Topic
Buffer solution
About: Buffer solution is a research topic. Over the lifetime, 6948 publications have been published within this topic receiving 112440 citations. The topic is also known as: pH buffer & buffer.
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TL;DR: In this article, Nafion membranes were deposited on the top of the glucose ENFETs by a spin-coating procedure, which resulted in a substantial reduction of the effect of the buffer concentration on the EN-FET's response and in an extension of the dynamic range of the sensor up to concentrations of more than 10 mM glucose.
51 citations
TL;DR: In this paper, a novel Nafion-cysteine functional membrane was constructed and electron transfer of horseradish peroxidase, Euphorbia latex amine oxidase, superoxide dismutase and cytochrome c was carried out on the functional membrane modified gold electrode.
51 citations
TL;DR: Rapidity, precision, and good selectivity were found for the determination of pantoprazole in pharmaceutical dosage forms and human plasma and high-performance liquid chromatography with a diode array and UV/VIS detection at 290.0 nm determination also was developed.
51 citations
TL;DR: The results proved the efficacy of the fabricated modified electrode for simple, low cost and highly sensitive medicine sensor well suited for the accurate determinations of trace amounts of rifampicin in the pharmaceutical and clinical preparations.
51 citations
TL;DR: The results suggest that the nitrate anion is not formed by a direct isomerization of OONO(-), but by an indirect route originating from NO(-).
Abstract: The mechanism of decomposition of peroxynitrite (OONO−) in aqueous sodium phosphate buffer solution at neutral pH was investigated. The OONO− was synthesized by directly reacting nitric oxide with superoxide anion at pH 13. The hypothesis was explored that OONO−, after protonation at pH 7.0 to HOONO, decomposes into 1O2 and HNO according to a spin-conserved unimolecular mechanism. Small aliquots of the concentrated alkaline OONO− solution were added to a buffer solution (final pH 7.0–7.2), and the formation of 1O2 and NO− in high yields was observed. The 1O2 generated was trapped as the transannular peroxide (DPAO2) of 9,10-diphenylanthracene (DPA) dissolved in carbon tetrachloride. The nitroxyl anion (NO−) formed from HNO (pKa 4.5) was trapped as nitrosylhemoglobin (HbNO) in an aqueous methemoglobin (MetHb) solution. In the presence of 25 mM sodium bicarbonate, which is known to accelerate the rate of decomposition of OONO−, the amount of singlet oxygen trapped was reduced by a factor of ≈2 whereas the yield of trapping of NO− by methemoglobin remained unaffected. Because NO3− is known to be the ultimate decomposition product of OONO−, these results suggest that the nitrate anion is not formed by a direct isomerization of OONO−, but by an indirect route originating from NO−.
51 citations