Showing papers on "Brucine published in 1988"
TL;DR: The Na+-independent binding of [3H]β-alanine to rat brain stem plus spinal cord was reinvestigated, in order to study in more detail the characteristics of previously described β-alanines binding processes.
Abstract: The Na+-independent binding of [3H]β-alanine to rat brain stem plus spinal cord was reinvestigated, in order to study in more detail the characteristics of previously described β-alanine binding processes. Binding was absent when amino acid-free postnuclear supernatants or crude synaptic membranes were used. Experiments performed with several other Na+-free preparations showed a sole binding component, irrespective of the preparation used. Biochemical characterization of this Na+-independent binding, using frozen/thawed/washed synaptosomal-mitochodrial fractions, showed that binding reached a plateau between 7 min and 13 min, increasing thereafter. Binding was linear with fraction protein over a range of 200–415 μg/ml incubation medium. Binding was completely inhibited by glycine, alanine, α-aminobutyric acid, β-aminoisobutyric acid, hypotaurine and strychnine, and to a lesser extent by 2,2-dimethyl-β-alanine, brucine and gelsemine. It was insensitive to taurine, γ-aminobutyric acid (GABA), 2-guanidinoethanesulfonic acid (GES), carnosine, and bicuculline methiodide. Binding was reversible, saturable (KD 20 μM), and heat sensitive.
8 citations
TL;DR: The bark of Strychnos potatorum, is used as an adulterant of S. nux-vomica, which is highly poisonous, containing the alkaloids strychnine and brucine, whereas the former is nonpoisonous.
Abstract: The bark of Strychnos potatorum L. (Loganiaceae), is used as an adulterant of S. nux-vomica L. The latter is highly poisonous, containing the alkaloids strychnine and brucine, whereas the former is nonpoisonous. The two barks could be distinguished by pharmacognostic evaluation.
4 citations
TL;DR: In this paper, host-guest complexation method was applied to isolation of caffeine, nicotine, and cholesterol from tea and tabaco leaves, respectively, and gallstone, respectively.
Abstract: Application of host–guest complexation method to isolation of caffeine, nicotine, and cholesterol from tea and tabaco leaves, and gallstone, respectively, has been reported. Separations of strychnine and brucine, and sparteine and brucine by the same method were also reported.
3 citations
TL;DR: The results indicate that a rapid and accurate estimation of the utility of activated charcoal as an antidote for drugs and toxic substances can be obtained from a single chromatographic run of the test compound.