scispace - formally typeset
Search or ask a question
Topic

Brucine

About: Brucine is a research topic. Over the lifetime, 586 publications have been published within this topic receiving 6866 citations. The topic is also known as: 10,11-dimethoxy strychnine.


Papers
More filters
Journal Article
TL;DR: In this paper, a method for the determination of brucine, strychnine and salvianolic acid B in Biqi capsule, a Inertsil ODS-3(250 mm×46 mm,5 μm)column was used with acetonitrile and 10 mmol/L Sodium 1-hexanesulfonate phosphate-buffered saline(pH 286)(22∶78,v/v) as the mobile phase The flow rate was 10 mL/min ; the detective wave was 254 nm and the column temperature was set at 25 �

2 citations

Journal ArticleDOI
Dai Li-Bo1, Yan Miao1, Li Huande1, Fang Pingfei1, Wang Feng1, Deng Yang1 
TL;DR: This validated method was successfully applied to bidirectional transport study of moclobemide blood-brain barrier permeability and was validated from LLOQ to 1980 ng/mL with a coefficient of determination greater than 0.999.
Abstract: A simple and sensitive analytical method based on ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been developed for determination of moclobemide in human brain cell monolayer as an in vitro model of blood-brain barrier. Brucine was employed as the internal standard. Moclobemide and internal standard were extracted from cell supernatant by ethyl acetate after alkalinizing with sodium hydroxide. The UPLC separation was performed on an Acquity UPLC(TM) BEH C18 column (50 × 2.1 mm, 1.7 µm, Waters, USA) with a mobile phase consisting of methanol-water (29.5:70.5, v/v); the water in the mobile phase contained 0.05% ammonium acetate and 0.1% formic acid. Detection of the analytes was achieved using positive ion electrospray via multiple reaction monitoring mode. The mass transitions were m/z 269.16 → 182.01 for moclobemide and m/z 395.24 → 324.15 for brucine. The extraction recovery was 83.0-83.4% and the lower limit of quantitation (LLOQ) was 1.0 ng/mL for moclobemide. The method was validated from LLOQ to 1980 ng/mL with a coefficient of determination greater than 0.999. Intra- and inter-day accuracies of the method at three concentrations ranged from 89.1 to 100.9% for moclobemide with precision of 1.1-9.6%. This validated method was successfully applied to bidirectional transport study of moclobemide blood-brain barrier permeability.

2 citations

Journal ArticleDOI
TL;DR: In this paper, a color reaction was developed for Spectrophotometric determination of manganese in minute quantities and the maximum tolerable limit of other cations and anions was reported.
Abstract: Manganese solution in sodium hydroxide when mixed with brucine followed by HCl produces pink color having 1μg/ml as visual limit of identification and maximum absorbance at 475 nm. This color reaction has been developed for the Spectrophotometric determination of manganese in minute quantities. The maximum tolerable limit of other cations and anions is reported.

2 citations

Journal ArticleDOI
TL;DR: An easy and rapid method based on hydrophilic-interaction chromatography has been developed and validated for measurement of two major alkaloids, strychnine and brucine, in Strychnos nux-vomica plant seeds and preparations as mentioned in this paper.
Abstract: An easy and rapid method based on hydrophilic-interaction chromatography has been developed and validated for measurement of two major alkaloids, strychnine and brucine, in Strychnos nux-vomica plant seeds and preparations. The seeds were processed in different ways. Separation was performed at ambient temperature on a carbohydrate column with acetonitrile–water 90:10 (v/v) as mobile phase at a flow rate of 1.0 mL min−1. Detection was at 254 nm. Calibration plots were linear over the range 0.1–200 μg mL−1 for both compounds. Average recoveries of strychnine and brucine from spiked samples were 99.9 and 98.5%, respectively.

2 citations


Network Information
Related Topics (5)
Acetic acid
33.9K papers, 458.3K citations
74% related
Iodide
17.7K papers, 300.9K citations
72% related
Silica gel
22.3K papers, 325.5K citations
71% related
High-performance liquid chromatography
47.3K papers, 1M citations
71% related
Bromide
24.5K papers, 349.7K citations
71% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20235
202224
202117
20208
201912
201812