Showing papers on "Brucine published in 2003"
TL;DR: The results suggest that central and peripheral mechanism are involved in the pain modulation and anti-inflammation effects of brucine and Brucine N-oxide, biochemical mechanisms of bru cine and brucines N -oxide are different even though they are similar in chemical structure.
182 citations
TL;DR: A quantitative analysis using (1)H-N MR (Q-NMR) has been developed for the determination of strychnine and brucine in Strychnos nux-vomica seeds and stems.
Abstract: A quantitative analysis using (1)H-NMR (Q-NMR) has been developed for the determination of strychnine and brucine in Strychnos nux-vomica seeds and stems. The advantages of the method are that no reference alkaloids are needed for calibration curves, the quantification could be directly realized on a crude extract, strychnine and brucine could easily be distinguished, an overall profile of the preparation (including non alkaloid compounds) could be directly obtained, and a very significant time-gain could be achieved, in comparison to conventional HPLC methods, for instance.
42 citations
TL;DR: A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of strychnine and brucine is established and can be separated within 5 min and quantified with high sensitivity.
Abstract: A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of strychnine and brucine is established. After optimization of the separation and concentration conditions, the two alkaloids can be separated within 5 min and quantified with high sensitivity (The detection limits were 1.0 ng mL−1 for strychnine and 1.4 ng mL−1 for brucine). The method was useful for qualitative and quantitative analysis of strychnine and brucine in Strychnos nux-vomica L with recovery of 105.1% for strychnine and 98.4% for brucine.
22 citations
Journal Article•
TL;DR: The RP-HPLC method is a good reference for pharmacokinetics in human bodies and can be used to study the pharmacokinetic process about the concentration in rat plasma of the alkaloids from processed seeds of Strychnos nux-vomica with RP- HPLC method.
Abstract: AIM To study the pharmacokinetic process about the concentration in rat plasma of the alkaloids from processed seeds of Strychnos nux-vomica with RP-HPLC method. METHODS Hypersil BDS C18 column was used and the mobile phase consisted of acetonitrile-water at the flow rate of 0.8 mL.min-1. The UV detection wave length was 254 nm. RESULTS The concentration-time data of strychnine, brucine, strychnine N-oxide and brucine N-oxide were all in accordance with an open two-compartment model after i.v. alkaloids. Their parameters were as follows: T1/2 alpha were (8 +/- 5), (4 +/- 3), (6.2 +/- 1.7) and (3.0 +/- 0.8) min, T1/2 beta were (262 +/- 125), (416 +/- 131), (285 +/- 50) and (342 +/- 141) min, CL were (17 +/- 4), (21 +/- 12), (1.9 +/- 1.8) and (2.8 +/- 1.1) mL.min-1, Vc were (1.4 +/- 0.5), (1.7 +/- 1.1), (0.24 +/- 0.16) and (0.23 +/- 0.06) L.kg-1, Vd were (6.0 +/- 1.2), (12 +/- 7), (0.8 +/- 0.6) and (1.5 +/- 0.6) L.kg-1, AUC were (57,578 +/- 25,578), (35,240 +/- 15,616), (93,088 +/- 22,375) and (177,712 +/- 120,110) h.microgram.L-1, respectively. CONCLUSION The method is a good reference for pharmacokinetics in human bodies.
13 citations
TL;DR: Bisquaternary dimers of strychnine and brucine were synthesized and their allosteric effect on muscarinic acetylcholine M(2) receptors was examined, which indicated ternary complex formation.
11 citations
TL;DR: In this article, a rapid and simple spectrophotometric procedure is described for the determina- tion of ajmaline and brucine, which is based on the development of blue coloured product due to reduction of tungstate and/or molybdate in Folin Ciocalteu's reagent (FCR) in alkaline medium.
Abstract: A rapid and simple spectrophotometric procedure is described for the determina- tion of ajmaline and brucine. The method is based on the development of blue coloured product due to reduction of tungstate and/or molybdate in Folin Ciocalteu's reagent (FCR) by ajmaline and brucine in alkaline medium. The colour is stable for more than 48 h. The chromogenic reaction hasmax at 540 nm with molar absorptivity 1.64 10 4 and 2.37 10 3 l mol -1 cm -1 in the Beer's law range 1-8 gm l -1 and 10-100 gm l -1 for ajmaline and brucine, respectively.
8 citations
TL;DR: Results indicate that the reaction of nitrate ion with brucine and 3-methyl-2-benzothiazolinone hydrazone hydrochloride in sulfuric acid medium to yield a violet-colored product which is stable for over two days is highly sensitive.
Abstract: A modified and highly sensitive spectrophotometric method for the determination of nitrate in trace quantities in environmental samples is described. The method is based on the reaction of nitrate ion with brucine and 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) in sulfuric acid medium to yield a violet-colored product which is stable for over two days. The optimum photometric range for the determination of nitrate is 0.04-0.16 microg cm(-3) and the Sandell's sensitivity being 0.000279 microg cm(-2). The proposed method is applied to various water samples and the results indicate that the reaction is highly sensitive than the original brucine method.
4 citations
Journal Article•
TL;DR: In this article, a high performance liquid chromatographic (HPLC) method was developed for the multaneous determination of brucine and strychnine in human blood, and the recoveries obtained from spiked blood for the t wo alkaloids were more than 90%.
1 citations