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Showing papers on "Bradford protein assay published in 1982"


Journal ArticleDOI
01 Mar 1982
TL;DR: The procedure worked out was found adequate for the determination of the protein content of microbial cells immobilized in synthetic and native polymer-gel-systems.
Abstract: An assay for the determination of the protein content of whole cells immobilized in cross-linked synthetic gels was developed. The assay is based on a three step procedure: a) methanol dehydration, b) protein extraction by 1.0 M alkali at 125°C c) colorimetric assay of the extracted protein according to Bradford's procedure (Bradford M. M. (1976), Anal. Biochem. 72:248–254). The procedure worked out was found adequate for the determination of the protein content of microbial cells immobilized in synthetic and native polymer-gel-systems.

30 citations


Journal ArticleDOI
TL;DR: Iradiation with light of different wavelengths (white, red and blue light, respectively) during the plant cultivation in steady state conditions do not influence the amino acid composition of the soluble proteins.

27 citations