Showing papers on "Brilliant green published in 2002"

Decolorization of triphenylmethane and azo dyes by Citrobacter sp.

Abstract: A Citrobacter sp., isolated from soil at an effluent treatment plant of a textile and dyeing industry, decolorized several recalcitrant dyes except Bromophenol Blue. More than 90% of Crystal Violet and Methyl Red at 100 μM were reduced within 1 h. Gentian Violet, Malachite Green and Brilliant Green lost over 80% of their colors in the same condition, but the percentage decolorization of Basic Fuchsin and Congo Red were less than the others, 66 and 26%, respectively. Decolorization of Congo Red was mainly due to adsorption to cells. Color removal was optimal at pH 7–9 and 35–40 °C. Decolorization of dyes was also observed with extracellular culture filtrate, indicating the color removal by enzymatic biodegradation.

Degradation of textile dyes mediated by plant peroxidases.

Abstract: The peroxidase enzyme from the plants Ipomea palmata (1.003 IU/g of leaf) and Saccharum spontaneum (3.6 IU/g of leaf) can be used as an alternative to the commercial source of horseradish and soybean peroxidase enzyme for the decolorization of textile dyes, mainly azo dyes. Eight textiles dyes currently used by the industry and seven other dyes were selected for decolorization studies at 25-200 mg/L levels using these plant enzymes. The enzymes were purified prior to use by ammonium sulfate precipitation, and ion exchange and gel permeation chromatographic techniques. Peroxidase of S. spontaneum leaf (specific activity of 0.23 IU/mg) could completely degrade Supranol Green and Procion Green HE-4BD (100%) dyes within 1 h, whereas Direct Blue, Procion Brilliant Blue H-7G and Chrysoidine were degraded >70% in 1 h. Peroxidase of Ipomea (I. palmata leaf; specific activity of 0.827 U/mg) degraded 50 mg/L of the dyes Methyl Orange (26%), Crystal Violet (36%), and Supranol Green (68%) in 2-4 h and Brilliant Green (54%), Direct Blue (15%), and Chrysoidine (44%) at the 25 mg/L level in 1 to 2 h of treatment. The Saccharum peroxidase was immobilized on a hydrophobic matrix. Four textile dyes, Procion Navy Blue HER, Procion Brilliant Blue H-7G, Procion Green HE-4BD, and Supranol Green, at an initial concentration of 50 mg/L were completely degraded within 8 h by the enzyme immobilized on the modified polyethylene matrix. The immobilized enzyme was used in a batch reactor for the degradation of Procion Green HE-4BD and the reusability was studied for 15 cycles, and the half-life was found to be 60 h.

Biodecolourization of azo and triphenylmethane dyes by Dichomitus squalens and Phlebia spp.

Abstract: Nine white-rot fungal strains were screened for biodecolourization of brilliant green, cresol red, crystal violet, congo red and orange II. Dichomitus squalens, Phlebia fascicularia and P. floridensis decolourized all of the dyes on solid agar medium and possessed better decolourization ability than Phanerochaete chrysosporium when tested in nitrogen-limited broth medium.

Ultrafast Excited State Deactivation of Triphenylmethane Dyes

Abstract: We have carried out ultrafast pump−probe measurement of four TPM dyes, malachite green (MG), brilliant green (BG), crystal violet (CV), and ethyl violet (EV), with a time resolution of 30 fs The pump−probe signal showed that solvent dependence arose first in the femtosecond time regime, eg, the decay of n-butanol solution was clearly slower than the methanol solution just 50 fs after the initial photoexcitation The signal decays in a multiexponential manner and the slower components showed stronger linear dependence on the solvent viscosity than did the faster components We have also carried out temperature-dependent measurement of ethanol solution and calculated the activation energies from the Arrhenius plots of each components The activation energies and effective volumes were larger for slower decays The activation energy of the viscosity of ethanol was larger than that of the decay components of TPM dyes These observations are explained with a combined effect of microviscosity and intramolecu

New visualizing reagents for selected phenolic drugs investigated by thin layer chromatography

Abstract: Thirteen new visualizing reagents have been used to detect 13 phenolic drugs following thin layer chromatography on silica gel layers. Limits of detection (detectability), detectability index, and broadening index were determined for these drugs following use of these visualizing reagents. Aniline blue and brilliant green were the best and most universal visualizing reagents for the phenolic drugs investigated. Densitograms of selected phenolic drugs after spraying with aniline blue and brilliant green are presented.