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Bradford protein assay
About: Bradford protein assay is a research topic. Over the lifetime, 635 publications have been published within this topic receiving 239107 citations.
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TL;DR: A novel protein assay applying the 4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic acid dipotassium salt (BisANS) fluorescent dye has several advantageous properties related to protein detection: good solubility in water, high photostability at adequate pH, quick interaction kinetics with proteins and no exclusionary sensitivity to the chelator, detergent and inhibitor ingredients.
Abstract: In many biology- and chemistry-related research fields and experiments the quantification of the peptide and/or protein concentration in samples are essential. Every research environment has unique requirements, e.g. metal ions, incubation times, photostability, pH, protease inhibitors, chelators, detergents, etc. A new protein assay may be adequate in different experiments beyond or instead of the well-known standard protocols (e.g. Qubit, Bradford or bicinchoninic acid) in related conceptions. Based on our previous studies, we developed a novel protein assay applying the 4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic acid dipotassium salt (BisANS) fluorescent dye. This molecule has several advantageous properties related to protein detection: good solubility in water, high photostability at adequate pH, quick interaction kinetics (within seconds) with proteins and no exclusionary sensitivity to the chelator, detergent and inhibitor ingredients. The protocol described in this work is highly sensitive in a large spectrum to detect protein (100-fold diluted samples) concentrations (from 0.28 up to more than 100 μg/mL). The BisANS protein assay is valid and applicable for quantification of the amount of protein in different biological and/or chemical samples.
11 citations
TL;DR: In this article, a reliable quantification method of Glomalin-related soil protein (GRSP) from forest soil, very rich in organic matter and therefore in colour, was proposed.
11 citations
TL;DR: A fluorometric micro protein assay based on fluorescamine-labelling of homogeneous proteins in solution has been developed which is capable of accurately quantitating as little as 25 ng protein at a concentration of 1.25 micrograms/ml.
11 citations
TL;DR: The effect of pH 7 may be used to determine the possible extent of hydrogen peroxide interference after treatment with catalase, and may interfere with protein estimations at alkaline pH.
11 citations
TL;DR: The procedures described herein require only minute quantities of sample and are sufficiently simple and rapid to be used as screening procedures and are more sensitive than many of the more commonly used diagnostic tests for urine protein and glucose.
11 citations