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Bradford protein assay
About: Bradford protein assay is a research topic. Over the lifetime, 635 publications have been published within this topic receiving 239107 citations.
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TL;DR: In this article , a new and scalable method for the isolation of extracellular vesicles (EV) from Citrus lemon juice samples was developed, which included preliminary preconcentration of the sample using ultrafiltration (UF) followed by size-exclusion chromatography (SEC) purification and final preconcence of the eluates.
TL;DR: In this article, ZnSe-ferritin was synthesized in the cavity of the apoferritin from horse spleen and the reaction condition was optimized by adding tween 20 to avoid ferritin agglomeration.
Abstract: In biomineralization, self-organization of organic based templates provides scaffolding for the assembly of QDs materials. The host-guest relationship between these protein cages and the encapsulated material is based primarily on a complementary electrostatic interaction. Zinc selenide (ZnSe) were synthesized in the cavity of the apoferritin from horse spleen (HsAFr) and the reaction condition was optimized by adding tween 20 to avoid ferritin agglomeration. The obtained nanodots were characterized by TEM, and absorption measurements. In addition, the protein concentration of ZnSe-ferritin was precisely measured by the Bradford protein assay method. From the results, it was concluded that the ZnSe nanocrystals were successfully synthesized in the core of ferritin and it can be applied as a potential functional material such as transistors, biosensor materials or medical imaging.
TL;DR: Almost all transfer factors marketed in Mexico lack a labeling and health record that meets the official standards, and the chromatographic profiles showed differences in some TF.
Abstract: Background: The transfer factor (TF) is the dialyzable extract of leukocytes with cellular immunity transfer properties. Its use has spread in the treatment of a wide range of immunologic, infectious, and even oncological diseases. However, important aspects in their protein profile, component concentrations, and a well-defined action mechanism are not completely unknown. Objectives: To analyze the protein profiles of different transfer factors marketed in Mexico. Methods: 6 TF marketed in Mexico were obtained and analyzed, quantifying protein with thaze Bradford method, by high-performance liquid chromatography (HPLC), and polyacrylamide gel electrophoresis (SDS-PAGE). All samples were analyzed in duplicate. Results : The total protein concentrations of all TF analyzed are less than 0.2 mg/mL. The chromatographic profiles showed differences in some TF. The protein concentration was 6 to almost one thousand times lower compared to reports by some manufacturers. Conclusion: Almost all transfer factors marketed in Mexico lack a labeling and health record that meets the official standards.
Patent•
25 Apr 2013
TL;DR: A protein assay apparatus includes at least one light-emitting unit for generating a first light, a second light or a third light for BCA protein assay, Bradford protein assay or Lowry protein assay.
Abstract: A protein assay apparatus includes at least one light-emitting unit for generating a first light, a second light or a third light for BCA protein assay, Bradford protein assay or Lowry protein assay, and at least one light-sensing unit for sensing the absorbed light passed through the test sample to calculate the concentration of protein in the test sample. This design shortens the protein examination time and greatly reduces the protein examination cost.
01 Mar 2019
TL;DR: In this article, the feasibility of neutron irradiation as a method for reducing milk allergens was analyzed and the results strongly indicate that the allergenicity of milk proteins can be reduced by fast neutron irradiations in an inverse exponential manner.
Abstract: This experiment was conducted to analyze the feasibility of neutron irradiation as a method for reducing milk allergens. In a temperature-controlled environment, whole milk was irradiated with 4 MeV fast neutron irradiation from a 5 Ci Pu-Be source. After irradiation, the samples were treated with Bradford reagent as an assay. The final concentrations from the Bradford Assay indicated protein degradation rather than denaturation leading to further testing with discontinuous polyacrylamide gel electrophoresis. The gel electrophoresis results determined that most proteins had been degraded into unidentifiable proteins components. The remaining main allergenic proteins, alpha-casein and beta-lactoglobulin, decreased by a factor of 3 and 13 respectively after 30 hours of irradiation in an inverse exponential manner. The results strongly indicate that the allergenicity of milk allergens can be reduced by fast neutron irradiation.