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Bovine serum albumin

About: Bovine serum albumin is a research topic. Over the lifetime, 19981 publications have been published within this topic receiving 571291 citations. The topic is also known as: BSA.


Papers
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Journal ArticleDOI
TL;DR: The data reveal that the polyphenol structures significantly affect the binding process: the binding affinity generally decreases with glycosylation and reduced numbers of hydroxyl groups on the second aromatic ring.

211 citations

Journal ArticleDOI
TL;DR: This assay procedure, which avoids chromatography and uses only one organic solvent extraction had blank values which approached zero, and introduction of an additional thin layer chromatographic step was associated with ...
Abstract: A globulin fraction rich in bound testosterone was separated from serum by ammonium sulfate precipitation. Testosterone and related steroids were quantitatively extracted from this fraction with a benzene petroleum ether mixture and measured by radioimmunoassay using testosterone-3-tyrosine methyl ester-125I and a specific antiserum elicited against testosterone-3-oxime bovine serum albumin. This assay procedure, which avoids chromatography and uses only one organic solvent extraction had blank values which approached zero. The within and between assay error, measured as the coefficient of variation was 7.8% (n = 64) and 8.2% (n = 15), respectively. The minimum amount of hormone per sample which can be measured with good reliability is 0.17 ng. This provides sufficient hormone to account for losses during the procedure, and to allow quadruplicate samples for radioimmunoassay and an aliquot for determining recovery. Although introduction of an additional thin layer chromatographic step was associated with ...

211 citations

Journal ArticleDOI
TL;DR: This factor is found in mouse follicular fluid collected 6 h following human chorionic gonadotropin injection to stimulate ovulation, but not in unstimulated mice, supporting the possibility that this molecule or molecules may diffuse into follicular fluids after an ovulatory stimulus to act as structural linkers that ensure normal cumulus expansion, through stabilization of the cumulus extracellular matrix thus supporting the process of ovulation.

210 citations

Journal ArticleDOI
TL;DR: In this paper, a variety of recombinant core protein fragments were expressed in Escherichia coli, extracted from inclusion bodies, and renatured in the presence of bovine serum albumin, which was essential for obtaining functional activity.

209 citations

Book
08 Oct 2007
TL;DR: The Comet Test for environmental genotoxicity evaluation: a fluorescence microscopy application and the effect of the structure and the environment of a fluorophore on its absorption and fluorescence spectra are studied.
Abstract: 1. Absorption spectroscopy theory. 2. Determination of Calcofluor White Molar extinction coefficient value in absence and presence of alpha1-acid glycoprotein. 3. Determination of kinetic parameters of Lactate dehydrogenase (LDH). 4. Hydrolysis of p-nitropenyl-beta-D-galactoside with beta-galactosidase from E.coli. 5. Starch hydrolysis by amylase. 6. Detrermination of the pK of a dye. 7. Fluorescence spectroscopy principles. 8. Effect of the structure and the environment of a fluorophore on its absorption and fluorescence spectra. 9. Fluorophores characterization and importance in biology. 10. Fluorescence quenching. 11. Fluorescence polarization. 12. Interaction between ethidium bromide and DNA. 13. Lens culinaris agglutinin: dynamics and binding studies. 14. Forster energy transfer. 15. Binding of TNS on bovine serum albumin at pH 3 and pH 7. 16. Comet Test for environmental genotoxicity evaluation: a fluorescence microscopy application. 17. Questions and exercises

209 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023475
2022983
2021423
2020460
2019468
2018489