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Brucine

About: Brucine is a research topic. Over the lifetime, 586 publications have been published within this topic receiving 6866 citations. The topic is also known as: 10,11-dimethoxy strychnine.


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Journal ArticleDOI
TL;DR: The results of this study indicated that transdermal administration might be beneficial for the sustained efficacy and reduced toxicity of brucine.

16 citations

Journal ArticleDOI
TL;DR: The developed liquid chromatography-electrospray ionization-ion trap mass spectrometry method provided high sensitivity and selectivity for the determination of poisonous alkaloids and their major metabolites and can be applied in the determined of samples in forensic and clinically toxicological cases.
Abstract: A liquid chromatography –electrospray ionization– ion trap mass spectrometry (LC –ESI–ITMS) method was developed for the simultaneous analysis of strychnine, brucine and their major metabolites. Strychnine and brucine were individually incubated with rat liver S9 fraction. The incubation samples were pooled together and analyzed with LC–ESI –ITMS in positive ion and full-scan detection mode. The calibration curves of strychnine and brucine in rat liver showed good linearity in ranges of 0.020 to 8.0 mg/mL for strychnine and 0.020 to 8.5 mg/mL for brucine. The limits of detections were both 0.008 mg/mL and the recoveries were 88.3 and 83.2% for strychnine and brucine, respectively. Two metabolites were identified as strychnine N-oxide and brucine N-oxide by comparing the molecular mass, retention time, full-scan mass spectra, tandem MS and MS 3 spectra with those of strychnine and brucine. The developed method provided high sensitivity and selectivity for the determination of poisonous alkaloids and their major metabolites and can be applied in the determination of samples in forensic and clinically toxicological cases.

15 citations

Journal ArticleDOI
TL;DR: A simple, rapid, and sensitive liquid chromatography-mass spectrometric method was developed and validated for simultaneous determination of main bioactive ingredients in liquorice and Semen Strychni in rat plasma and the results show that this method is sensitive, accurate and robust for biological matrix analysis.
Abstract: Semen Strychni is known for its treatment of rheumatic arthritis with a low therapeutic index. Licorice contributes a lot in herb detoxification according to the traditional Chinese medicine theory. A simple, rapid and sensitive liquid chromatography–mass spectrometric method was developed and validated for simultaneous determination of main bioactive ingredients in licorice and Semen Strychni in rat plasma. Using moclobemide and cyproterone acetate as the internal standards, the analytes were pretreated via protein precipitation with methanol. An Ultimate AQ-C18 column (3.0 μm, 3.0×100 mm) was employed for chromatographic separation, combining with gradient elution. The mobile phase consisted of 0.07% formic acid and 0.12% ammonium acetate in aqueous phase (A) and acetonitrile in organic phase (B). The elution program was as follows: 0–0.5 min, 20% B; 0.5–1 min, 20–60% B; 1–7 min, 60–85% B; and 7–7.5 min, returned to 20% B, then continued to 12 min. Selected reaction monitoring was performed in both positive and negative ESI. Positive mode was adopted for detection of strychnine, brucine and moclobemide, while negative mode was used for glycyrrhizic acid, glycyrrhetinic acid, liquiritigenin, isoliquiritigenin, liquiritin and cyproterone acetate. The method was validated for specificity, linearity, matrix effect, recovery, precision, accuracy and stability. The results show that this method is sensitive, accurate and robust for biological matrix analysis. Moreover, the proposed method was applied to a pharmacokinetic study in Sprague-Dawley rats for investigating the mechanism of which liquorice detoxifies Semen Strychni.

15 citations

Journal ArticleDOI
TL;DR: Crystals of brucine and its 2-propanol solvate dihydrate exhibit similar monolayer sheet packing, whereas crystals of the acetone Solvate adopt a different mode of packing, as brucines pillars.
Abstract: The crystal structures of brucine (2,3-di­methoxy­strychnidin-10-one), C23H26N2O4, brucine acetone solvate, C23H26N2O4·C3H6O, and brucine 2-propanol solvate dihydrate, C23H26N2O4·C3H7O·2H2O, have been determined. Crystals of brucine and its 2-propanol solvate dihydrate exhibit similar monolayer sheet packing, whereas crystals of the acetone solvate adopt a different mode of packing, as brucine pillars. The solvent appears to control the brucine self-assembly on the basis of common donor–acceptor properties of the surfaces.

15 citations

Journal ArticleDOI
TL;DR: It was concluded that the anions play an important role in the distribution of the alkaloids between the organic and aqueous phase and a solvent system is proposed consisting of a pH = 4 Mcllvaine buffer containing 1 M sodium perchlorate - methanol - chloroform.
Abstract: The separation of the alkaloids strychnine, brucine, quinine, dihydroquinine, and berberine by using pH-gradients in Droplet Counter-Current Chromatography was studied. Gradients from pH 8-2.5 resulted in separation of the alkaloids mentioned. It was further concluded that the anions play an important role in the distribution of the alkaloids between the organic and aqueous phase. Based on these conclusions a solvent system is proposed consisting of a pH = 4 Mcllvaine buffer containing 1 M sodium perchlorate - methanol - chloroform (3:5:5), using the aqueous phase as mobile phase (ascending mode) for the separation of the alkaloids. By using a gradient with a decreasing concentration of the counterion, the retention time of the alkaloids can be reduced.

15 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20235
202224
202117
20208
201912
201812