Topic
Bradford protein assay
About: Bradford protein assay is a research topic. Over the lifetime, 635 publications have been published within this topic receiving 239107 citations.
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TL;DR: A new protein determination method by enhanced Rayleigh light scattering (RLS) technique has been developed and the results of determination for human serum samples were comparable to those obtained by Bradford method.
49 citations
TL;DR: The automated system has been demonstrated for the successive assay of protein and glucose in urine samples taken from diabetic disease patients, with good agreement with the other methods, an alternative automation for screening for diabetic diagnosis.
49 citations
TL;DR: A semi-automated modification of the protein determination procedure of O. Randall (1951), carried out in 96-well microtest plates on protein solutions of 50 microliter or less, and can detect less than 0.5 micrograms of protein (equivalent to about 10 cultured cells).
48 citations
TL;DR: The developed enzyme-assisted extraction method has shown a faster extraction, higher recovery and reduced solvent usage with respect to the use of the non-enzymatic methods described in literature.
48 citations
TL;DR: This report assesses how the protein incubated in sample tubes may be lost due to adsorption and a model system to evaluate these phenomena is proposed.
Abstract: The non-specific loss of protein analytes can have a major effect on assay results particularly where the concentrations of such analytes are extremely low and the matrix is complex. This report assesses how the protein incubated in sample tubes may be lost due to adsorption. Use of proteins, such as bovine serum albumin (BSA), may be used to pre-treat tubes to reduce such losses. However, such losses may also be associated with structural perturbations leading to changes in immunogenicity (as a result of alterations in specific epitope-related conformations). This can lead to erroneous results or lack of comparability with a range of methodologies such as the bicinchoninic protein assay and immunoassays or when surface plasmon resonance (SPR)-based approaches are used. A model system to evaluate these phenomena is proposed.
48 citations