Topic
Bovine serum albumin
About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.
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TL;DR: The effect of temperature between 4 and 60° supports the interpretation that the spectral shifts reflect nanosecond relaxations of polar groups around the dye during the lifetime of the excited state.
109 citations
TL;DR: It is found for the first time that the complexation could take place well above the critical pH(c), the value that most previous studies had regarded as the onset occurrence of complexation.
Abstract: The complexation between bovine serum albumin (BSA) and sugar beet pectin (SBP) was studied in situ by coupling glucono-δ-lactone (GDL) induced acidification with dynamic light scattering and turbidity measurements. Individual measurements at specific pHs and mixing ratios were also carried out using zeta potentiometry, gel permeation chromatography-multiangle laser light scattering (GPC-MALLS), and isothermal titration calorimetry (ITC). These investigations together enabled the establishment of a phase diagram of BSA/SBP and the identification of the molecular events during protein/polysaccharide complexation in relation to the phase diagram, which showed five regions: (I) a stable region of mixed individual soluble polymers, (II) a stable region of intramolecular soluble complexes, (III) a quasi-stable region of intermolecular soluble complexes, (IV) an unstable region of intermolecular insoluble complexes, and (V) a second stable region of mixed individual soluble polymers, on lowering pH. We found for the first time that the complexation could take place well above the critical pH(c), the value that most previous studies had regarded as the onset occurrence of complexation. A model of structural transitions between the regions was proposed. The borderline between region II and region III represents the BSA/SBP stoichiometry for intramolecular soluble complex at a specific pH, while that between region III and region IV identifies the composition of the intermolecular insoluble complex. Also studied was the effect of NaCl and CaCl(2) on the phase diagram and structural transitions.
109 citations
TL;DR: At Atomic force microscopy and surface plasmon resonance (SPR) analysis revealed that the surface coverage of the dextran monolayers increased with an increasing degree of thiol substitution, but conversely decreased with increasing molecular weight.
109 citations
TL;DR: It was decided to reisolate this modified peptide and to determine its amino acid sequence and to establish the identity of an amino acid residue in one of the binding sites of the albumin molecule.
109 citations
TL;DR: A novel Förster donor-acceptor dye pair for an immunoassay based on resonance energy transfer (RET) is characterized with respect to its photophysical properties and the applicability of this dye pair is demonstrated in a homogeneous competitive immunoASSay for the pesticide simazine.
109 citations