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Bradford protein assay
About: Bradford protein assay is a research topic. Over the lifetime, 635 publications have been published within this topic receiving 239107 citations.
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TL;DR: The Bradford protein quantification assay is based on an absorbance shift in Coomassie brilliant blue G-250, and the addition of any amount of polyphenolic compounds increased the Bradford reactive fraction (BRF) of soil extract.
Abstract: The Bradford protein quantification assay is based on an absorbance shift in Coomassie brilliant blue G-250 (CBB). Samples extracted for glomalin, a protein produced by arbuscular mycorrhizal (AM) fungi, are quantified using the Bradford assay. CBB is known to react with polyphenolic substances, and co-extraction of glomalin and humic substances is known to occur. The effects of increasing concentrations polyphenolic compounds were measured. The addition of any amount of polyphenolic compounds increased the Bradford reactive fraction (BRF) of soil extract. Caution is required when interpreting BRF data, as comparison of BRF data from different studies or different field sites is problematic. The BRF may represent recalcitrant organic material in soil, though its relationship to AM fungi remains unclear.
97 citations
TL;DR: The mechanism of eosin binding to proteins, which varies with solution pH so that a wide range of protein concentrations can be estimated, is described and the reason for the higher absorbance of protein-eosin Y complex compared to that ofprotein-eOSin B complex is discussed.
90 citations
TL;DR: The main result is that polymer-caused perturbations of the Coomassie dye absorbance at the Bradford monitoring wavelength (595nm) can be identified and corrected by recording absorption spectra in the region of 350-850mm.
90 citations
Book•
24 May 2002
TL;DR: Fundamental techniques: Kjeld method quantitative amino acid analysis and combustion analysis, and the Udy methods the Bradford method - principles Bradford assay - applications.
Abstract: Fundamental techniques: Kjeld method quantitative amino acid analysis and combustion analysis. Copper binding methods: the alkaline copper reagent - biuret assay the Lowry method the bicinchoninic acid protein assay. Dye binding methods: the Udy methods the Bradford method - principles Bradford assay - applications. Immunological methods for protein speciation: immunological assay - general principles and the Agar diffusion assay speciation of meat proteins by enzyme-linked immunsorbent assay speciation of soya protein enzyme-linked immunoassay determination of trace protein allergens in foods. Protein nutrient value: biological and chemical tests for protein nutrient value effect of processing on protein nutrient value protein digestibility - corrected amino acid scores.
89 citations
TL;DR: A simple and rapid microassay for proteins utilizing the protein dye-staining procedure with a nitrocellulose (NC) filter is described, with good linearity between the optical density and the amount of protein obtained.
88 citations