Topic
Brilliant green
About: Brilliant green is a research topic. Over the lifetime, 627 publications have been published within this topic receiving 12495 citations.
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TL;DR: The dye is not decolorized when heated in distilled water or peptone, but is decolorizing by heating in glucose, glycine, or sodium dodecyl sulfate, showing that decolorization results from reaction with components of the medium.
Abstract: Antimicrobial activity of brilliant green dye in Trypticase soy broth (BBL) is reduced and ultimately destroyed by prolonged autoclaving at 121 C. Loss of antimicrobial activity is accompanied by decolorization of the dye. This is consistent with other evidence that antimicrobial activity of brilliant green resides in the colored dye ion. The dye is not decolorized when heated in distilled water or peptone, but is decolorized by heating in glucose, glycine, or sodium dodecyl sulfate, showing that decolorization results from reaction with components of the medium. To ensure optimal results, it is recommended that bacteriological media be sterilized by heat prior to addition of brilliant green dye.
12 citations
Journal Article•
TL;DR: Transition of the dye into the carbinol form is in water extremely slow, but is greatly accelerated in the presence of an organic phase, at least for malachite green and brilliant green, but not for crystal violet and pararosaniline, which explains the different action of the two categories of dyes on erythrocytes.
Abstract: The action of a series of triphenylmethane dyes on the membranes of two different cell types, yeast cells and erythrocytes, have been studied. The action of the dyes on yeast cells resembles the action of other positively charged bactericides. The sequence of the activity in the dye series is completely different for yeast cells and erythrocytes. Pararosaniline and crystal violet are much more active than malachite green and brilliant green on yeast cells, whereas the reverse sequence of activities applies in erythrocytes. The carbinol form of the dyes plays an important role as regards dye interaction with erythrocytes. Transition of the dye into the carbinol form is in water extremely slow, but is greatly accelerated in the presence of an organic phase, at least for malachite green and brilliant green, but not for crystal violet and pararosaniline. This explains the different action of the two categories of dyes on erythrocytes. Experiments with pure carbinol also support the hypothesis.
12 citations
TL;DR: In this paper, the authors describe the successful synthesis of a new magnetic nanocomposite and investigate simultaneous removal of 2 cationic dyes, brilliant green (BG) and malachite green (MG), from aqueous solutions.
Abstract: Here, we describe the successful synthesis of a new magnetic nanocomposite and investigate simultaneous removal of 2 cationic dyes, brilliant green (BG) and malachite green (MG), from aqueous solutions. The nanocomposite is characterized by scanning electron microscopy , energy‐dispersive X‐ray spectroscopy, thermal gravimetric analysis, and Fourier‐transform infrared spectrophotometry. The spectra of BG and MG in their mixture show severe overlapping. Therefore, partial least square (PLS) regression is applied for simultaneous determination of target dyes. The original and first derivative spectra of the binary mixtures are used to perform the optimization of the calibration matrices by the PLS method. According to statistical values, PLS on first derivative spectral data has given better results with respect to the original data. The coefficients of determination (R2) for the test data set were 0.998 and 0.9996; root mean square error of prediction values for BG and MG were 0.12 and 0.19, respectively. Furthermore, adsorption kinetics and effects of various variables such as solution pH, adsorbent amount, and initial dye concentration are investigated. Under optimized conditions (adsorbent mass, 0.08 g; solution pH, 8; and contact time, 60 min), equilibrium experimental data are represented by Langmuir, Freundlich, and Sips isotherms. Results briefly show that adsorption of dyes by nanocomposite obeys pseudo–second‐order kinetic model and Sips isotherm.
12 citations
TL;DR: The detoxification of the dilute phase was achieved, which indicated that the Dilute phase could be discharged without causing dye pollution, in triphenylmethane dye wastewater.
Abstract: The biological treatment of triphenylmethane dyes is an important issue. Most microbes have limited practical application because they cannot completely detoxicate these dyes. In this study, the extractive biodecolorization of triphenylmethane dyes by Aeromonas hydrophila DN322p was carried out by introducing the cloud point system. The cloud point system is composed of a mixture of nonionic surfactants (20 g/L) Brij 30 and Tergitol TMN-3 in equal proportions. After the decolorization of crystal violet, a higher wet cell weight was obtained in the cloud point system than that of the control system. Based on the results of thin-layer chromatography, the residual crystal violet and its decolorized product, leuco crystal violet, preferred to partition into the coacervate phase. Therefore, the detoxification of the dilute phase was achieved, which indicated that the dilute phase could be discharged without causing dye pollution. The extractive biodecolorization of three other triphenylmethane dyes was also examined in this system. The decolorization of malachite green and brilliant green was similar to that of crystal violet. Only ethyl violet achieved a poor decolorization rate because DN322p decolorized it via adsorption but did not convert it into its leuco form. This study provides potential application of biological treatment in triphenylmethane dye wastewater.
12 citations
TL;DR: In this paper, a sensitive extraction-spectrophotometric method of the determination of osmium, taking advantage of the ion-associate of the chloride Osmium anion with brilliant green has been developed.
12 citations