroscopy.
116 citations
15 Apr 1997
TL;DR: In this article, the adsorption of bovine serum albumin (BSA) at the TiO 2aqueous NaCl interface was studied as a function of pH and electrolyte concentration using kinetic and steady-state measurements.
Abstract: The adsorption of bovine serum albumin (BSA) at the TiO 2 –aqueous NaCl interface was studied as a function of pH and electrolyte concentration using kinetic and steady-state measurements. The electrokinetic properties of BSA-covered TiO 2 particles and the acid–base character of the protein were monitored by electrophoretic mobilities and acid–base potentiometric titration, respectively. Some experiments were also outlined to analyze the different affinities of BSA monomers, dimers, and polymers for the surface. The adsorption process is fast; less than 10 min is enough to achieve steady-state conditions. All measured isotherms showed high initial slopes even under adverse electrostatic conditions, resulting in high-affinity isotherms. Adsorption plateau values (Γ max ) had a great pH dependence; Γ max –pH curves reached a maximum at around the isoelectric point of BSA and showed a drastic decrease on the acid side. Both structural and electrostatic effects must be invoked to explain the diminution of adsorbed BSA on either side of the isoelectric point. Structural effects were related to the different conformational states that BSA molecules adopt with pH changes, whereas electrostatic effects were analyzed assuming that BSA molecules behave as soft particles. This reasoning also allows us to explain the independence of the Γ max –pH curves from electrolyte concentration.
116 citations
TL;DR: The receptor specific interactions of GNR-BBN conjugates provide realistic opportunities in the design and development of in vivo molecular imaging and therapy agents for cancer.
Abstract: Gastrin releasing protein receptor specific bombesin (BBN) peptide-gold nanoconjugates were successfully synthesized using gold nanorods and dithiolated peptide. The gold nanorod-bombesin (GNR-BBN) conjugates showed extraordinary in vitro stabilities against various biomolecules including NaCl, cysteine, histidine, bovine serum albumin, human serum albumin, and dithiothreitol. Quantitative measurements on the binding affinity (IC(50)) of GNR-BBN conjugates toward prostate and breast tumor cells were evaluated. The IC(50) values establish that GNR-BBN conjugates have strong affinity toward the gastrin releasing peptide receptors on both the tumors. Detailed cellular interaction studies of GNR-BBN conjugates revealed that nanorods internalize via a receptor-mediated endocytosis pathway. The receptor specific interactions of GNR-BBN conjugates provide realistic opportunities in the design and development of in vivo molecular imaging and therapy agents for cancer.
116 citations
TL;DR: The result of synchronous fluorescence spectra shows that the conformation of bovine serum albumin has been changed at the present of 6-mercaptopurine, which indicates dynamic quenching mechanism.
Abstract: Fluorescence quenching in solutions of bovine serum albumin has been investigated in the presence of 6-mercaptopurine and ionic surfactants. Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of bovine serum albumin by 6-mercaptopurine was dynamic quenching mechanism. The Stern–Volmer quenching model has been successfully applied, and the activation energy of the interaction between 6-mercaptopurine and bovine serum albumin as much as 4.26 kJ mol−1 was calculated. The distance r between donor (bovine serum albumin) and acceptor (6-mercaptopurine) was obtained according to fluorescence resonance energy transfer (FRET). The result of synchronous fluorescence spectra shows that the conformation of bovine serum albumin has been changed at the present of 6-mercaptopurine.
116 citations
TL;DR: In situ perfusion showed that brain uptake was improved by up to 98% for several of the glycosylated peptides, and the nociceptive profiles of the peptides followed the rank order of peptide entry to the brain with up to a 39-fold increase in A.C.U.
116 citations