Brilliant green

About: Brilliant green is a research topic. Over the lifetime, 627 publications have been published within this topic receiving 12495 citations.

Detection of Verocytotoxygenic Escherichia coli (VTEC) from Brilliant Green Lactose Bilebroth (BGLB) by Polymerase Chain Reaction (PCR)

Abstract: Verocytotoxygenic Escherichia coli (VTEC) is pathogenic bacteria for human and associated with dairy milk and dairy farm environment. VTEC isolation were carried out by inoculating in BGLB as enrichment media and then specified in Eosin Methylene Blue (EMB) agar. This study aim to identify VTEC isolated from BGLB using PCR method. Six VTEC sample were used in this study, and those samples were collected from : milk bulktank (n=1), hand swab before milking (n=1), and hand swab after milking (n=1), swab of milk filter (n=1), feces (n=1), and water from dairy drinking places (n=1). All samples were inoculated in BGLB, and then bacterial DNA were extracted. PCR amplification using specific primer of vt1 and vt2 targeting on.....gene and....gene, with expected band at 130 bp and 779 bp, respectively. Positive results were sequenced and then analyzed using BLAST. Our results showed two out of six were positive of VTEC. Keywords—VTEC, dairy farm, BGLB, PCR, primer vt1, and vt2.

Photodegradation of Brilliant Green Dye by a Zinc bioMOF and Crystallographic Visualization of Resulting CO2.

Abstract: We present a novel bio-friendly water-stable Zn-based MOF (1), derived from the natural amino acid L-serine, which was able to efficiently photodegrade water solutions of brilliant green dye in only 120 min. The total degradation was followed by UV-Vis spectroscopy and further confirmed by single-crystal X-ray crystallography, revealing the presence of CO2 within its channels. Reusability studies further demonstrate the structural and performance robustness of 1.

Synthesis of CTAB modified ferrite composite for the efficient removal of brilliant green dye

Abstract: In the present work, magnetic nickel ferrite (NiFe2O4) have been synthesised using low temperature combustion method and then modified with Cetyltrimethylammonium bromide (CTAB) to form a magnetic composite. Prepared composite (CTAB-NiFe2O4) was used as an adsorbent for removing brilliant green dye from aqueous solution. Adsorbent was investigated by using various techniques like Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, energy-dispersive spectra, thermogravimetric analysis and pH of point zero charge. Different parameters such as effect of contact time, pH, adsorbent dose and initial dye concentration were studied for the adsorption of dye. The optimum values observed were 170 minutes equilibrium time at pH 6 and adsorbent dose 0.6 g. Various adsorption kinetic models such as pseudo first-order kinetic model, pseudo second-order kinetic model and Elovich model were used to determine the nature of adsorption. Pseudo second-order kinetic model fitted better with higher R2 value which indicated that the adsorption was chemical in nature. Langmuir isotherm was best fitted to experimental data for the adsorption of brilliant green dye with maximum adsorption capacity ‘Qe’ 250 mg/g. It revealed that the adsorption in this study takes place on homogenous surface and follows monolayer pattern. Therefore, for the removal of Brilliant green dye from wastewater using composite (CTAB-NiFe2O4) can be considered as an effective adsorbent.

Isolation of increased mumbers of unclassified mycobacteria following decontamination of sputum with malachite green and crystal violet.

Abstract: The aniline dyes have been utilized in the primary isolation of 111 ycobacterium tuberculosis for the suppression in the culture medium of the growth of those' contaminants which have escaped the prior action of decontaminating agents such as antiformin, hydrochloric and sulfuric acids, ammonium carbonate, trisodium phosphate, and sodium hydroxide. That the aniline dyes possess bactericidal as well as bacteriostatic properties was revealed by the investigation of Churchman (1). In those studies it was observed that bacteria which had been exposed for various periods of time to crystal violet, malachite green, and brilliant green failed to grow when inoculated upon culture medium. While it was found that the gram-negative organisms \vere much more resistant to the dyes than the gram-positive bacteria, it was also observed that the mycobacteria were particularly resistant to the lethal action of the dyes. These findings served as the basis for the present investigation of the use of malachite green and crystal violet for decontamination of tuberculous sputum prior to culture.