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Bradford protein assay
About: Bradford protein assay is a research topic. Over the lifetime, 635 publications have been published within this topic receiving 239107 citations.
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TL;DR: Bradford assay is presented as an alternative to Lowry test to quantify hair damage during combing or brushing and shows that virgin hair releases less protein than bleached hair.
Abstract: This study intends to present Bradford assay as an alternative to Lowry test to quantify hair damage during combing or brushing. The protocol involves collecting hair fragments that are chipped away from hair during these abrasive treatments and quantitatively measuring the amount of protein using an analytical procedure to detect low amounts of proteins. This protein determination method involves the binding of Coomassie Brilliant Blue G-250 to hair protein (keratin). It is quite rapid and sensitive and less prone to interferences as the standard Lowry procedure. The latter is subject to interference from compounds such as lipids, cationic surfactants and EDTA, which are ingredients commonly used in hair care formulations and may lead to a false positive result. These drawbacks should be eliminated when using the so called Bradford method for hair protein quantitation. Our studies showed reproducible results for human hair protein and the developed color was stable for up to one hour. The data also show that virgin hair releases less protein than bleached hair. The amount detected for the former after combing ranges from 0.875 to 1.03 mg/g of hair and 4.85 to 5.35 mg/g of hair for the latter.
7 citations
TL;DR: It is suggested that rapid diagnosis of immunoglobulins is possible using the concept of protein corona formation ex vivo, and it is predicted that the changes in secondary structure of primary corona determine the overall signature of surface binding proteins in PC.
7 citations
TL;DR: The modified method is based on fluorescence quenching of eosin Y by protein and modifies a method originally published by Hiraoka and Glick and should be useful not only where microgram quantities of protein must be measured, but also with automated equipment or in cases where the Folin-Ciocalteau method cannot be employed due to presence of interfering substances.
7 citations
TL;DR: It is found that SDS acts as an effective reagent for protein blotting onto a hydrophobic membrane of polyvinylidene difluoride with a manifold dot-blot apparatus and bolts stained with a pyrogallol red-molybdate complex (Pyromolex) reagent unreactive to the membrane allowed a precise protein determination without significant interference of materials, especially detergents in the sample solution.
7 citations
01 Jan 2013
TL;DR: The results suggest that the local dye was more sensitive than biuret reagent for assaying proteins since the bovine serum albumin was diluted 100 times before the local dyed could be used for the assay.
Abstract: A new colorimetric method for the determination of proteins was investigated using the local dye "Uri isi". Serial dilution of a solution of bovine serum albumin (BSA) was made. Protein assay was carried out photo-metrically with BSA using both the biuret reagent and the local dye. The results showed that the optical density decreased from 1.269 to 0.189 with an increase in the concentration of protein in the case of biuret reagent and also decreased from 0.276 to 0.174 with increase in the concentration of protein in the case of the local dye. The results suggest that the local dye was more sensitive than biuret reagent for assaying proteins since the bovine serum albumin was diluted 100 times before the local dye could be used for the assay.
7 citations