Topic
Brucine
About: Brucine is a research topic. Over the lifetime, 586 publications have been published within this topic receiving 6866 citations. The topic is also known as: 10,11-dimethoxy strychnine.
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TL;DR: In this paper, a method for the determination of brucine, strychnine and salvianolic acid B in Biqi capsule, a Inertsil ODS-3(250 mm×46 mm,5 μm)column was used with acetonitrile and 10 mmol/L Sodium 1-hexanesulfonate phosphate-buffered saline(pH 286)(22∶78,v/v) as the mobile phase The flow rate was 10 mL/min ; the detective wave was 254 nm and the column temperature was set at 25 �
2 citations
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TL;DR: This validated method was successfully applied to bidirectional transport study of moclobemide blood-brain barrier permeability and was validated from LLOQ to 1980 ng/mL with a coefficient of determination greater than 0.999.
Abstract: A simple and sensitive analytical method based on ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been developed for determination of moclobemide in human brain cell monolayer as an in vitro model of blood-brain barrier. Brucine was employed as the internal standard. Moclobemide and internal standard were extracted from cell supernatant by ethyl acetate after alkalinizing with sodium hydroxide. The UPLC separation was performed on an Acquity UPLC(TM) BEH C18 column (50 × 2.1 mm, 1.7 µm, Waters, USA) with a mobile phase consisting of methanol-water (29.5:70.5, v/v); the water in the mobile phase contained 0.05% ammonium acetate and 0.1% formic acid. Detection of the analytes was achieved using positive ion electrospray via multiple reaction monitoring mode. The mass transitions were m/z 269.16 → 182.01 for moclobemide and m/z 395.24 → 324.15 for brucine. The extraction recovery was 83.0-83.4% and the lower limit of quantitation (LLOQ) was 1.0 ng/mL for moclobemide. The method was validated from LLOQ to 1980 ng/mL with a coefficient of determination greater than 0.999. Intra- and inter-day accuracies of the method at three concentrations ranged from 89.1 to 100.9% for moclobemide with precision of 1.1-9.6%. This validated method was successfully applied to bidirectional transport study of moclobemide blood-brain barrier permeability.
2 citations
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TL;DR: In this paper, a color reaction was developed for Spectrophotometric determination of manganese in minute quantities and the maximum tolerable limit of other cations and anions was reported.
Abstract: Manganese solution in sodium hydroxide when mixed with brucine followed by HCl produces pink color having 1μg/ml as visual limit of identification and maximum absorbance at 475 nm. This color reaction has been developed for the Spectrophotometric determination of manganese in minute quantities. The maximum tolerable limit of other cations and anions is reported.
2 citations
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TL;DR: An easy and rapid method based on hydrophilic-interaction chromatography has been developed and validated for measurement of two major alkaloids, strychnine and brucine, in Strychnos nux-vomica plant seeds and preparations as mentioned in this paper.
Abstract: An easy and rapid method based on hydrophilic-interaction chromatography has been developed and validated for measurement of two major alkaloids, strychnine and brucine, in Strychnos nux-vomica plant seeds and preparations. The seeds were processed in different ways. Separation was performed at ambient temperature on a carbohydrate column with acetonitrile–water 90:10 (v/v) as mobile phase at a flow rate of 1.0 mL min−1. Detection was at 254 nm. Calibration plots were linear over the range 0.1–200 μg mL−1 for both compounds. Average recoveries of strychnine and brucine from spiked samples were 99.9 and 98.5%, respectively.
2 citations