Topic
Bovine serum albumin
About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.
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TL;DR: The studied results by FT-IR and CD experiment indicated that the secondary structures of protein have been perturbed by the interaction of wogonin with BSA.
246 citations
TL;DR: The disparate effects of a givenprotein corona on particle-cell interactions are demonstrated, highlighting the correlation between protein corona conformation in situ and relevant biological characteristics for biological functionalities.
Abstract: Many biomolecules, mainly proteins, adsorb onto polymer particles to form a dynamic protein corona in biological environments. The protein corona can significantly influence particle-cell interactions, including internalization and pathway activation. In this work, we demonstrate the differential roles of a given protein corona formed in cell culture media in particle uptake by monocytes and macrophages. By exposing disulfide-stabilized poly(methacrylic acid) nanoporous polymer particles (PMASH NPPs) to complete cell growth media containing 10% fetal bovine serum, a protein corona, with the most abundant component being bovine serum albumin, was characterized. Upon adsorption onto the PMASH NPPs, native bovine serum albumin (BSA) was found to undergo conformational changes. The denatured BSA led to a significant decrease in internalization efficiency in human monocytic cells, THP-1, compared with the bare particles, due to reduced cell membrane adhesion. In contrast, the unfolded BSA on the NPPs triggered class A scavenger receptor-mediated phagocytosis in differentiated macrophage-like cells (dTHP-1) without a significant impact on the overall internalization efficiency. Taken together, this work demonstrates the disparate effects of a given protein corona on particle-cell interactions, highlighting the correlation between protein corona conformation in situ and relevant biological characteristics for biological functionalities.
246 citations
TL;DR: Its synthesis by normal mesenchymal cells and by malignant or transformed cells of both ectodermal and endodermal origin suggests a general role in cell function that is independent of transformation.
246 citations
TL;DR: In this review paper, a variety of critical factors in the PLGA microsphere formulation which might affect the release kinetic rate as well as the protein stability will be examined in detail.
Abstract: In this review paper, a variety of critical factors in the PLGA microsphere formulation which might affect the release kinetic rate as well as the protein stability will be examined in detail. Three model proteins, carbonic anhydrase, bovine serum albumin, and lysozyme, which were encapsulated within PLGA microspheres by using the water/oil/water (W/O/W) double emulsion solvent evaporation method are used for the study.
245 citations
TL;DR: The need for a standard was eliminated in the biuret reaction for the estimation of protein in urine by use of a photoelectric calorimeter by diluting pooled blood serum with 0.85 per cent sodium chloride to give solutions of various proteins.
244 citations