Showing papers on "Buffer solution published in 1980"
Patent•
27 Aug 1980
TL;DR: A buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of Tris; an acid having the formula R i -CO-NH -R 2 -COOH where R 1 is NH 2 or an alkyl or aryl group, preferably NH 2, C 6 H 5 or C 6H 4 NH 2 and where R 2 is an alyl group and a water soluble salt of the acid; the acid and salt being present in amounts and in a ratio to maintain the pH of an aqueous solution
Abstract: A buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of Tris; an acid having the formula R i -CO-NH -R 2 -COOH where R 1 is NH 2 or an alkyl or aryl group, preferably NH 2 , C 6 H 5 or C 6 H 4 NH 2 and where R 2 is an alkyl group, preferably CH 2 , CH 2 -CH 2 or CH(CH 3 ); and a water soluble salt of the acid; the acid and salt being present in amounts and in a ratio to maintain the pH of an aqueous solution of the composition at from 8.2. to 9.0. The preferred acid is hippuric acid. This application is a continuation-in-part of application Serial No. 92,250 filed 11-7-79 entitled "Buffer Composition and Method for the Electrophoretic Separation of Proteins", the entire disclosure of which is incorporated herein by reference.
26 citations
TL;DR: Comparison of freely stirred and mounted enzymatic membrane enabled us to determine an equivalent working area at high glucose concentration, and the importance of diffusional limitations on the overall reaction rates were determined.
24 citations
TL;DR: Fraction 1 protein was extracted from alfalfa and the purified protein identified with the enzyme ribulose-1,5-bisphosphate carboxylase and the presence of a single endotherm suggests that the subunits are tightly bound to each other and are denatured as a single unit.
19 citations
Patent•
01 Aug 1980
TL;DR: In this paper, the authors used a mixture of polyhydroxy organic compound and TRITON-X-100 to obtain a stabilized enzyme solution which can be used in combination with a chromogen diluent solution, which is made by dissolving phenol and triton-100 in water or buffer solution.
Abstract: Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by dissolving a salt of cholic acid in a buffer solution providing a pH within the range of about 4 to about 9, to the solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X-100. A cholesterol oxidase and a peroxidase are each dissolved in separate portions of buffer solution and introduced into the buffered solution containing the cholesterol esterase. 4-aminoantipyrine is then added to the solution. The resultant solution is a stabilized enzyme solution which can be used in the total cholesterol assay of a serum sample. The stabilized solution can be used in combination with a chromogen diluent solution which is made by dissolving phenol and TRITON-X-100 in water or a buffer solution. The combination of the stabilized enzyme solution and the chromogen diluent solution provides a solution which has utility in the spectro-photometric assay of total cholesterol.
19 citations
TL;DR: In both the rat and the hamster, the absorption of capsaicin was proportional to its concentrations in the mucosal medium and it was concluded that it was the soluble, and not particulate, Capsaicin which was absorbed and that passive diffusion could account for the intestinal absorption.
16 citations
TL;DR: In this article, a procedure for the calculation of the ionic strength and buffer capacity of a series of buffers prepared by the partial neutralization of a mixture of mono- and polybasic acids is outlined and data tabulated for a mixture 0.02 M in each of CH3COOH, H3PO4, and H3BO3.
15 citations
TL;DR: The effect of temperature and partial pressure of oxygen on antimony (Sb) microelectrodes was examined, and pH of blood was estimated in the bullfrog, with the difference between the two being relatively minor.
Abstract: With regard to pH measurement of biological fluids in vivo with metal-metal oxide microelectrodes, the effect of temperature and partial pressure of oxygen on antimony (Sb) microelectrodes was examined, and pH of blood was estimated in the bullfrog. The temperature coefficient (dE/dt) of electromotive force (EMF) of Sb-microelectrodes in the range of 7 to 37 degrees C was -1.18 +/- 0.113 mV/degrees C (mean +/- SEM) in Ringer solution, whereas that of the pH glass electrode in the same solution was -0.43 +/- 0.035 mV/degrees C. When estimated in Tris buffer solution, it was -0.06 +/- 0.063 mV/degrees C for Sb-microelectrodes and 1.05 + 0.036 mV/degrees C for glass electrodes. The change of slope constant (alpha in -mV/pH) in the Sb-microelectrode due to temperature change could be predicted empirically from: alpha = 0.40 (t-25) + 55.3, where t represents the measuring temperature in degrees C. The resultant deviation of pH reading between Sb and glass electrodes, delta pHSb-Glass, may be expressed by: delta pHSb-Glass = 0.00183 (t-25) +0.016. In the range of 45 to 760 mmHg of oxygen partial pressure it fixed pH, the EMF increased linearly with the increase of Po2, the slope (dE/dlog(Po2)) being 11.7 +/- 0.42 (SEM) mV (n = 13, t = 25 degrees C). In consideration of the above effects, the blood pH of bullfrog was estimated to be 7.697 +/- 0.092 (SD) and 7.729 +/- 0.111 with glass and Sb-microelectrodes respectively, the difference between the two being relatively minor.
14 citations
TL;DR: In this paper, a method for the detection of carboxylic acids in the effluent from ion exchange columns, based on the deviation of the pH of a buffer solution on the addition of Bronsted bases, is described.
14 citations
TL;DR: In this paper, it is suggested that published K2 values for succinic acid in water that were derived from the Harned cell are ≈ 2 % too high, provided data are obtained down to an ionic strength ≈ 0.01, pK2 values show little dependence on reasonable estimates of the parameters used in the Debye-Huckel activity coefficient expression.
Abstract: pK1 and pK2 values at zero ionic strength of some dicarboxylic acids in water and in 10 % ethanol (by volume) at 25 and 40°C have been compiled from e.m.f. measurements of a glass electrode, HCl, buffer, AgCl cell. As in previous work (Part 12) dilute HCl was used to calibrate the cell and the pKs assessed after additions of stock buffer solution. Critical comparisons with published figures for malonic and succinic acids in water, obtained from measurements with the classical Harned cell H2, Pt, buffer, NaCl, AgCl show that the glass electrode cell can yield answers of similar precision. Provided data are obtained down to an ionic strength ≈ 0.01, pK2 values show little dependence (≈ 1 %) on reasonable estimates of the parameters used in the Debye–Huckel activity coefficient expression. It is suggested that published K2 values for succinic acid in water that were derived from the Harned cell are ≈ 2 % too high.
13 citations
Patent•
14 Feb 1980
TL;DR: In this paper, a narrow or spleen of a domestic animal is dipped into a buffer solution in the pH of 3.4-7.5 or in 30-99.5 wt.% alcohol solution to prepare a dipped solution, which is dialyzed for a night.
Abstract: PURPOSE:To separate a biologically active material for use as a stimulating material in the segeneration of heart muscle and a growth controlling material, by purifying a manow or spleen of domestic animal, followed by treatment. CONSTITUTION:A narrow or spleen of a domestic animal is dipped into a buffer solution in the pH of 3.4-7.5 or in 30-99.5 wt.% alcohol solution to prepare a dipped solution, which is dialyzed for a night. Hydrogen sulfide gas is introduced into the dialyzed solution so that iron is precipitated and removed. Colored impurities are removed by adding 0.01-0.5 wt.% based on a liquid amount of active carbon to the solution or by using a decoloring resin. The solution thus obtained is directly subjected to freeze drying or after being passed through Sephadex or an ion exchange resin to separate biologically active material. This material is yellow solid powder, has 14 wt.% of nitrogen, and consists mainly of a protein exhibiting a maximum absorption in a wave length of 270-280 mm.
10 citations
TL;DR: In this paper, it was shown that a positive shift of the electrode potential is not associated with the growth of the passive film, provided that the rest potential is more positive than the Flade potential.
Patent•
12 Jan 1980
TL;DR: A 16-membered macrolide derivative of formula I (A is II, III, etc.; R is H, acetyl, propionyl, n-butylyl, etc.) and its acid addition salt was used for infectious diseases of Gram-positive bacteria or Mycoplasma as mentioned in this paper.
Abstract: NEW MATERIAL:A 16-membered macrolide derivative of formula I (A is II, III, etc.; R is H, acetyl, propionyl, n-butylyl, etc.) and its acid addition salt. EXAMPLE:The compound of formula IV. USE:Remedy for infectious diseases of Gram-positive bacteria or Mycoplasma. The concentration of the compound in blood is higher than that of the starting material. PROCESS:The compound I is prepared by reacting a 16-membered macrolide antibiotic substance V with a cultured medium, cells, or their treated product of microorganisms belonging to actionomycetes (e.g. Streptomyces albireticuli, etc.) in an aqueous medium (a phosphate buffer solution, sodium carbonate buffer solution, etc.) at pH 4-10, in the absence of oxygen (in an atmosphere of inert gas such as nitrogen, argon, etc.) with or without agitation, at 15-45 deg.C, pref. 25-35 deg.C.
TL;DR: The rates of the hydrolysis of 2-valeryloxy (C5)- and 2-heptanoyloxy(C7)-benzoic acids catalyzed by α-chymotrypsin (α-CHT) and the volume changes accompanying the dissociation of the enzyme–substrate complex and the activation volume for the process of the product formation were calculated.
Abstract: The rates of the hydrolysis of 2-valeryloxy(C5)- and 2-heptanoyloxy(C7)-benzoic acids catalyzed by α-chymotrypsin (α-CHT) were measured at pressures up to 2000 bar at 30 °C and pH 78 in a 005 M Tris buffer solution The apparent Michaelis constant, Kmapp, was estimated to vary from 59 to 99 mM, and kcat from 11×10−3 to 52×10−3 s−1, for the C5 ester, and Kmapp, from 47 to 10 mM, and kcat from 37×10−3 to 200×10−3 s−1, for the C7 ester, between 1 and 2000 bar From the pressure dependences of Kmapp and kcat, the volume changes accompanying the dissociation of the enzyme–substrate complex and the activation volume for the process of the product formation were calculated to be −63±2 and −20±2 cm3/mol for the C5 ester and −95±2 and −21±2 cm3/mol for the C7 ester The effects of the pressure on the hydrolysis of p-nitrophenyl acetate (PNPA) catalyzed by α-CHT have also been measured up to 3000 bar at pH 78 in a 005 M Tris buffer solution and at 25 °C and 35 °C The activation volumes were −3 cm3/mol at
01 Jan 1980-Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes
TL;DR: The cleavage of the N-S bond in 2,3-dihydro-2,2-dimethyl-7-benzofuranyl (di-n-butylaminosulfenyl) (methyl)carbamate was examined in different buffer solutions (hydrolysis), in buffer solution containing sulfhydryl reagents (thiolysis) and on thin-layer chromatographic plates.
Abstract: The cleavage of the N-S bond in 2,3-dihydro-2,2-dimethyl-7-benzofuranyl (di-n-butylaminosulfenyl) (methyl)carbamate was examined in different buffer solutions (hydrolysis), in buffer solution containing sulfhydryl reagents (thiolysis) and on thin-layer chromatographic plates. In buffer solution and on thin-layer plates, N-S bond cleavage readily occurred to give carbofuran as a major product, with minor amounts of bis-carbofuran-N,N'-disulfide and -trisulfide. The hydrolysis reaction in buffer proceeded with first-order kinetics. Significant amounts of an unknown polar compound were obtained in buffer solution and on thin-layer plates. In the presence of excess cysteine and glutathione at pH 7.0, thiolytic N-S bond cleavage occurred with first-order kinetics to give carbofuran as the sole identifiable product. At pH 5.0, three minor products were obtained along with carbofuran.
TL;DR: In this paper, the stability of binuclear copper(II) complex with 3,6-Dioxaoctane-1,8-diamine-N,N, N′,N′-tetraacetric acetic acid (ethylene glycol bis[bis(carboxymethyl)amino] ethyl] ether, customarily abbreviated to EGTA of Y) was determined on the basis of potentiometric titration by means of a copper ion-selective electrode.
Abstract: The stability constant of binuclear copper(II) complex with 3,6-Dioxaoctane-1,8-diamine-N,N,N′,N′-tetraacetric acetic acid (ethylene glycol bis[2-[bis(carboxymethyl)amino] ethyl] ether, customarily abbreviated to EGTA of Y) was determined on the basis of potentiometric titration by means of a copper(II) ion-selective electrode. In acetate buffer solution of pH 5.8 titration curves of copper(II) with EGTA show two distinct end points corresponding to Cu2Y and CuY complexes. The formation constant of the binuclear copper(II) complex was determined from the potential response in the titration fraction between first and second end points. The logarithmic value was found to be log KCu2Y=6.40 at ionic strength of 0.2 M (KNO3–acetate buffer solution) at 25±0.5 °C.
Patent•
18 Nov 1980
TL;DR: In this article, a photo-sensor was used to obtain a water-soluble photo-sensitive material capable of providing a resist film superior in photosensitivity and resolution, good in dyability and corrosion resistance, by using the hydrolysis product of a mammalian collagen having a specified molecular weight and a specified viscosity range.
Abstract: PURPOSE: To obtain a water-soluble photosensitive material capable of providing a resist film superior in photosensitivity and resolution, good in dyability and corrosion resistance, by using the hydrolysis product of a mammalian collagen having a specified molecular weight and a specified viscosity range, and a photocrosslinkable photosensitive agent. CONSTITUTION: A hydrolysis product of collagen made from the bones and hides of an ox, pig, or the like retains coiling tendency of its molecular chain even in hydrolyzing its main peptide chain to give 2,000W30,000 number average molecular weight Mn, and 0.060W0.155dl/g intrinsic viscosity (η) at 40°C in a 0.15mol/ l citric acid buffer solution. This product is mixed with a photosensitive agent for causing a photocrosslinking reaction with a peptide resin on active light irradiation, such as bichromate or diazonium salt to give a water-soluble mixture, which is coated on a glass base, dried, brought into contact with an original plate, exposed to light, developed with water, and dyed to form a resist image having high resolution and high color density, and a superior corrosion resistance. COPYRIGHT: (C)1982,JPO&Japio
Patent•
20 Nov 1980TL;DR: In this paper, the α-2-SB-glycoprotein was obtained from physiological solutions thereof by binding to a bindable protein immobilized on an insoluble carrier, wherein the carrier-fixed protein was subsequently washed with a buffer solution of pH 6 to 8.5 and thereafter, the α 2-SB glycoprotein is eluted with buffer solution with a pH of 9 or more and isolated from the eluate.
Abstract: The present invention provides a process for obtaining α-2-SB-glycoprotein from physiological solutions thereof by binding to a bindable protein immobilized on an insoluble carrier, wherein the carrier-fixed protein is subsequently washed with a buffer solution of pH 6 to 8.5 and thereafter the α-2-SB-glycoprotein is eluted with a buffer solution with a pH of 9 or more and isolated from the eluate.
Patent•
28 Aug 1980
TL;DR: In this paper, a caviar-like food product was created using alignic acid, LM-pectin or their mixture and the equal amount of isolated soybean protein in the coagulation solution.
Abstract: PURPOSE:An aqueous solution containing alignic acid, LM-pectin or their mixture and the equal amount of isolated soybean protein is dropped in the coagulation solution to form particles and heated to produce a caviar-like food product. CONSTITUTION:To 100pts.wt. of a water-soluble salt of alginic acid, LM-pectin or their mixture, are added 60-130pts. of isolated soybean protein and further, casein, gelatin, HM-pectin, pigment and flavors are combined. Then, they are dispersed and dissolved in water. The mixture is dropped in a solution of a salt of divalent or morevalent metal other than Mg and Hg to form particles. The particles are dipped in a buffer solution of 2.0-4.0pH to effect coloration and the seasoning. At this time, a tannin-containing salt is used as a colorant and particles are dipped in the aqueous solution and further in a solution of divalent metal salt other than Mg, Hg, whereby the pigment is fixed.
Patent•
[...]
15 Mar 1980
TL;DR: In this paper, the authors proposed to suppress the proliferation of bacteria in foods, without lowering the taste, by adding an acetate buffer solution (pH4.2-5.0) composed of 1 mole of an acetic acid source such as brewed vinegar, synthetic vinegar, etc.
Abstract: PURPOSE:To suppress the proliferation of bacteria in foods, without lowering the taste, by adding an acetate buffer solution. CONSTITUTION:Food (e.g. pickles, jelly, noodles, boiled food, roast food, cakes, etc.) is preserved by adding 0.01-0.5mol% of an acetate buffer solution (pH4.2- 5.0) composed of (A) 1 mole of an acetic acid source such as brewed vinegar, synthetic vinegar, etc., and (B) 1/3-3moles of a salt or base comprising an acetate, carbonate, bicarbonate, or hydroxide of Na, K, Ca, or Mg, or salt water.
TL;DR: In this article, a method for estimation of cyanide is suggested, based on first-order reactions with the same K value of 0.066 hr−1, but with opposite sign.
Abstract: The decomposition of cyanide-pyridine-barbituric acid in the wavelength around λmax=583 nm gives rise to formation of a new color species around λmax=490nm. Both reactions are first-order reactions with the same K value of 0.066 hr−1, but with opposite sign. The pH value, the nature and concentration of the buffer solution influence absorption to a considerable extent. A method for estimation of cyanide is suggested.
TL;DR: In this article, the potential of a sulfide ion-selective electrode (SISE) for the determination of lead(II) at different pH values is presented, and a relationship between the initial rate of decrease of the sulfide concentration in test solution and various amounts of lead is discussed.
Abstract: The possibilities of a sulfide ion-selective electrode (SISE) for the determination of lead(II) at different pH values is presented. Potential - time curves recorded after addition of lead(II) in sulfide solution constitute the primary data in this study. The influence of the pH and ethanol in ethanol-aqueous mixtures on the behaviour of a SISE and on formation of PbS was investigated. A relationship between the initial rate of decrease of the sulfide concentration in test solution and various amounts of lead(II) is discussed. The results of measurements showed the possibility of determining up to micromole quantities of lead(II).
Patent•
15 Dec 1980
TL;DR: In this paper, the authors measured the total polyamines rapidly with ease by oxidatively decomposing ascorbic acid in a sample, oxidizing a free and conjugation type polyamines with a specific enzyme, reacting the resultant H2O2 with a chromogen, and optically measuring the resultant substance.
Abstract: PURPOSE:To measure the total polyamines rapidly with ease, by oxidatively decomposing ascorbic acid in a sample, oxidizing a free and a conjugation type polyamines with a specific enzyme, reacting the resultant H2O2 with a chromogen, and optically measuring the resultant substance CONSTITUTION:001-020ml sample is added to a solution prepared by dissolving 01-50U/ml ascorbic acid oxidase in 05-20ml buffer solution of 55- 75pH and 002-010 M concentration, and reacted at 20-40 degC for 3min or longer to decompose oxidatively the ascorbic acid in the sample A solution containing 20-50U/ml enzyme, eg polyamine oxidase, capable of oxidizing free and conjugation type polyamines to form H2O2 in 10-40ml buffer solution of 70-90pH and 005-02 M concentration, and 01-50U/ml enzyme having the peroxidase-like activity and a chromogen, eg homovanilic acid, is added thereto to oxidize the free and conjugation type polyamines and react the formed H2O2 with the chromogen The resultant substance is then colorimetrically measured
Patent•
15 Dec 1980
TL;DR: In this article, cyclodextrin is added to a buffer solution containing a maltooligosaccharide having phenyl halide groups linked to the reducing terminal groups as a substrate.
Abstract: PURPOSE: To measure the titled substance accurately, by adding cyclodextrin to a buffer solution containing a maltooligosaccharide having phenyl halide groups linked to the reducing terminal groups as a substrate. CONSTITUTION: In dissolving a maltooligosaccharide having phenyl halide groups linked to the reducing terminal groups in a buffer solution, cyclodextrin is added thereto. The amount of the cyclodextrin is about 0.5W5wt% based on the buffer solution. A sample is then reacted with the resultant substrate solution, and then α-glucosidase and β-glucosidase are reacted therewith to measure the liberated halogenophenol. Thus, the amylase activity in the sample can be measured accurately. COPYRIGHT: (C)1982,JPO&Japio
Patent•
12 Apr 1980
TL;DR: In this paper, the authors proposed to decrease the unevenness in treatment due to the contact of bubbles in the process of surface treatment by moving a thin plate between the first and the second holders which constitute a combined body with the body being rotated, and by exposing one half of the thin plate from the surface level of the treatment solution.
Abstract: PURPOSE:To decrease the unevenness in treatment due to the contact of bubbles in the process of surface treatment by moving a thin plate between the first and the second holders which constitute a combined body with the body being rotated, and by exposing one half of the thin plate from the surface level of the treatment solution. CONSTITUTION:A semiconductor wafer 2 is inserted into a holder 1A and a holder 1B is attached to the other side in an inverted fashion, thereby a combined body 11 is constituted. Both holders are fixed by engage locks 12a and 12b, and shafts 13a and 13b are attached. The holder 1A is immersed in the buffer solution 9 in a tank 8 so that the contact plane of the holder 1A and 1B appears slightly above the surface of the solution 9. When the combined body rotates on the shafts 13a and 13b, at least one half of the wafer is exposed above the solution and moves from the holder 1A to the holder 1B. The bubbles, which are yielded and attached to the wafer in the etching process during which the wafer is immersed in the buffer solution, are spontaneously eliminated by the rotation, and unevenness caused by bubbles during the etching process is not yielded.
Patent•
06 Mar 1980
TL;DR: In this paper, the passive latex cohesion reaction was used to measure the antigen streptolysin O-valve sharply and specifically by coupling the latex particles with staphylsine O.
Abstract: PURPOSE:To manufacture the latex for soluble linked fungus infection diagnosis which can measure the antigen streptolysin O-valve sharply and specifically by using the passive latex cohesion reaction, by coupling the latex particles with streptolysine O. CONSTITUTION:The deposit obtained by adding soluble linked fungus cultured filter solution to the solid ammonium sulfate is dissolved to water, and after removing the ammonium sulfate by dialysis of this solution to water, the sephadex G-100 balanced with the phosphate buffer salt water (PBS) is used, gel filter is made, streptolysin O(SLO) is collected to obtain refined SLO. Next, the latex particles (favorably, diameter about 1mu, specific gravity about 1.15 to 1.25 polystylene latex particles) is suspended in the buffer solution of neutral such as PBS, the buffer solution of refined SLO is added to it, it is processed at room temperature for 1 to several hours, to obtain the titled sensing latex.
Patent•
25 Jul 1980
TL;DR: In this article, a gamma-globulin derivative composed mainly of the monomer, having low anticomplimentary action, and administrable by intravenous injection, easily, in an industrial scale, by treating a sulfonated gamma-lobulin in a buffer solution for development with an ion exchanger, thereby removing coagulated globulin molecules.
Abstract: PURPOSE:To prepare a gamma-globulin derivative composed mainly of the monomer, having low anticomplimentary action, and administrable by intravenous injection, easily, in an industrial scale, by treating a sulfonated gamma-globulin in a buffer solution for development with an ion exchanger, thereby removing coagulated globulin molecules. CONSTITUTION:A solfonated gamma-globulin is treated either with an anion exchanger (e.g. DEAE-Sepharose) in a buffer solution for developing use having a pH of 4-10 and an ionic strength of 0.01-0.15, or with a cationic exchanger in a buffer solution having a pH of 4-6.5 and an ionic strength of 0.01-0.1. Most of the monomolecular sulfonated gamma-globulin molecules are adsorbed to the ion exchanger by the treatment, and the coagulated sulfonated gamma-globulin and the non-sulfonated gamma-globulin molecules are passed through the ion exchanger unabsorbed. The absorbed monomolecular sulfonated gamma-globulin molecules are eluted with a buffer solution having a pH of 3-8 (anionic exchanger) or of 6-9 (cationic exchanger). The recovered sulfonated gamma-globulin is purified by conventional process.
TL;DR: In this article, cross-linked poly (4-vinylpyridinium) and α, ω -dibromides were used as cationic catalysts for the decarboxylation of 6-nitrobenzisoxazole-3-carboxylate anion.
Abstract: Catalysis of cationic polyelectrolytes for the decarboxylation of 6-nitrobenzisoxazole-3-carboxylate anion was studied in a buffer solution (pH=9.0). Cross-linked poly (4-vinylpyridinium) salts prepared from 4-vinylpyridine and α, ω -dibromides were used as cationic catalysts. The cross-linked catalysts were found to accelerate markedly the decarboxylation in comparison with the linear water-soluble analogues. Effect of the polymer structure such as the length of (CH2)x linkages between positive charges on the catalytic activity was examined. It was suggested that the acceleration by the cross-linked polymer catalysts would be due to the hydrophobic microenvironment around the catalytic sites.
Patent•
28 May 1980
TL;DR: In this article, the buffer solution is fed to the dialysis chamber 23 of the unit 20 and the inlet of specimen 4 with the delivery pumps 3a and 3b.
Abstract: PURPOSE:To extend the range possible for analysis, by leading the buffer solution in two flows into a pair of dialysis chamber of the dialysis unit sectioned with dialysis film, and making greater the flow of the buffer solution of the side passing through the fixed enzyme column. CONSTITUTION:The buffer solution 2 is fed to the dialysis chamber 23 of the dialysis unit 20 and the inlet of specimen 4 with the delivery pumps 3a and 3b. The buffer solution 2 through the inlet of specimen 4 is discharged through the dialysis chamber 22. The buffer solution 2 including the substrate and through the dialysis chamber B23 passes through the fixed enzyme column 7 and after it is detected at the engyme detector 10, it is discharged as the discharge liquid 11b. The reduction in the engyme detected analyzes the substrate in the specimen. The dialysis chamber 20 is sectioned with the dialysis film 21 and the flow of the buffer solution of the dialysis chamber B23 is taken greater than that of the chamber A22. Thus, the flow of the buffer solution in the column is increased to extend the range of possible analysis.
Patent•
14 Jul 1980
TL;DR: In this article, a reagent for the detection of peroxidase, having high performance comparable to benzidine, was provided by dissolving a specific hydrazone and a phenolic compound in a buffer solution.
Abstract: PURPOSE:To provide a reagent for the detection of peroxidase, having high performance comparable to benzidine, and prepared by dissolving a specific hydrazone and a phenolic compound in a buffer solution. CONSTITUTION:The title reagent is obtained by dissolving (A) 0.5-5m-mol of 3-methyl-2-benzothiazolinone hydrazone hydrochloride and (B) 3-25m-mol of a phenolic compound of formula (A is NH2, NHCH3, N(CH3)2, OCH3, or halogen; B is CH3, H, or halogen; X is HCl, H2SO4, CH3COOH, or HCOOH; n is 0, 1, or 2), e.g. o-aminophenol, 2,3-dichlorophenol, etc. in (C) a buffer solution, e.g. a phosphate buffer solution, and adjusting the pH of the mixture to 5-7.
Patent•
07 Jan 1980
TL;DR: In this article, an inner layer of a europium activator acid sulfide fluorescent (EAAS) and an outer layer of an EAAS was used to improve the stability of a colored fluorescent substance by washing it with a buffer solution having a specific pH.
Abstract: PURPOSE:To improve stability of a colored fluorescent substance, consisting of an inner layer of a europium activator acid sulfide fluorescent substance and an outer layer of a europium activation sulfide fluorescent substance, in a water slurry, by washing it with a buffer solution having a specific pH. CONSTITUTION:A europium activator acid sulfide fluorescent substance shown by the formula II (Ln is Y, Gd, La, Lu, or Sc) is blended with a calcium salt, e.g., CaO or CaS, and a strontium salt, e.g., SrO, or SrS, and sintered at about 1000-1500 deg.C for ten minutes-5 hours to form a red colored fluorescent substance consisting of a fluorescent substance shown by the formula II as an inner part and a europium activation sulfide fluorescent substance shown by the formula I (0<=x<=0.75) on the surface layer. The red colored fluorescent substance is washed with a buffer solution (e.g., acetic acid-ammonium acetate) in the pH of 6-8 (preferably 6.5-7.5) at 0-70 deg.C for several minutes-two hours.