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Bradford protein assay
About: Bradford protein assay is a research topic. Over the lifetime, 635 publications have been published within this topic receiving 239107 citations.
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TL;DR: In this paper, a microfluidic open circuit potential label-free protein assay was developed for the reagentless quantification of C-reactive protein (CRP), a model protein target, and further utilized to assess target-receptor binding kinetics.
Abstract: A microfluidic open circuit potential label-free protein assay was developed for the reagentless quantification of C-reactive protein (CRP), a model protein target, and further utilized to assess target-receptor binding kinetics. Generated sensors have very high baseline stabilities (<1% change in 100 min) and high levels of selectivity in complex media. Real-time assays are fast (<20 min), of high sensitivity (1 ng/mL limit of detection for CRP in serum), and resolve kinetic and thermodynamic characteristics that correlate well with those resolved optically. The assay shows excellent correlation with an enzyme-linked immunosorbent assay analysis of patient samples. The methodology has value in potentially underpinning a low-cost, rapid, and sensitive single-step biomarker quantification.
5 citations
Journal Article•
TL;DR: MPC is a method of simplicity, rapidity and sensitivity for protein quantification assay especially suitable for the determination of large number of microliter samples.
Abstract: AIM To develop a rapid and sensitive method of protein quantification assay. METHODS Protein content of bovine serum albumin (BSA) and mice liver homogenate was determinated by microplate colormetric (MPC) in which coomassie brilliant blue (CBB) is used as color reagent. RESULTS Detecting limit of MPC was lower (0.63 μg) than that of macro colormetric (MC). MPC had advantage of rapidity and lesser volume of sample (a few microliter). CONCLUSION MPC is a method of simplicity, rapidity and sensitivity for protein quantification assay especially suitable for the determination of large number of microliter samples.
5 citations
TL;DR: The variation in the sensitivity of proteins to some commonly used protein assay procedures was estimated by a calculation of the ideal titer per unit weight of protein for a sample of 350 proteins.
5 citations
TL;DR: The applicability of the Folin-Lowry protein assay to the determination of protein contamination of DNA was investigated with respect to its sensitivity and to the color contribution of possible degradative contaminants.
Abstract: The applicability of the Folin-Lowry protein assay to the determination of protein contamination of DNA was investigated with respect to its sensitivity and to the color contribution of possible degradative contaminants. Quantities of protamine and histone on the order of 10 to 15 g may be detected by this method, which may be applied to preparations of DNA from which phenol, bases, nucleosides, and nucleotides have been removed. (auth)
5 citations
5 citations