Topic
Bovine serum albumin
About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.
Papers published on a yearly basis
Papers
More filters
TL;DR: Chromatography on GlycoGel B boronic acid affinity gel indicates that 10-12% of normal serum albumin is glycosylated, which is approximately 9 times that of human hemoglobin.
317 citations
TL;DR: The complexes of a carboxylate-substituted poly(para-phenyleneethynylene) (PPE) with histone, bovine serum albumin, and papain were investigated, and the observed selectivities and sensitivities are most unusual.
Abstract: The complexes of a carboxylate-substituted poly(para-phenyleneethynylene) (PPE) with histone, bovine serum albumin, and papain were investigated. At higher concentrations, the proteins have a significant effect on the emission characteristics of the PPE. The electrostatic complexes formed from the PPE, and the proteins are agglutinated by different metal cations. The observed selectivities and sensitivities of these simple systems are most unusual; combination of the carboxylate-PPE and commercially available papain forms an electrostatic complex that is precipitated by mercury ions at concentrations above 10-5 mol L-1.
316 citations
TL;DR: The use of bovine serum albumin is recommended as a primary standard for serum protein assays because it is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.
Abstract: We have studied the standardization of total serum protein assay with the biuret reaction. Standard solutions were prepared from lyophilized preparations of human serum albumin and bovine serum albumin, with corrections made for volatile material and ash contents. These solutions and a solution of crystalline albumin standard were analyzed with a new stable biuret reagent, to establish absorptivity values (values for the absorbance of a 1 g/liter final reaction mixture). The mean values obtained were 0.302, 0.292, and 0.290 for human serum albumin, bovine serum albumin, and the crystalline albumin, respectively. We believe that the established absorptivity value will improve the accuracy of serum protein determinations. We studied the linearity of the relation between color produced and protein concentration, with use of the solutions described above and a serum pool. The color adheres to Beer's law up to the highest concentration tested: 3 g/liter for HSA and BSA, and 2.8 g/liter for serum in the final reaction mixture. The new biuret reagent has been stable for one year at room temperature. We recommend the use of bovine serum albumin as a primary standard for serum protein assays. It is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.
314 citations
TL;DR: The authors measured ionic current blockages caused by protein translocation through voltage-biased silicon nitride nanopores in ionic solution and demonstrated that a larger protein fibrinogen could be distinguished from BSA by a solid-state nanopore measurement.
Abstract: The authors measured ionic current blockages caused by protein translocation through voltage-biased silicon nitride nanopores in ionic solution. By calculating the mean amplitude, time duration, and the integral of current blockages, they estimated the relative charge and size of protein molecules at a single molecule level. The authors measured the change in protein charge of bovine serum albumin (BSA) protein induced by pH variation. They also confirmed that BSA molecules indeed traverse nanopores using an improved chemiluminescent analysis. They demonstrated that a larger protein fibrinogen could be distinguished from BSA by a solid-state nanopore measurement.
311 citations
TL;DR: Cationized albumin provides a new tool for studying absorptive-mediated endocytosis at the brain capillary and may also provide a vehicle for directed drug delivery through the blood-brain barrier.
310 citations